Volume 68 Issue 1

  1. Sang Yeon Lee 9
    https://doi.org/10.5012/jkcs.2024.68.1.9 PDF HTML
  2. Yubin An;Sunwoo Lee;Jonghoon Oh 12
    https://doi.org/10.5012/jkcs.2024.68.1.12 PDF HTML
  3. Munseok S. Chae 15
    https://doi.org/10.5012/jkcs.2024.68.1.15 PDF HTML
  4. U. K. Abdurakhmanova;M. R. Askarova;H. K. Egamberdiev 20
    https://doi.org/10.5012/jkcs.2024.68.1.20 PDF HTML
    This article presents the advantages of utilizing gossypol and its derivatives as reagents for iron (III) (Fe (III)) ions. A novel spectrophotometric method has been developed for the determination of Fe (III) using gossypol derivatives in the presence of a universal buffer solution. Optimal conditions have been identified, and the composition and stability constants of the Fe (III) complex with gossypolacetic acid have been determined.
  5. Thang Quyet Nguyen;Wonchull Kang 25
    https://doi.org/10.5012/jkcs.2024.68.1.25 PDF HTML
    The p97 adenosine triphosphatase is a key player in protein homeostasis, responsible for unfolding ubiquitylated substrates. It engages with various adaptor proteins through its N-terminal domain, with the p97-p47 complex attracting particular attention for its involvement in membrane remodeling. Although the structures of p97 in complex with the Ubiquitin regulatory X (UBX) domain from various adaptors have been reported, the stoichiometry is conflicting. Here, we report the crystal structure of the p97 N-D1 hexamer in complex with the p47 UBX domain at a resolution of 2.7 Å. The structure reveals a stoichiometry of 6:6 between the p97 N-D1 and the p47 UBX domain. These findings provide valuable insights into the binding stoichiometry of p97 N-D1 and p47 UBX domain, which are crucial for understanding the role of p97 and adaptor proteins in cellular processes such as the ubiquitin-proteasome pathway, membrane fusion, and cell cycle regulation.
  6. Gyeong Soo Ko;Thang Quyet Nguyen;Seri Koh;Wonchull Kang 32
    https://doi.org/10.5012/jkcs.2024.68.1.32 PDF HTML
    Glycerol dehydrogenase (GlyDH) plays a crucial role in the glycerol metabolism pathway by catalyzing the oxidation of glycerol to dihydroxyacetone (DHA). Previous studies of GlyDH have predominantly focused on unraveling the structural features of the active site and its binding interactions with ligand. However, the structural details of GlyDH in complex with both NAD+ and the substrate bound have remained elusive. In this study, we present the crystal structures of Klebsiella pneumoniae GlyDH (KpGlyDH) in the absence and presence of NAD+ at a resolution of 2.1 Å. Notably, both structures reveal the binding of the substrate, ethylene glycol, to the zinc ion. Interestingly, a significant change in the coordination number of the zinc ion is observed, with three in the absence of NAD+ and four in its presence. These findings shed light on the structural aspects of GlyDH and its interactions with NAD+ and the substrate.
  7. Jihun Park;Sunhyang Park;Jeonghee Nam 40
    https://doi.org/10.5012/jkcs.2024.68.1.40 PDF HTML
    이 연구는 화학 핵심 개념, 화학 행동 영역, 수학 행동 영역으로 구성된 3차원 분석틀을 이용하여 2019학년도부터 2022학년도까지 4개년도의 대학수학능력시험 화학 I 문항을 분석하여 영역별 출제 문항 수와 비율을 알아보고 이를 바탕으로 각 영역 간의 관계에 대하여 알아보았다. 연구 결과, 대학수학능력시험 화학 I 문항은 핵심 개별 별로 비교적 고르게 출제되었으나, 고난도 문항은 양적 관계에 편중되었다. 화학 행동 차원의 경우, 문제 인식 및 가설 설정, 탐구 설계 및 수행 영역의 문항 출제 비율이 매우 낮았고, 고난도 문항의 경우 결론 도출 및 평가에 편중되었다. 수학 행동 영역의 경우, 발견적 추론 영역과 연역적 추론 영역에서 출제된 문항 수가 매우 적었고, 고난도 문항은 내적 문제해결력 영역에 편중되었다. 또한, 특정 화학 핵심 개념과 화학의 행동 영역은 특정 수학 행동 영역과 높은 연관성을 보였으며, 이러한 특징은 학생들이 어려워하는 고차원적 수학 행동 영역을 포함하는 문항에서 두드러지게 나타났다.
  8. Hahkjoon Kim 54
    https://doi.org/10.5012/jkcs.2024.68.1.54 PDF HTML