This study analyzed facts affecting the performance of refrigeration systems after throttling actions, by changing the amount of refrigerant according to adjusting the opening of manual expansion valve to 80%, or 20% with vapor compressional refrigeration training equipment. At opening of 20%, the inlet and outlet temperature of compressor, subcooling and superheat, condenser heat, refrigeration effect, flash gas heat, coefficient of performance were higher, while at 80%, condensing pressure, evaporating pressure, compression work were higher, Thus, we could see changes in the amount of refrigerant affect the performance of the refrigeration system.