In this study, based on the effect of the interaction of fracture mechanics by graphite and fatigue limit phenomena of the microscopic observation various matrix structure, spheroidal ratio, size of graphite and distribution etc. parameters containd with Ductile Cast Iron. Therefore, in this study, different ferrite-pearlite matrix structure and spheroidal ratio of graphite of 70%, 80% and 90%, GCD40, GCD45-1 and GCD45-2 series and three different ferrite-pearlite matrix structure, GCD 45-3, GCD 50, GCD 60 series, all of which contain more than 90% spheroidal ratio of graphite, were used to obtain the correlation between mean size of spheroidal graphite and fatigue strength. (1) 73% pearlite structure had the highest fatigue limitation while 36% pearlite structure had the lowest fatigue limitation among ferrite-pearlite matrix. the increase in spheroidal ratio with increasing fatigue limitation, 90% had the highest, 14.3% increasing more then 10%, distribution range of fatigue life was small in same stress level. (2) (equation omitted) of graphite can be used to predict fatigue limit of Ductile Cast Iron. The Statistical distribution of extreme values of (equation omitted) may be used as a guideline for the control of inclusion size in the steelmaking processes.