Xiexiafa is the one of eight methodes of treatment, the purgatives of oriental medicine was originated from Shanghanlun written by Zhangzhongjing. Traditionally Xiexiafa has been used to treat constipation. But All kinds of researches on Xiexiafa made purgatives possible to use variously for the disease of internal medicine. In the research on clinical use of Xiexiafa, the results were as follow. Xiexiafa is available on emergency medicine because of instant relief. It has an ability of preventive medicine and economical effect. It was considered to treat hard to cure and chronic diseases made by Sushi, Rexie, Shuijie, Tanzhi, Xuxie etc. Considering strong side effects of Xiexiafa and patient conditions, it must be used carefully.

Objective : The aim of this study was to investigate the neuroprotection effect of estrogen on brain atrophy following cerebral infarction. Method : All animals in this study were classified into 4 groups; ovariectomy group (OVXgroup), cerebral infarction group (INF group), combination ovariectomy and cerebral infarction group (OVX + INF group), and naturally intact group for control data (NOR group). Cerebral infarction was made by Chen's method with some modification. Ovariectomy was performed by Wayforth's method. Experimental data for each group was collected at 15 days, month, 3 months, and 6 months after starting observation. Serum $17{\beta}-estradiol(E2)$ was determined by radioimmunoassay. Brain volume was measured and calculated with image analysis. Each brain was sliced at intervals of 2mm in chamber after 30 min of freezing in refregerater. Cerebral volume was obtained by sum of volume of each slice level, which was mean $area{\;}{\times}{\;}2mm$. Results : Cerebral ischemia was found to decrease the serum concentration of $17{\beta}-{\;}estradiol(E2)$ and to inhibit the physiologically conpensatary function of the ovariectomized rats. Also we found that deprivation of estrogen have resulted in more severe cerebral atrophy followed by cerebral infarction. Conclusion : It is suggested that estrogen has a neuroprotection effect on cerebral atrophy following cerebral infarction.

Objective : Acupuncture and Radix Astragali aqua-acupuncture stimuli have long been used to cure human diseases. However, the exact physiological and biochemical mechanisms involved remain undiscovered. Thus, many attempts have been made to show the scientific mechanisms involved. The effects of acupuncture and Radix Astragali aqua-acupuncture, which was known to date, as follow; effective circulation of body blood system and proliferation of leucocytes. Methods : In this study, we have applied acupuncture and Radix Astragali aqua-acupuncture stimuli to mouse on Sinsuhyul, a stimulative point of oriental medicine, to see effects on the expression of cytokine $IL-1{\beta}$. Mice were treated with lipopolysaccharide(LPS) for inflammation induction and then reverse transcriptase-polymerase chain reaction (RT-PCR) using each primer set were performed to trace the amounts of mRNA. Results : 1. $IL-1{\beta}$ was not expressed in LPS-nontreated mice at 15 to 60 min after acupuncture-stimuli. However, expression occurred after 3hrs. 2. $IL-1{\beta}$ was specifically expressed in LPS-treated mice at 30 min after acupuncture-stimuli. 3. $IL-1{\beta}$ was expressed in LPS-nontreated mice at 30 min after Radix Astragali aqua-acupuncture stimuli, however, not expressed at 60, 180 min. 4. $IL-1{\beta}$ was gradually expressed in LPS-treated mice at 15 to 180 min after Radix Astragali aqua-acupuncture stimuli. Conclusions : $IL-1{\beta}$ in LPS-treated mice was more effective than that of LPS-nontreated mice. We are now in the process of elucidating the immunological action mechanism of acupuncture and Radix Astragali aqua-acupuncture stimuli. And cytokine $IL-1{\beta}$ can be used not only as a basis of the effects of acupuncture and Radix Astragali aqua-acupuncture but also as a diagnosis guide through the immunological actions of those.

Objectives : In order to study experimentally the effects of Woogwi-eum(Yougui-yin) on hypothyroidism, ${T_3}-uptake$ content, $T_3$ content, $T_4$ content, TSH content, hematological values, enzyme activity, total cholesterol content, $Na^{+}$ content change in the serum and body weight of rats induced by thiouracil were measured after medication of extract of Woogwi-eum (Yougui-yin). Methods : Sparque-Dawley rats were used in this experiment and the rats were divided into normal, control and experimental groups. The hypothyroidism of control and experimental groups was induced by thiouracil, Woogwi-eum(Yougui-yin) extract was orally administered in the experimental groups for 20 days Results : In this experiment, the Woogwi-eum(Yougui-yin) treated group showed a significant increase in the serum ${T_3}-uptake$, TSH, RBC and hematocrit content, whereas a significant decrease in the serum CPK, ALP, LDH, total cholesterol content and the body weight was measured. Conclusions : According to the above results, it is suggested that Woogwi-eum(Yougui-yin) is partially effective to the hypothyroidism induced by thiouracil in rats. Therefore, Woogwi- eum(Yougui-yin) seems to be applicable to hypothyroidism in clinical practice.

Objective : The purpose of this study was to investigate the effects of Angelicae Gigantis Radix on brain damage and the cardiovascular system. Method : By administration Angelicae Gigantis Radix, we compared treated groups with not treated groups, in view of five points as follows $\circled1$ the intervascular eNOS, $\circled2$ the blood pressure of SHR, $\circled3$ the transcription of the Kir6.2, $\circled4$ the survival time of the rats when common carotid artery was ligated, and $\circled5$ antiplatelet aggregation. Result : In this experiment, Angelicae Gigantis Radix treated group showed significant produced in the intervascular eNOS and decreased the blood pressure of SHR, increased the transcription of the Kir6.2 and SUR significantly in all Treated groups, prolonged the survival time of the rats when common carotid artery, showed the effects on antiplatelet aggregation. Conclusion : According to the above results, Angelicae Gigantis Radix can protect the brain damage and have cardiovascular system effect.

Objectives: The purpose of this study was to investigate the antithrombotic effect of Carthamus tinctorius. Methods: SD rats were used to investigate the inhibitive effect of platelet aggregation (in vitro & in vivo), prothrombin time, platelet count. Mice were used to investigate the survival rate after injection of collagen & epinepbrine. Results: 1. The inhibitive effect of platelet aggregation (in vitro & in vivo) were increased significantly in all groups. 2. Prothrombin time was decreased in both groups. 3. Platelet count was decreased significantly in sample A. 4. Survival rate after injection of collagen & epinepbrine was increased in sample B. Conclusion: According to the above results, it is suggested that Carthamus tinctorius has antithrombotic effects.

Objectives : Fatigue is a common symptom experienced by everyone. Nevertheless, clinicians have a tendency of ignoring it since fatigue itself is not considered a distinct disease. Actually, some limited research about chronic fatigue syndrome has been made within the country, but in reality, the probability of getting this syndrome is still considered very low due to the strict diagnosis standard. Therefore, there are tremendous numbers of patients who do not get enough attention from clinicians for their fatigue symptoms only because technically they do not belong to the syndrome. Therefore, a basic statistical database must be compiled and patient management programs must be developed. To accomplish this, we conducted this study by measuring degree of fatigue, clinical characteristics and processes of Oriental medical treatment of fatigue patients. Methods : The objects of this study were selected from the new patients who entered the tonification Clinic in Kynnghee Oriental Medical Center between August 11, 2000 and October 7, 2000. Their main complaint was fatigue and they did not suffer from any physical or mental problem either historically or at the time of the study. The objects were divided into two groups based on duration of fatigue; fatigue under 6 months is considered as acute fatigue and fatigue for longer than 6 months is chronic fatigue. The prepared survey sheet for measuring fatigue degree was distributed to the patients with their consent. The patients were divided again into three subgroups : the fIrst group went through 1st test and constitution test after tonification clinic; the second one went into constitution test skipping Ist measuring test; the third one went into only tonification clinic with neither 1st measuring test nor constitution test. Results : The total number of object patients was 47 and 80% of them were considered as 'fatigue patients' by the Chalder scale. Among all patients, 29.5% requested treatment for chronic fatigue, which is over 6 months. The average of scale II for all patients was 14.8, which indicates moderate fatigue. The averages of scale II-1, II-2, II-3 were respectively 7.5, 5.9, and 3.7 so the most common complaint was physical fatigue. When compared scale II based on occupations, student group scored 6.9 and office man group scored 8.5 in scale II-1, physical fatigue, but it was not significant. Conclusions : Numerous number of patients have come to Oriental medical centers or hospitals in Korea. Therefore, deeper statistical research and follow-up-monitoring are reqnired in the Oriental medical academic world. In this study, among all patients who entered the tonification Clinic in Kyunghee Oriental Medical Center, 29.5% requested treatment for chronic fatigue, which is over 6 months. This kind of statistical report is the first time trial in the Oriental medical academy world. Through these steps, more objective treatment can be made and standards of prognosis assessment can be established.tablished.

Objectives : This study was designed to investigate the neuroprotective effect of Hwansodan(Huanshao-dan) on the apoptosis induced by withdrawal of neurotrophic support. Methods : PCl2 pheochromocytoma cells have been used extensively as a model for studying the cellular and molecular effects of neuronal cells. The viability of cells was measured by MTT assay. We used DNA fragmentation and caspase 3-like protease activation assay. Results : The water extract of Hwansodan(Huanshao-dan) significantly showed protective effects on serum and glucose deprivation-induced apoptotic death. Hwansodan(Huanshao-dan) also prevents DNA fragmentation and caspase 3-like protease activation, representing typical neuronal apoptotic phenomena in PCl2 pheochromocytoma cells and induces tyrosine phosphorylation of proteins around 44 kDa, which was identified as ERK1 with electrophoretic gel mobility shift by Western blot. In addition, MAPK kinase(MEK) inhibitor PD98059 and Ras inactivator, ${\alpha}-hydroxyfarnesylphosphonic$ acid attenuated the neuroprotective effects of Hwansodan(Huanshao-dan) in serum-deprived PCl2 cells. Conclusions : These results indicate that Ras/MEK/ERK signaling pathway plays a key role in neuroprotective effects of Hwansodan(Huanshao-dan) in serum-deprived PCl2 cells. Taken together, we suggest the possibility that Hwansodan(Huanshao-dan) might provide a neurotrophic-like activity in PCl2 cells.

Objectives: One of the most important and difficult things in SaSang Constitution Medicine is to diagnose and classify constitution. So there have been a lot of methods and tools to diagnose and classify it. We have attempted to show the reliability and clinical application of the QSCC Ⅱ (Questionnaire for the Sasang Constitution Classification Ⅱ), comparing with our own clinical research. Methods: We surveyed information from QSCC Ⅱ and compared QSCC Ⅱ' s reliability with clinical research of 54 patients' constitution(men 24, women 30). Results: Taeeum-in is the most commonly reported constitution by clinical research, and the next is Soeum-in. Soyang-in is the most commonly reported constitution by QSCC Ⅱ and the next is Taeeum-in. In Taeeum-in and Soeum-in' s items of QSCC Ⅱ, these matched those of the clinical research. Soyang-in' s items of QSCC Ⅱ did not match. Conclusions: The efficiency of QSCC Ⅱ is higher than clinical research in Taeeum-in, Soeum-in. But lower than clinical research, Soyang-in and Taeyang-in have no reliability in QSCC Ⅱ. So Soyang-in and Taeyang-in require alternative complementary studies in the future.

Objectives : The water extract of Sebsaeng-eum(SheShengYin) has been used for treatment of ischemic brain damage in oriental medicine, However, little is known about the mechanism by which the water extract of Sebsaeng-eum(SheShengYin) rescues brain cells from ischemic damages. Methods : To elucidate the protective mechanism on ischemic induced cytotoxicity, We investigated the regulation of LPS and PMA induced iNOS expression in $C_{6}$ glial cells. Results : LPS and PMA treatment for 48 h in $C_{6}$ glial cells markedly induced NO, but treatment of the cells with the water extract of Sebsaeng-eum(SheShengYin) decreased nitrite formation. In addition, LPS and PMA treatment for 48 h induced severe cell death in $C_{6}$ glial cells. However treatment of the cells with the water extract of Sebsaeng-eum(SheSheng Yin) did not induce significant changes compared to the control. LPS and PMA induced iNOS activation in $C_{6}$ glial cells caused chromosomal condensation and fragmentation of nuclei. Conclusions : Taken together, We suggest that the protective effects of the water extract of Sebsaeng-eum(SheShengYin) against ischemic brain damages may be mediated by regulation of iNOS during ischemic condition.

Objective: Stress is assumed to have many effects on menstruation of highschool senior female students, and it is expected to cause many problems and low efficiency during their studies. Therefore, through analyzing the menstruation state of highschool senior female students, the effects of stress on their menstruation and their education were studied. Methods: We made a survey of 583 senior female students of 'N' highschool located in Taegu. The survey was composed of the degrees of stress they were undergoing, menstrual problems caused by stress. Results: The survey results showed that 96.7% of the candidates were experiencing stress. 51.6% of them experienced an altered menstrual cycle, and menstrual irregularity was brought out in 32.1 % among them. 18.1 % of them were experiencing dysfunctional uterine bleeding. In sustaining period of menstruation, 17.5% of them had a shortened menstruation period, and 8.9% had a prolonged period. In case of menstrual quantity, less than normal quantity was reported by 26.9% of respondents and more than normal quantity was reported by 8.8%. 26.6% of respondents reported their menstrual blood was impure and the color was dark. Blood clots were increased in 27.5% of respondents. 86.6% went through premenstrual syndrome, and 34.3% had worsened menstrual pain. Studying was affected by menstrual pain in 83.9% of respondents. Of treating the disorder, 66.2% of them said they just waited till the pain went away, and 26.9% of them said they went to a pharmacy, and 2.4% of them said they went to an oriental medical clinic. Conclusions: From the above results, it is concluded that stress has a close connection with menstrual disorders. And it affects senior highschool female students' education, while they don't take proper treatments. Hence we think that we must have a lot of concern about senior female students and they must receive proper treatments.

Objectives: When stroke occurs, Korean people usually hope to be treated by traditional therapy and to be admitted to an oriental hospital. So this observation was done to make a small contribution toward stroke studies at oriental hospitals. Methods: This observation was made on 189 cases of stroke patients who were admitted to Kang Nam oriental medicine Hospital of Kyung Hee University from May, 1999 to August, 2000. Results and Conclusions : 1. The incidence of cerebral infarction was 3.6 times that of cerebral hemorrhage. 2. The incidence in females was 1.25 times of that in males. The incidence of stroke increased with aging and more cerebral hemorrhage occurred in lower age group than cerebral infarction. 3. There was higher morbidity in October and May, during the changing of the seasons, than any other months. 4. Hypertension the most common preceding disease, followed by heart disease, diabetes mellitus, hyperlipidemia. 5. Alcohol was the highest risk factor, followed by smoking, then obesity. 6. In cerebral infarction, MCA territory was the most frequent lesion site. 7. The change of Modefied Bathel Index(MBI) between MCA territory and others in cerebral infarction had no significance for 4weeks.(P>0.05)

Objectives : The purpose of this study was to examine the efficacy of Gamichunggan-tang(Jiaweiqinggan-tang) on 57 patients who have suffered from chronic liver disease. Methods : Gamichunggan-tang(Jiaweiqinggan-tang) was administered to patients for over 2 months continuously. We checked improvement of clinical symptoms, changes of Hepatitis B markers and lymphocyte count. Results : Gamichunggan-tang(Jiaweiqinggan-tang) has significant effect on the improvement of clinical symptoms. And the ratio of seroconversion from HBeAg positive to HBeAg negative was 42.9%. Lymphocyte increased in 83.3% of patients converted to HBeAg negative. Conclusions : It is suggested that Gamichunggan-tang(Jiaweiqinggan-tang) has significant effects on the recovery of weakened liver function and immune modulation. This treatment could be recommened as a prescription for Chronic liver disease.

Objectives : This study was undertaken to examine the clinical effect of ECM.E, made of hyaluronic acid, on dysmenorrhea. Methods : This study involved 44 women with dysmenorrhea. During the three-month study, 3T was administered to them twice a day and they were examined on their menstrual state three times. The degree of pain was measured by VAS. Conclusions : The followings are the conclusions from the study. 1. ECM.E yielded a significant effect on making the menstrual color healthy and reducing the amount of the menstrual clots. 2. ECM.E yielded a significant effect on reducing PMS, especially the mammary pain before menstruation. 3. ECM.E yielded a significant effect on reducing dysmenorrhea, especially the pain of lower abdomen, fatigue and abnormal excrements during menstruation.

Objectives: The aim of this study was to investigate the attitude toward the oriental hospital among 1,234 outpatients. Methods: A questionnaire about the general characteristics of patients, the decision-maker of use and the reason of choice or alteration was implemented. Results: The results of this survey as follows: Among outpatients 56.4 % were female in sex, 25.6 % were below 9 years of age; in educational level, 50.1 % were college graduates. Among outpatients, 33.6% chose the oriental hospital as their first choice and the rest were recommended by other persons. The recommenders were mostly relatives(73.9 %), female(73.4 %), young(30-39 years old, 40.1 %), high educational level (above the college, 54.9 %) people. 37.8 % among outpatients were secondary selection after the western medical clinic for the same symptom and sign. 7.0 % were the concomitant users of both therapies. 62.8 % among the concomitant users did not notify the western doctor about the dual therapies. The reasons they did not notify their Western doctors were 'be afraid of western doctor s bias' (42.6 %), 'no special reason' (29.6 %). Conclusions: The appropriate transfer system between oriental and western clinic must be constructed and the proper recognition about dual therapies will be needed among patients and doctors who practice both kinds of medicine.

Objectives and Method : In this study, I have investigated what kind of primary factors detennine the apoplexy prognosis, and the method of the function assessment about the apoplexy by inquiry into the literature on this subject Results and Conclusions : 1. The primary factors to have an effect upon the appoplexy are : the location of Pungsa; whether five organs ki is existent or not; pulse feeling; tongue condition; whether or not the patient produces stool or urine; for males, the left side; for females, the right side ; whether or not the patient sweats; consciousness; vital signs; and the region and size of disease and brain hernia. 2. MBI is often used because it is considered to be objective, simple, and highly reliable. But its absence of a legal recognition assessment is a major incongruence. 3. Inclusive and standard assessment are key points in the reinforecement by AM of legal recognition assessment, but it takes a lot of time and is not endowed with adding an extra weight and is vague to the division between the communication and social recognition grade. 4. AI is useful and easy to evaluate the mental ability, the capacity for locomotion and the daily activities inclusively.

Objective : The purpose of this study was to investigate the back shape of school children using the phase-shifting scanning grating projection moire interferometer, which was developed by the Korea Advanced Institute of Science and Technology and is useful in evaluating three dimensional back shape. Methods : In this study the subjects consisted of 1,358 pupils [711 boys(52.36%), 647 girls(47.64%)] attending elementary and middle schools in Seoul. Their ages ranged from nine to fifteen and the average age was 12.2. With the phase-shifting scanning grating projection moire interferometer, the posterior view of the body were taken to see if there are correlations of moire fringe number, width difference between left and right, and correlation between differences in moire fringe number and width on both sides in the scapular, lumbar and gluteal regions. Results : The results were as follows : I. More frequent findings of fringe were observed on the right in all regions : in the scapular region, 309 boys(43.4%) and 156 girls(24.2%) had more fringe numbers on the right side; in the lumbar region, 68 boys(9.5%) and 11 girls(1.7%); and in the gluteal region, 160 boys(22.4%) and 63 girls(9.8%). Such tendency was striking especially in the scapular and lumbar regions, and in boys rather than in girls. In the scapula, 661 subjects(48.7%) with one moire fringe on either side need further attention and 110 subjects(8.I %) with two or more are required to do follow-up radiography for scoliosis. 2. In an analysis of width difference in the trunk, the left side is wider in all regions except for the gluteal region in boys : in the scapular region 21 boys(3.0%) and 103 girls(15.9%); in the lumbar region, 87 boys(12.2%) and 250 girls(38.6%); and in the gluteal region 197 girls(30.4%) had a wider left side and 45 boys(6.3%) showed a wider right side. 3. In correlation analysis of the number of moire fringe and width difference in each region, the side where more moire fringes were observed was significantly wider in the lumbar and gluteal regions, but not in the scapular region.(p<0.01) Conclusions : From these results, it is concluded that the back shape of elementary and middle school students in Seoul shows that the right side had more moire fringes; the left side was wider; and especially in the lumbar and gluteal regions the side where more moire fringes were observed was wider.

Objectives : First, to restore reliability of oriental prescriptions that are effective against incurable diseases, Second, in the short term, to secure the validity of traditional remedies with the help of accumulated data and in the long term, develop new therapeutic methods and prescriptions in order to broaden its aspect in the field of medicine. Third, to maintain predominance on oriental therapeutic methods and put it into practical use based upon the results obtained by this paper. Methods : In the primary sub-thesis(herbalogical analytic methods applied on unknown oriental prescriptions), models of analytic method and in gathering information that could be put into practice are being revised In the secondary sub-thesis(method in gathering and analysing in dealing with oriental prescription), several statistical approaches and analysis on data that has been gathered are being revised. In the tertiary sub-thesis(research on legal guarantee of the offerer rights), an alternative scheme that covers the limitations of the legislative content in dealing with offerer rights is being revised Results : This research has revealed several problems, including those which were foreseen, in proceeding with the project. The prospect of the involvement of the medical personnel engaging in the field of oriental medicine was remarkably bright. Given the condition that the project will proceed as it follows, unknown oriental prescriptions and remedies which have been particularly ignored will eventually play an important role in clinical practice. Conclusions : It is clear to everyone that these oriental remedies will remain ignored by the public unless they gain popularity. Strict verifications on these oriental remedies are definitely needed in order to overcome this limitation. Finally, it should provide a momentum in the field of medicine in gaining popularity to the public.

Objectives : In order to apply herbal medicine to aroma-therapy, we observed clinically remedical effect for allergic rhinitis of aroma-therapy using herbal medicine. Methods : Twenty-four allergic rhinitis patients were treated in the Kun-po Oriental Medical Hospital of Wonkwang University from September 2000 to October 2000. They were treated with aroma-therapy and analyzed clinically. Results : 1. 25.0% (6 cases) of all cases involved pre-teenagers; teenagers 29.2% (7 cases); patients in their 20s, 20.8% (5 cases); patients in their 30s, 12.5% (3 cases); patients in their 40s, 8.3% (2 cases); patients in their 50s, 4.2% (1 cases). 2. The ratio of male to female patients was 58.3%(14 cases) to 41.7%(10 cases). 3. Cases involving patients under the age of six months was 0%; under 1 year 12.5% (3 cases); under 2 years 33.3%(8 cases); under 3 years 16.7%(4 cases); over 3 years 37.5%(9 cases). 4. Past history : The cases of sinusitis were 33.3%(8 cases); otitis media 20.8%(5 cases), bronchitis 16.7%(4 cases), atopic dermatitis 12.5%(3 cases), asthma 8.3%(2 cases). 5. The ratio of allergic onset based on seasons were : spring 16.7%(4 cases), summer 0%, fall 29.2%(7 cases), winter 16.7%(4 cases), and not defined 37.5%(9 cases). 6. The cases of nasal obstruction was 91.7%(22 cases); followed by : sneezing 83.3(20 cases), white rhinorrhea 70.8%(17 cases), eye itching 66.7%(16 cases), nasal itching 58.3%(14 cases), rhinalgia 58.3%(14 cases), postnasal drip 50.0%(12 cases), headache 41.7%(10 cases), yellow rhinorrhea 33.3%(8 cases), cough 33.3%(8 cases), fatigue 29.2%(7 cases). 7. The cases of familial factor was 66.7%(16 cases); the non-familial factor 33.3%(8 cases). 8. The remedical effect of the treatment showed an improvement in 58.4% of the total cases studied. 9. The improvement-rate of the 1st group (mahwang group) was 66.7%; of the 2nd group (hwangkum group) 48.5%, and of the 3rd group (pine group) 55.8%. Conclusions : Considering the above results, we have concluded that the remedical effect for allergic rhinitis of aroma-therapy using herbal medicine showed to be intentional in comparison with aroma-therapy using aroma-oil.

Objectives : The present study was carried out to investigate the effects of Danchun-hwan(DCH) on the peroxynitrite-induced neural cell death in human neuroblastoma cell line, SH-SY5Y. Methods : The cultured cells were pretreated with DCH and exposed to 3-morpholinosydnonimine(SIN-1) that simultaneously generates NO and superoxide, thus possibly forming peroxynitrite. The cell damage was assessed by using MTT assay and crystal violet staining. Results : Exposure of the cells to SIN-1 for 24hr induced 75% apoptotic cell death, as evaluated by the occurrence of morphological nuclear changes characteristic of apoptosis using 4', 6-diamidino-2-phenylinole(DAPI). However, pretreatment of SH-SY5Y with the water extracts of DCH, inhibited the apoptotic cell death in a dose-dependent manner. DCH also inhibited SIN-1-induced apoptotic caspase 3-like protease activity in a dose-dependent manner. DCH recovered the depleted glutathione levels by SIN-1. Conclusions : Taken together, it is suggested that DCH protected human neuroblastoma cell line, SH-SY5Y, from the free radical injury mediated by peroxynitrite by a mechanism of elevating antioxidant, GSH.

Objectives : Hindered barrier function of vascular endothelium has been implicated in the initiation and progression of degenerative vascular diseases such as atherosclerosis. In this study, the effect of Sunghyangchungisan(SHCS) as a protectant against oxidant-induced destruction of endothelial barrier function was assessed. Methods : Toward this end, endothelial cells derived from the human umbilical vein were cultured as monolayers on permeable membrane filters. Endothelial permeability was monitored by measuring transendothelial electrical resistance and movement of low density lipoprotein (LDL) across the endothelial monolayer. Results : Along with increased movement of LDL, $H_2O_2$-induced increase in endothelial permeability was paralleled by a decrease in transendotheliaI electrical resistance. The effect of $H_2O_2$ was mimicked by phorbol 12-myristate 13-acetate (PMA), a potent activator of proteinkinase C. Calphostin-C, a protein kinase C inhibitor, effectively blocked the increase in endothelial permeability induced by $H_2O_2$ or PMA, indicating that activation of protein kinase C is associated with the $H_2O_2-induced$ permeability change. SHCS effectively protected the endothelial monolayer against $H_2O_2-induced$ increase in permeability, whereas, it did not affect PMA-induced change. Forskolin, a potent activator of adenylyl cyclase, antagonized $H_2O_2$ to increase endothelial permeability. In addition, in ${H_2O_2}-treated$ cens, intracenular cAMP concentration was significantly decreased, indicating that impaired cAMP production as well as activation of proteinkinase C is a mechanism underlying ${H_2O_2}>-induced$$H_2O_2$ with regard to its effect on intracellular cAMP content. However, SHCS itself did not affect resting cAMP concentration in endothelial cells. Conclusions : These results suggest that SHCS might operate as an effective protectant against oxidant-induced destruction of endothelial barrier function. The mechanism does not appear to involve direct interaction with protein kinase C- or cAMP-associated signaling mechanism.

Objectives: This study was conducted to establish the study form of atopic dermatitis in oriental medicine, to find out the effect of herbal medicine and to compare Bangpungtongsungsan group with symptom-complexes prescription group. Methods: We gave scores(1-3) to the 37 outpatients who visited the dept. of Ophthalmotolaryngology & Dermatosurgery of the Kyunghee Oriental Medical Center from June 1,2000 to September 30, 2000. According to transformed the G. Rajka, T. Langeland and Jon M. Hanifin standard. We checked the Eosinophil count, IgE and the scores (intensity: the degree of itching, course: the degree of itching during a year, extent: erythema, lichenification, scaling. dryness, erosion' oozing). After herbal treatment for 6 weeks, we checked the above factors again in the same way. In treatment, we divided the patients into Bangpungtongsungsan group and Symptom-complexes group for cross-comparison. Results: There were 19 patients we could check by score and 14 patients by laboratory. 1. In the 14 lab cases, the number of abnormal value of Eosinophil count and IgE was decreased but it was not significant. 2. In the 19 cases by score, clinical severity of the intensity of itching, lichenification, scaling' dryness was significantly decreased between the before- and after-herbal treatment periods (P<0.01, P<0.01, P<0.05). 3. In 19 cases, clinical severity of erythema, erosion. oozing was decreased after herbal treatment but it was not significant(p>0.05). 4. In point of total score, clinical severity of atopic dermatitis was significantly decreased after herbal treatment(p<0.01). 5. There was no significant difference between Bangpungtongsungsan group and Symptom-complexes group(P<0.05). Conclusions: Although more study is needed, these results indicate that herbal treatment is effective on atopic dermatitis.

Objectives : The aim of this clinical experiment was to investigate the effect of KamiWooseul-tang on low back pain and kidney function. Methods: We investigated 64 low back pain patients. We treated the patients with acupuncture, bed rest, herbal medicine, physical therapy. The patients were divided into two groups as follows, 32 patients (with normal renal function) were treated with only KamiWooseul-tang. Another 32 patients were treated with a commonly used herbal medicine. Results : The obtained results are summarized as follows. The patients were 43.8% male and 56.2% female. Their age were 20s 15.6%, 30s 25%, 40s 17.2%, 50s 9.3%, 60s 18.8% 70s 12.5% and 80s1.6%. Duration is most common in the most acute stage (< 1 week), next was chronic stage ( >6 months ), acute stage (1 week ~ 1 month) and subacute stage(l months ~ 6 months) in turn. Cure rate increased step by step after herb-medicine administration. Duration of patients had no relation with cure rate(P>0.05). The KamiWooseul-tang was more effective than the commonly used herbal medicine after 3 weeks therapy (P<0.05). Serum BUN and Creatinine level in KamiWooseul-tang group changed from $14.69{\pm}4.0,{\;}0.76{\pm}0.19(mg/dl)$ before adminstration to $13.64{\pm}3.92{\;}0.77{\pm}0.19,{\;}13.48{\pm}3.00{\;}0.82{\pm}0.21,{\;}13.26{\pm}3.73{\;}0.87{\pm}0.21(mg/dl)$ at 7th, 14th, and 21st days of administration respectively. Urinalysis showed no specific change in the KamiWooseul-tang group. During medication serum-electrolyte was within normal range. Conclusion : Therefore KamiWooseul-tang was more effective than the commonly used herbal medicine and there was no clinically remarkable difference in the serum BUN, Creatinine level, urinalysis and serum electrolyte between pre-medication and post-medication in the KamiWooseul-tang group.

Background and Purpose : Medical and Neurological complications in patients with acute stroke may affect their prognosis including death rate, function and admission period. Some of these complications may be preventable. But few data exist regarding complications occurring in the first weeks. So we sought to observe the type, timing, and frequency of complications in hospitalized patients after stroke. Methods : We retrospectively examined the case notes of patients admitted in Won Kwang Oriental Medical Hospital after stroke. Two observers inspected the case notes using predifined diagnostic criteria and recorded the type, timing, and frequency of complications that occurred during the inpatient period. Results : Complications were recorded in 43 patients(82.7%). The most common medical complications were constipation(25.0%) and shoulder pain(21.2%). The most frequent serious medical problems were pulmonary infection(9.6%) and UTI(7.7%). The most common neurological complications were insomnia(34.6%) and dysphagia(23.7%). The most frequent serious medical problem was mental deterioration(7.7%). Conclusion : Complications after acute stroke are common. There were more medical complications than neurological complications. So we should compile much knowledge about medical complications and treat them actively. The differences between our study and previous studies are attributable to the different methods including patient selection and diagnostic criteria.

Background and Purpose : Cerebrovascular accident is a serious neurologic event. It can have temporary or permanent effects on survivors, including memory, cognition and volitional control of voiding. The purpose of this study is to evaluate the clinical effect of moxibustion in patients with voiding dysfunction after a cerebrovascular accident. Methods : Twenty patients with post-stroke voiding dysfunction were studied. All patients had computerized tomography(CT) and magnetic resonance imaging(MRI) to localize the lesion in the central nervous system. They were randomly divided into two groups : the control and moxibustion group. Ten of twenty patients underwent moxibustion treatment by randomization. The moxibustion group receieved moxibustion at three points : Chung-guk (Conception Vessel CV3), Kuanwon (CV4) Kihae (CV6). Residual urine volume evaluation was undertaken in all patients. Results : 1. IIn the balanced bladder time, the moxibustion group had the shorter time than non moxibustion group. But there was no significiant difference between the moxibustion group and non-moxibustion group. 2. In the residual urine volume, the moxibustion group and non-moxibustion group showed a tendency to decrease. But there was no significiant difference between the moxibustion group and non-moxibustion group. Conclusions : Though further study is needed, our findings suggested that the time of achieving balanced voiding was shorter with moxibustion than in the control group.

Tingling, pins-and-needles, numbness, burning and raw sensations are the words frequently used by patients to describe such conditions as paresthesia and obstruction syndrome of Ki and blood, Obstruction syndrome of Ki and blood results from the complex elements of wind, cold and dampness and has a process of stagnating Ki, blood and meridian system, The subject was a male patient who had obstructive syndrome of Ki and blood, We administered the medication with dispeling the pathogenic factor, adjusting the constructive and defensive energy, tonifying the Ki and blood by stage. The subject reported reduced pain, an increased range of motion and improved hyperlipidemia.

The subject was a 70-year-old man who was suffering from productive cough after stroke. His symptoms were night cough, thick sputum production, reddened tongue and night sweat. The subject was diagnosed as having a deficiency of Yin, and was treated with Chungsangboha-tang(Qingshangbuxia-tang). A decrease in the severity of the subject's cough and sputum were observed, and $O_2$ saturation increased. In this case Chungsangboha-tang(Qingshangbuxia-tang) was effective on the patient with night cough, thick sputum production, reddened tongue and night sweat due to deficiency of Yin after stroke.

The subject was a 46-year-old woman who was suffering from severe vertigo. Her major symptoms were vertigo, headache, neck pain, feverishness, and her minor symptoms were intermittent nausea and tinnitus, mild chest discomfort, facial flushing and sweating. Her symptoms were diagnosed as Chi-Vertigo and the patient was treated with Boheo-eum(Buxu-yin). Next, the patient's vertigo was evaluated with Vestibular disorders activities of Daily Living scale and Dix-Hallpike maneuver per 3days. An improved state in vestibular disorders activities of daily living scale, a frequency and duration of Dix-Hallpike maneuver were observed. In this case Boheo-eum(Buxu-yin) has an effect on a patient with vertigo due to Chi-Vertigo.

This case study was performed to investigate the effects of Chongsimyonja-tang and Dong's acupuncture therapy on senile macular degeneration patient who has been treated in the Dong-Eui oriental hospital. A 57-year-old female with senile macular degeneration complained of headaches, heaviness of the ache, night sweat, dizziness. For a duration of 2 months, We prescribed Chongsimyonja-tang, and added 3-4 herbs according to the symtoms and also treated the patient with Dong s acupuncture therapy. After 2 months, the patient's eyesight improved ($0.2{\rightarrow}0.7$) and the headaches disappeared, as did heaviness of the ache, night sweat, and dizziness. In this case Chongsimyonja-tang and Dong's acupuncture therapy present valuable treatment of senile macular degeneration.

The occurrence of neurologic symptoms after cervical manipulation(Chuna treatment) seems to be an uncommon event considering the large number of manipulations performed. The precise incidence is unknown, for only patients with serious complications seek medical care. We recently experienced a 3D-year-old woman who developed sudden neurologic complications after cervical Chuna treatment by a nonprofessional therapist. Whether the manipulation is the major causative factor in the subsequent stroke can be disputable and must be considered in each case. Physicians and patients, however, should be aware that cerebrovascular accidents may occur as a result of cervical manipulation.

A growing number of people are concerned about their sleep. There are many people with chronic sleep disorders. As there are various causes in a disease, proper treatment according to each cause is necessary for a more effective treatment. In general, insomnia is classified into five categories of physical, physiological, psychological, psychiatric and pharmacological aspects. Sedative-hypnotics including benzodiazepine and non-benzodiazepine have widely been used in chronic insomniacs. However, most hypnotics including non-benzodiazepine cause some of dependence, tolerance, impaired daytime function and rebound insomnia. Therefore, we are looking forward to proposing an effective oriental treatment for insomnia. A 71-year-old male who had suffered from cerebral infarction was admitted to our department for oriental treatment of stroke and insomnia. Initial treatment modalities with administration of paroxetine were not effective. However administration of oriental medicine' Gochimmuwoo-san(Gaozhenwuyou-san)' achieved a desirable effect.

Paraquat is a very potent herbicide which causes fatal toxicity when ingested, and there is no specific antidote against it. Human ingestion induces acute renal failure, hepatic dysfunction and progressive respiratory failure with high mortality rate. Clinical investigation and medical treatment were done on two cases of acute renal failure caused by paraquat poisoning admitted to the Department of Internal Medicine, Wonkwang University Oriental Chonju Medical Hospital. We report two cases of patients who survived after acute paraquat intoxication, by means of oriental medicine such as Gamdutang, a typical antidote of toxins, chinese ink as an absorbent and burned powder of Rhei Radix et Rhizoma for laxative and so on, western medicine such as gastric lavage, diuretics and fluid therapy. We suggest more experiments and studies related to such treatment for paraquat poisoning be conducted.

Behcet's disease is a systemic disease affecting multiple organs including the central nervous system. Neuro-Behcet's disease was regarded as relatively rare, but thanks to the development of diagnostic tools, more and more cases are being reported. We are reporting a case of neuro-Behcet's disease in which the patient displayed paraparesis, dysarthria and involuntary tremor as neurologic symptoms. The patient's brain MRI showed cerebellar atrophy, and a spinal cord MRI failed to reveal any significant lesions. The patient experienced a couple of fever attacks during hospitalization, which were managed adequately by herbal medicines. Her main neurological symptoms such as paraparesis were, however, grossly unchanged at discharge.

We experienced two cases of Tsutsugamushi disease which occurred in October, 2000. The patients, who were over 70 years old and living in a rural area, visited Wonkwang University Oriental Chonju Medicine Hospital because of suffering fever, chill, skin rash, abdominal discomfort, and general weakness for a duration of seven days. The diagnosis was confmned as Tsutsugamushi disease by clinical findings such as eschar and high antibody titers on Reverse Passive Hemagglutination(RPHA). As for treatment, we used Sengmagalgentang-gamibang during the acute and Chojungikitang during convalescent phases of the illness. The patients were treated with Doxycycline of western medicine. The patients improved within 3 days of admission and were discharged within one week.