This study aims to propose a lighting design guide by investigating the lighting metrics of circadian rhythm for proper human-centric lighting in architectural environments. A systematic review of previous studies was examined based on experiments with biosignal responses under various lighting levels. The results of this analysis showed that the lighting metrics of circadian rhythm were color rendering index (CRI), gamut area index (GAI), correlated color temperature (CCT), illuminance, circadian stimulus (CS), equivalent melanopic lx (EML), and monochromatic light. Biosignal responses were melatonin, cortisol, alpha, beta, theta, eye blinking, core body temperature, and blood pressure used to measure circadian rhythm, visual fatigue, and eye fatigue. The melatonin level of circadian rhythm was higher for the lighting with higher EML than for the lighting with lower EML. Visual fatigue was higher for the lighting with higher CCT, illuminance, CS, and EML than for the lighting with lower CCT, illuminance, CS, and EML. Eye fatigue was higher in the lighting with higher CIE CRI than for the lighting with lower CIE CRI. Therefore, these findings suggest that proper human-centric lighting design should consider a four-step process that includes characterizing the lighting application and determining the lighting levels for the melatonin level of circadian rhythm of users, visual fatigue of users, and eye fatigue of users.