Objective: The purpose of this study was to analyze the causal relationship between parenting knowledge, parenting stress, and parenting behavior of mothers with infants and to confirm the longitudinal mediating effect of parenting stress in the relationship between parenting knowledge and parenting behavior. Methods: This study used data collected through the Panel Study on Korean Children from 2008 to 2010 and the participants were 1,444 mothers with infants. The data were analyzed through technical statistics, correlations and multivariate potential growth models using SPSS 28.0 and AMOS 26.0. Results: Parenting knowledge and parenting stress of mothers gradually increased, and positive parenting behavior gradually decreased. Having a lot of parenting knowledge reduced mother's parenting stress. Mother's parenting stress reduced positive parenting behavior, and as parenting stress increased, positive parenting behavior decreased significantly. However, the relationship between parenting knowledge and parenting behavior was not significant. In addition, the mediating effect of parenting stress was confirmed in the relationship between parenting knowledge and parenting behavior of mothers. Conclusion/Implications: The results of this study suggest the need to reduce parenting stress and promote positive parenting behavior along with the systematization of parent education programs in order for mothers with infants to acquire parenting knowledge.