Objective: This study aims to provide empirical data on epidemic management measures and childcare teacher education in centers. It examines the relationship between infectious disease knowledge, health control knowledge, and self-efficacy in relation to the practice of preventive behaviors among infant and toddler teachers during the COVID-19 pandemic. Methods: The study involved 300 teachers from Seoul and Gyeonggi Province. Data were collected through questionnaires and analyzed using SPSS 23.0. Results: The study found that there is a positive relationship between childcare teachers' knowledge of infectious diseases and their engagement in preventive behavior. Additionally, internal control among the health care factors of childcare teachers also showed a positive relationship. Furthermore, the study revealed that the self-efficacy of childcare teachers is positively associated with their practice of preventive behavior. Overall, infectious disease knowledge, internal control measures, and self-efficacy were all found to have positive relationships with preventive behavior. Conclusion/Implications: This study proposes a new direction for future teacher education by highlighting the effectiveness of psychological factors, specifically health control materials and self-efficacy, in addition to focusing on improving infectious disease knowledge.