Objective: The aim of this study is to analyze the previously published research articles related to puffer fish toxin focusing on tumor. Method: Literatures were searched in PubMed database, published since 2000, using the keyword; Puffer fish, Fugu and tetrodotoxin (TTX) with cancer or tumor. Research papers were classified by year, country, study model, used material, kind of tumor and study subject. Finally, a total of 41 studies were analyzed in this study. Results: From 2000 to 2018, the most abundant papers were published in 2009 (6 studies) and almost half of the papers were studied in United Kingdom (20 studies). The 39 studies used TTX purified from puffer fish while 2 studies used crude extract of skin and gonad of puffer fish. The most used target cell line was prostate cancer (15 studies), and the next was breast cancer (14 studies). The study methods were classified into 4 clinical studies, 2 animal studies and 35 cell-based studies. Conclusions: Our results show that the overview of cancer-related studies using puffer fish poison. This information would be helpful for the puffer fish-derived drug researches in the future.

Objectives : The aim of this review was to analyse the study tendency in papers related with Ssanghwa-tang which are published in Korea from 2000 to 2019. Methods : We searched the four electronic database (NSDL, RISS, Korean traditional knowledge portal, OASIS) and checked relevant Korean journals from 2000 to 2019. We classified the papers by publish date, speciality, study method, and field of study, and analysed the study tendency. Result : 1. papers were published annually on average. 2. After classifying papers by the speciality of journal, continuous study was followed not only in korean medicine, but also in many specialities like Dietetics, Pharmacology and Tourism management. 3. In study methods, in vivo was 46%, quantitative inspection was 21%, and in vitro was 19%. 4. After classifying papers by field of study, efficacy was 49%, qualitative analysis was 27%, toxicity was 14%, and safety was 5%. 5. In efficacy, it was effective in muscloskeletal disease, cardiovascular disease, prevention of neurologic disease, antioxidation, immunization disease and so on. Conclusions : These results suggest that Ssanghwa-tang can be used as cure medicine, not just as herbal tonic, but there are not sufficient evidence based papers, so there should be further studies in order to establish Ssanghwa-tang as a cure medicine.

Objectives : Hwangryunhaedok-tang. a traditional Korean herbal medicine, has been widely used for the treatment. This study is purposed to analyze the research trend of Hwangryunhaedok-tang in Korea for developing further research plan. Methods : A search of Korean research database( OASIS, RISS, Korean traditional knowledge portal, NDSL) was conducted to find studies for Hwangryunhaedok-tang. We analyzed studies by publish date, speciality, study method, and field of study. Result : 1. 2.65 papers were published annually on average. 2. After analyzing for the speciality of the journal, most of the papers were published in Traditional Korean Medicine. 3. After analyzing for the study method, in vivo was 33.96%, in vitro was 32.08% and quantitative inspection was 13.21% 4. After analyzing for the subject of the studies, beneficial effect was 81.13%, quantitative and qualitative analysis was 13.21%, toxicity was 3.77% 5. Papers on beneficial effect were classified by the type of diseases. Hwangryunhaedok-tang was most used in skin disease. Conclusions : Hwangryunhaedok-tang is effective in various category of diseases. This study will be useful to future researchers and clinicians to conduct a study on Hwangryunhaedok-tang.

Objective & Method : We investigated the identity of chilsonparik which is written in Hwangjenaekyung and claimed by traditional scholars, and drew the following conclusions. Result & Conclusion : The word "Chilsonparik"(七損八益) is written in Hwangjenaekyung(黃帝內經). However the exact meaning of "Chilsonparik" doesn't appear in the Hwangjenaekyung so several scholars were studying to find out its true meaning. In 1973, a book "Cheonhajidodam"(天下至道談) was unearthed in the mawangtoe Han Tomb in Hunan province. This book "Cheonhajidodam" had a detailed description of "Chilsonparik", and its contents were similar to those of the "Hwangjenaekyung". In this sense, the sentences of "Hwangjenaekyung" is likely to have copied the contents of "Cheonhajidodam" or have quoted the same book, which makes it possible to interpret "Chilsonparik" in the "Hwangjenaekyung" relying on the description of "Chilsonparik" from the "Cheonhajidodam". Based on "Cheonhajidodam" the "Chilson" refers to "Seven ways of sexual intercourse that bring harm to the human body" and "Parik" refers to "Eight ways of sexual intercourse that bring benefit to the human body". The description of "Chilsonparik" in "Cheonhajidodam" is way more specific than the interpretation of the posterity scholars of the later generations. It is a also seen that the contents of the "Cheonhajidodam is appropriate as the "Hwangjenaekyung" depicts the "Chilsonparik" in concrete acts and ways. It is thought that the explanation of "Cheonhajidodam" gave correct answers to the "Chilsonparik" that was left as a question for us.

Objectives : Atopic dermatitis is an irritable skin disease accompanying rash and itching leading to impaired skin barrier. ATO-ALL is an ethanol extract of natural products comprising 12 herbs and effective on atopic dermatitis. In this study, we aimed to propose that the effect of ATO-ALL on skin regeneration in human keratinocyte cell line, HaCaT cells. Methods : To evaluate the skin regenerating effects of ATO-ALL, scratch wound healing assay, bromodeoxyuridine (BrdU) assay, and propidum iodide (PI) assay were performed using cultured HaCaT cell line. Result : Scratch wound healing assay showed that ATO-ALL was able to enhance the gap filling activity more than 2-fold at 7 ppm concentration compared with control group. BrdU assay demonstrated that ATO-ALL treatment increased the de novo cell proliferation in a dose-dependent manner. Finally, PI assay indicated that the cell cycle of HaCaT cells was modulated by ATO-ALL treatment. Conclusions : These results suggested that ATO-ALL may have skin regenerating effects by increasing cell proliferation via cell cycle regulation. Taken together, ATO-ALL is supposed to have a potential on regeneration of damaged skin or functional disease including atopic dermatitis.

Objectives : The aim of this study was to observe the clinical effect of warming acupunture therapy at Buyang(BL59), Golyun(BL60) in a traffic accident patient with neck pain. Methods : A 32-years-old, female patient with neck pain was treated by warming acupunture therapy at Buyang(BL59), Golyun(BL60) from April 28th to May 5th. The improvement of the patient's neck pain was evaluated by Visual Analog Scale(VAS), Pain Rating Score(PRS) and Neck Disability Index(NDI). Result : After treatment, flexion & extension VAS was decreased from 7.0 to 3.0 and lateral bending VAS was decreased from 7.5 to 4.0. PRS and NDI was improved 70 to 54, 41 to 35, respectively. Conclusions : The result suggests that warming acupunture therapy at Buyang(BL59), Golyun(BL60) may have therapeutic effect on neck pain patients.

Objectives : The purpose of this case study is to report the effect of Korean medicine on hand-foot syndrome(HFS) casued by sorafenib(nexavar) in hepatocellular carcinoma. Methods : The patient, 77-year-old patient complaining with pain, swelling and erythema on both hands and feet which is characterized as HFS was treated with Jaungo. We evaluated the symptoms by Numeric Rating Scale and pictures. Results : The symptoms of HFS has been alleviated during the treatment. Conclusions : We suggest Jaungo as one of the alternative therapies for HFS due to sorafenib.