Purpose: The purpose of this study was to identify the effects of the gender role conflict on nursing professionalism in male nursing college students. Method: The subjects were 113 male college students in D and J city. The data were collected from April 24 to May 3, in 2017 using a structured questionnaire. The data were analyzed using the SPSS WIN 24.0 program. For data analysis, independent t-test, One-way ANOVA, Pearson's correlation coefficient and multiple linear regression analysis were carried out. Results: The results are follows. First, the individual characteristics in gender role conflict were significantly different in motivation(F=2.62, p=0.028) and the entering college decision(F=3.14, p=0.028), and nursing professionalism were significantly different in the entering college decision(F=4.34, p=0.006). Second, a negative relationship was observed between the gender role conflict and nursing professionalism(r=-.287, p=<0.01). The success, power and competition(${\beta}=0.19$, p<0.05) and male superiority(${\beta}=-0.47$, p<0.001) were found to influence nursing professionalism. Conclusion: These results highlight the need to develop educational intervention to lower the superiority of the male and to encourager success, power and competition of male nursing college students.