Purpose. The purpose of this study was to investigate the effect of changes in tear break-up time, accommodative response amount, and accommodative microwave on the eyes due to changes in humidity. Methods. A total of 48 adult men and women in their 20s (21.1±1.56 years old) were surveyed on dry eye questionnaires (OSID questionnaires), tear destruction time was measured by humidity (40%, 60%, 80%), and controlled reactions and controlled fine waves were measured through tactile tests. Result. As a result of the OSID questionnaire, there were 10 normal patients, 8 mild dry eyes, 1 moderate dry eye, and 5 severe dry eyes. The initial tear film destruction time was 7.34±4.67 seconds at 40% humidity, 8.99±4.75 seconds at 60% humidity, 10.26±4.52 seconds at 80% humidity, and the average tear film destruction time was 9.51±4.07 seconds at 40% humidity, 10.99±3.82 seconds at 60% humidity, and 12.05±3.42 seconds at 80% humidity. The accommodative response was -0.41~1.79D at 40% humidity, -0.34~1.79D at 60% humidity, and -0.30~2.01D at 80% humidity. The accommodative microwave was measured as 56.39~63.43dB when it was 40%, 56.41~62.64dB when it was 60%, and 55.96 ~61.13dB when it was 80%. Conclusion. As the humidity (40~80%) increased, the tear break-up time increased, and as the accommodative stimulus amount increased, the accommodative response amount increased. It was found that the accommodative microwave according to the amount of accommodative stimulation increased, and as the humidity increased, the accommodative microwave decreased.