Purpose: The purpose of this study was to investigate the factors affecting the prevention behavior of COVID-19 infection among nursing students. Methods: This survey was conducted from September 3 to October 2, 2021 among 172 sophomore and senior students at universities in D metropolitan city and J province. The data were analyzed using descriptive statistics, t-test, ANOVA, Pearson's correlation and Hierarchical Regression. Results: There were significant differences in COVID-19 preventive behavior by gender (t=0.13, p=.017) and clinical practice experience (t=2.78, p=.006). COVID-19 preventive behavior showed significantly positive correlations with subjective norm (r=.35, p<.001), self-efficacy(r=.33, p<.001), and intention(r=.62, p<.001). The variables affecting COVID-19 preventive behavior were intention(β=.529, p<.001), subjective norm(β=.169, p=.008), and clinical practice experience(β=.118, p=.049), and the explanatory power was 43%. Conclusion: Considering these results, it is necessary to develop strategies that can encourage the intention of nursing students to practice prevention for COVID-19 infection control. In addition, maintaining subjective norms through national policies is a critical strategy.