Purpose: The purpose of this study was to examine the relationships of attitudes, stigma, and moral sensitivity of nurses toward HIV/AIDS. Methods: The participants were 530 nurses working in general hospitals in South Korea. A structured questionnaire regarding attitudes, stigma toward HIV/AIDS, and moral sensitivity was used. The data were analyzed using t-tests, ANOVAs, and Pearson correlation coefficients. Results: Nurses' attitudes toward HIV/AIDS differed by job position; nurses' moral sensitivity toward HIV/AIDS differed by age, marital status, education level, clinical practice career, and job position; and nurses' stigma toward HIV/AIDS differed by age, clinical practice career, subjective economic status, HIV/AIDS education experience, and HIV/AIDS patient care experience. Attitudes of nurses toward HIV/AIDS were more negative when stigma toward HIV/AIDS was higher (r=-0.58, p<.001), these attitudes were not correlated with moral sensitivity. Conclusions: The attitudes of nurses toward HIV/AIDS were more negative when stigma toward HIV was higher, and these attitudes were not correlated with moral sensitivity. Therefore, nurses should be provided education that takes their age, marital status, clinical practice career, and experiences in HIV-related education and caregiving into account.