Purpose: Wogonin is an active component isolated from scutellariae radix. This study was conducted to evaluate the inhibitory effect of wogonin mixed with hydrogel on osteoclast differentiation. Methods: It was performed to estimate cytotoxicity of Wogonin alginate hydrogel disk(WHD) in BMMs stimulated with RANKL, M-CSF. ROS synthesis and actin ring formation were analysed to observe the effect of WHD. Results: WHD has no cytotoxicity at the concentration of 0.1 ${\mu}g/ml$ or lower. 0.1 ${\mu}g/ml$, 1 ${\mu}g/ml$ WHD restrained the synthesis of ROS and 0.1 ${\mu}g/ml$, 1 ${\mu}g/ml$ WHD restrained the formation of actin ring. Conclusions: WHD has the inhibitory effect of osteoclast differentiation and bone resorption. Further studies are needed to treat osteoporosis by herbal medicine.

Objectives: This study was performed to elucidate the effects of Kwibi-tang extract(KB) on dermal fibroblast. Methods: To demonstrate the effects of KB on dermal fibroblast, we used human dermal fibroblast(F6) and UVB light(30 $mJ/cm^2$) was used to damage to dermal fibroblast. we measured the nitrite production, LDH release in UVB-irradiated dermal fibroblast to elucidate the action-mechanism of KB. Also, we evaluated cell proliferation of dermal fibroblast and the amount of increased PICP, TIMP-1 in dermal fibroblast. Results: 1. KB decreased the cell proliferation of F6 dermal fibroblast in concentration of 50 ${\mu}g/ml$. 2. KB decreased the synthesis of PICP in concentration of 50 ${\mu}g/ml$. 3. KB decreased the synthesis of TIMP-1 in concentration of 50 ${\mu}g/ml$. 4. KB have no effect on the damage in UVB-irradiated F6 dermal fibroblast. Conclusions: From the results, we concluded KB decreases the cell proliferation and collagen synthesis in dermal fibroblast. So we suggest that KB has the anti-hyperplasy of dermal fibroblast.

Purpose: Polycystic ovary syndrome(PCOS) is a common condition characterised by menstrual abnormalities. PCOS is now recognised to be a metabolic syndrome which may include hyperinsulinemia, hyperlipidemia, diabetes mellitus and possibly cardiac disease. Cyperi Rhizoma(CR) can soothe the liver to regulate gi and regulate menstruation to relieve pain. For these reasons, this study was designed to investigate the effects of CR on metabolic syndrome in PCO rats. Methods: Female rats injected with a single dose of 2 mg/kg estradiol valerate (EV) develop PCO, CR was given for 5 weeks. After 5 weeks administration of drugs, body weights, food uptake, Fasting Blood Glucose, Oral Glucose Tolerance Test, Total Cholesterol, HDL-Cholesterol, LDL-Cholesterol and Triglyceride were measured once a week. Results: In this experiment, single injection of EV induced hyperlipidemia, but it did not affect serum glucose levels. Oral administration of CR prevent weight loss shown in control group. In addition, treatment with CR can lower total cholesterol, LDL-cholesterol and triglyceride in serum compared to PCO induced control group. Conclusions: These results suggest that CR can regulate hyperlipidemia in PCOS.

Objectives: This study was performed to assess the protective effect of Polygonum multiflorum(PM) on UVB-irradiated HaCaT Keratinocytes damage. Methods: The protective effects of Polygonum multiflorum(PM) were determined by UVB-irradiated HaCaT assay. We assessed protective effects of Polygonum multiflorum(PM) on LDH release and nitrite production from HaCaT. COX-2, Bcl-2, Bax, $TNF{\alpha}$, c-jun, c-fos, NF-${\kappa}B$, iNOS, Bcl-xL gene expression were determined in HaCaT using real-time PCR method. Results: 1. PM inhibited LDH Release in UVB-irradiated HaCaT Keratinocytes. 2. PM inhibited Nitrite Production in UVB-irradiated HaCaT Keratinocytes. 3. PM suppressed the Gene Expression of COX-2 in UVB-irradiated HaCaT Keratinocytes. 4. PM increased the Gene Expression of Bcl-2 in UVB-irradiated HaCaT Keratinocytes. 5. PM didn't increase the Gene Expression of Bax in UVB-irradiated HaCaT Keratinocytes. 6. PM suppressed the Gene Expression of $TNF{\alpha}$ in UVB-irradiated HaCaT Keratinocytes. 7. PM suppressed the Gene Expression of c-jun in UVB-irradiated HaCaT Keratinocytes. 8. PM suppressed the Gene Expression of c-fos in UVB-irradiated HaCaT Keratinocytes. 9. PM suppressed the Gene Expression of NF-${\kappa}B$ in UVB-irradiated HaCaT Keratinocytes. 10. PM suppressed the Gene Expression of i-NOS in UVB-irradiated HaCaT Keratinocytes. 11. PM didn't increase the Gene Expression of Bcl-xL in UVB-irradiated HaCaT Keratinocytes Conclusions: In conclusion, these results suggest that PM inhibited the cell damage in UVB-irradiated HaCaT.

Purpose: This study was performed to analyse autonomic nerve function of some examinees with dysmenorrhea by comparing the characteristics of dysmenorrhea patients' Heart Rate Variability(HRV) in examinees group with non-examinees group. Methods: We studied 110 patients visiting OO Korean hospital to take medical treatment for dysmenorrhea from September, 2006 to August, 2010. The subjects were categorized in two groups, 36 examinees and 57 non-examinees controls. We investigated the difference of HRV between two groups by student's T-test using SPSS for Windows(version 12.0). Results: 1. SDNN in examinees group is statistical significantly lower than non-examinees group. RMS-SD in examinees group is lower than non-examinees group non-statistical significantly. Mean PR in examinees group is higher than non-examinees group non-statistical significantly. 2. VLF, ln TP, ln VLF and ln HF are statistically significantly lower than non-examinees group. TP, HF and ln LF are lower than non-examinees group non-statistical significantly. LF is higher than non-examinees group non-statistical significantly. Conclusions: The results suggest that stress is related to especially examinees in dysmenorrhea patients by effecting autonomic nerve function. HRV can be useful to diagnose examinees' dysmenorrhea. The further studies would be needed to study about relation between stress, dysmenorrhea and HRV for settling this.

Objectives: The purpose of this study is to investigate the relationship between cold hypersensitivity and common health indexes including dyspepsia, headache, defecation, leucorrhea, and menstruation in young women. Methods: 40 outpatients who visited Kangnam Kyung-Hee Oriental Hospital from January, 2010 to November, 2011 were analyzed. The experimental group consisted of 20 patients diagnosed with cold hypersensitivity, while the control group consisted of 20 patients who were not. All patients were subjected to Digital Infrared Thermal Imaging(DITI), and those with thermal difference greater than $2^{\circ}C$ between anterior thigh and top of feet were diagnosed with cold hypersensitivity. The subjective characteristics of dyspepsia, headache, defecation, leucorrhea, and menstruation were obtained through inquiry by residents at the hospital. The relationship between cold hypersensitivity, dyspepsia, headache, defecation, leucorrhea, and menstruation was analyzed with Independent T-test using SPSS for Windows (version 17.0). Results: Means of defecation, leucorrhea, dysmenorrhea in cold hypersensitivity group were higher than control group without significant statistical difference. Dyspepsia appeared statistically higher in experimental group with significance while headache was the same in both groups. Means of menstrual cycle and menstrual coagulation were higher in non-cold hypersensitivity group, but there was no statistical difference. Conclusions: Results suggest that dyspepsia is common in cold hypersensitivity and treatment of cold hypersensitivity may relieve accompanying digestive problems.

Purpose: To evaluate the correlation of MRS, MENQOL, HRV, Yin-Deficiency Questionnaire and the clinical use of Yin-Deficiency Questionnaire by comparison with MRS, MENQOL, and HRV. Methods: The participants were 21 women(44-58yr) with hot flush who were not currently on hormone therapy. The evaluating index of this trial are MRS, MENQOL, HRV, Yin-Deficiency Questionnaire. Results: The MRS and the MENQOL were significantly correlated with each other. The MRS and the HRV(LF/HF ratio) were significantly correlated with each other. The MENQOL and the HRV(LF/HF ratio) were significantly correlated with each other. The MRS somatic subscale and the Yin-Deficiency Questionnaire were significantly correlated with each other. The MENQOL and the Yin-Deficiency Questionnaire were significantly correlated with each other. The Yin-Deficiency Questionnaire and the HRV(LF/HF ratio) were significantly correlated with each other. Conclusions: The Yin-Deficiency Questionnaire is a valuable tool of oriental medicine for assessment of symptoms. The Yin-Deficiency Questionnaire were significantly correlated with the MRS somatic subscale, the MENQOL and the HRV(LF/HF ratio). Yin-Deficiency Questionnaire could serve as an adequate diagnostic instrument of oriental medicine for menopausal syndrome. Further large-scale study is needed for evaluation of the correlation of these measurements related with Menopausal syndrome and the clinical use of Yin-Deficiency Questionnaire.

Purpose: Hominis placenta have used widely for women's disease like climacteric syndrome, dysmenorrhea, infertility, but we don't have enough evidence with it. This study is to investigate efficacy and safety of Hominis Placenta on women by investigating papers. Methods: We searched for papers which had Hominis placenta, placental extract associated with women's disease in the Pubmed and Korean journals. Results: 20 papers were found. 15 papers were associated with menopausal disorder. Hominis placenta could have the efficacy on osteoporosis and climacteric syndrome induced by menopause. And each 2 papers were relevant to postpartum symptoms and menses. 1 papers was relevant to pregnancy. Most of studies have shown that Hominis placenta is useful for female disorders. Conclusions: Hominis placenta could be a good treatment for female disorder like climacteric syndrome, dysmenorrhea, infertility. But evidence is not enough with Hominis placenta, so more research will be needed.

Objectives: The purpose of this study is to investigate the relationship between the degree of menopausal symptom and granulocyte/lymphocyte ratio, and heart rate varibility(HRV). Methods: From 11th April 2011 to 20th May 2011, a total 30 female subjects (age 41-60) was recruited. We evaluated the degree of menopausal symptoms by Kupperman' s index and measured subject's WBC differential count and HRV. The data were analyzed by Kruskal-Wallis test and Spearman correlation test using SPSS/PC ver 13.0 program. Results: 1. There was no statistically significant difference in granulocyte/lymphocyte ratio and HRV among 4 classified groups according to Kupperman's index.- Mild, Moderate, Severe and Urgent. 2. Granulocyte ratio and general symptom score(within Kupperman's index) significantly showed negative correlation coefficient(r=-0.499, p=0.005), and Lymphocyte ratio and general symptom score significantly showed positive correlation coefficient (r=0.374, p=0.042). 3. Granulocyte/lymphocyte ratio and HRV showed no significant correlation. Conclusions: There was no significant relation among the degree of menopausal symptoms and granulocyte/lymphocyte ratio, and HRV. But furthermore large scale study is required.

Objectives: To examine the current status of clinical research in oriental medicine, and to assess 'risk of bias'(ROB) of randomized clinical trials(RCTs) in oriental medicine in Korea. Methods: Special committee for EBM, KOMS(Korean Oriental Medicine Society) reviewed 17 journals related to oriental medicine in Korea (from the first issue to May 2010), the Cochrane Central Register of Controlled Trials (The Cochrane Library Issue 2, 2010), and PubMed (1966 to May 2010). Then we selected eligible RCTs in terms of oriental medicine, and assessed 'risk of bias'. Results: We reviewed 12,653 articles from the 17 journals, and 41 articles from CENTRAL and PubMed. After non-clinical articles were excluded, 1,004 articles were left. Among them, the number of eligible studies in terms of oriental medicine was 306. In these eligible studies, 130 were RCTs. Of RCTs, 69 were on acupuncture, 25 on herbal medicine. The proportion of 'unclear' is high in the criteria of 'Allocation concealment', 'Blinding of participants and personnel', 'Blinding of outcome assessment' and 'Other bias'. On the other hand, 'low' has high in the criteria of 'Incomplete outcome data' and 'Selective reporting'. Conclusions: Risk of bias on oriental medicine is unclear in terms of 'allocation concealment' and 'blinding'. For high-quality research in oriental medicine, further research should be needed on randomization and blinding in the RCTs.

Objectives: To understand relations general characteristics, lifestyle habits including smoking, alcohol habit, exercise, eating habit and menopausal symptoms measurement indicators during treatment for hot flush in menopausal women. Methods: The participants were 159 women (45~60 yr) who were not currently on hormone therapy, and had reached hot flash scores of 10 or higher. The evaluating indexes of this trial are hot flush score, hot flush visual analogue scale(VAS), Hot flush consistence time, sweating visual analogue scale(VAS), Menopause Rating Scale(MRS), Menopause-specific quality of life questionnaire(MENQOL), Kupperman's Index. Results: 1. When the participants divided according to body mass index(BMI), overweight group showed a high score in all indicators. Therefore, obesity can aggravate the symptoms of menopause. 2. When the participants divided according to drinking habits, Hot flush consistence time of non-alcohol group showed a statistically significant difference. However, the sample size is uneven. Additional studies will be needed. 3. When the participants divided according to eating habits, Hot flush consistence time of regular group showed a statistically significant difference. However, the sample size uneven. Additional studies will be needed. 4. When the participants was divided depending on whether exercise, non-exercise group showed a high score in all indicators. Thus, exercise will be able to improve menopausal symptoms. Conclusions: Improvement of obesity and exercise will be able to improve menopausal symptoms.

Purpose: KCDO-3(Korean Classification of Diseases(Oriental Medicine)-third edition) being used in January, 2010 accepted the KCD(Korean Classification of Diseases) and added disease pattern and syndrome of oriental medicine. But, the diagnoses of oriental medicine are too uncertain to express in A00-Z99(KCD). In this case, you should choose in U codes under the KCD use guidelines, but U codes are not capable of representing the symptoms too. So, we suggest the use criteria and consider the weakness of the U codes with medical records of patients who visited with amenorrhea or oligomenorrhea. Methods: We referred medical records of patients who visited oriental obstetrics and gynecology from January 1st to December 31st, 2010. From among them, we set up 122 patients who related with emmeniopathy as target group and searched codes distribution based on medical records. And we described that the process of choosing appropriate codes based on the medical records of 49 amenorrhea or oligomenorrhea patients. Results and Conclusions: The emmeniopathy is divided into menstrual disorder, amenorrhea and systemic disorders at the period of menstruation. And emmeniopathy is expressed in some codes such as N91, N92, N93, N94, U321, U77. When a patient visit hospital, a doctor should choose causal codes when there is confirmed diagnosis. Otherwise, a doctor chooses symptom codes. And if there are more than two diagnosis consistent with definition of chief condition, a doctor should code the first listed diagnosis as a chief condition. Because KCD-5 is classified according to western medical diagnosis, it is difficult to choose in KCD-5 when we diagnosed with disease pattern and syndrome of oriental medicine. But U codes are also deficient to express various condition of emmeniopathy. So we should add 'deficiency and detriment of the thoroughfare and conception vessels', 'prolonged menstruation' and various systemic disorders at the period of menstruation.

Purpose: To investigate the change of the climacteric syndrome with women with induced menopause by hysterectomy or ovariectomy compared with women with natural menopause. Methods: Menopausal women(aged 45-55) were selected at two oriental medicine hospitals, Sam-se Oriental Medicine Hospital and Daegu Hannny Oriental Medicine Hospital. They were asked about Kupperman's Index, MENQOL and a few additional questions. They were divided into two groups: women with induced menopause by hysterectomy or ovariectomy and women with natural menopause. To analyse the Kupperman's index and MENQOL, SPSS for Windows 12.0 and Wilcoxon Ranked Sum Test were used. Results: The total sums of Kupperman's index and MENQOL were significantly correlated with women with induced menopause and women with natural menopause and women with induced menopause had higher total sums. Both the renal and urinary tract disorder and the sexual disorder were significantly correlated with women with induced menopause and women with natural menopause. The gastrointestinal and psychosocial disorder of Kupperman's index and MENQOL were not significantly correlated with women with induced menopause and women with natural menopause. Conclusions: Women with induced menopause by hysterectomy or ovariectomy experienced climacteric syndrome more severely compared with women with natural menopause, specifically the renal and urinary tract disorder and sexual disorder.

Purpose: To show the clinical aspects in outpatients who visited Oriental Obstetric & Gynecology Clinic Methods: We studied 7141 patients who visited Oriental Obstetric & Gynecology Clinic from 2008 to 2010. Results: 1. The total patients and the percentage of new patients increased every year. 2. The age distribution showed over 60% in 20~49. 3. The percentage of Oriental Ob&Gy patients decreased with 55% in 2008, 28% in 2009, 29% in 2010. 4. The distribution of oriental diagnosis showed 55.4% in Ob&Gy, 19.2% in musculoskeletal system, 6% digestive system and respiratory system neuropsychiatry in 2008. And 30.6% in musculoskeletal system, 28.5% in Ob&Gy, 10.9% neuropsychiatry in 2009. And 29% in musculoskeletal system, 28.1% in Ob&Gy, 11.1% in heart system in 2010. 5. The most commonly used herbal medicine was Ikgibohyeltang. Conclusions: We hope these results could be helpful to diagnosis and treatment of oriental Ob&Gy patients.

Objectives: The purpose of this study is to investigate all postpartum care center in Seoul about its state of doctors' linkage and oriental medical treatment. Methods: From May 1st 2011 to July 10th 2011, survey was done using a selfmade questionnaire by phone or visit. There were 12 categories including the state of doctors' linkage in postpartum care centers, treatment form of oriental medical doctors, utilization of herbal medicine and so on. The data collected was analyzed using Excel 2007 FOR WINDOWS. Results: 1. The doctors' linkage with postpartum centers included pediatricians(96.1%), oriental medicine doctors(61.2%), and gynecologists(34.0%). Of those postpartum centers, 66% had both pediatrician and oriental medical doctor which were the vast majority. 2. Out of 70 postpartum centers providing herbal medicine, 9 of them were supplied of herbal medicine by herb sellers(not doctor). Where as, at 63 oriental medical doctors-affiliated centers, 85.7% of them had oriental medical doctors' examinations. 3. Out of 70 postpartum centers providing herbal medicine, personalized prescription amounted to 35(50.0%), same prescriptions amounted to 34(48.6%), and 1(1.4%) refused to answer. 4. Out of all the patients that received explanation about the effects of herbal medicine, 62.9% had them explained by oriental medical doctors. Conclusions: These findings suggests that oriental medical doctors are actively involved with postpartum care centers but problems surfaced regarding individualized prescription insufficiency and the lack of administration. In the near future, if the expertise expand in the areas of pediatrics as well as obstetrics and gynecology, oriental medicine will have a more solid role in postpartum care part.

Purpose: This study was performed to find correlations between Edema Index and Heart Rate Variability(HRV) conducted in early postpartum period. Methods: 17 postpartum women were participated in this study which was conducted with Inbody 720(Biospace Co. Ltd.) and SA-6000(Medicore Co. Ltd.). Edema Index(Extra Cellular Water/Total Body Water) was calculated after body composition analysis and HRV result(TP, VLF, LF, HF, HF norm, LF norm, LF/HF ratio) was analysed within a week after delivery. Results: There was no significant correlations between Edema Index and HRV results in time domain analysis. also Edema Index and TP, LF, HF, LF/HF ratio have no significant correlations in frequency domain analysis. but only VLF data has significance compared with Edema Index. Conclusions: It can be suggested that VLF results analysed by HRV can be useful reference estimating postpartum edema especially occurs in early postpartum period.

Purpose: Many infertile women are receiving in vitro fertilization-embryo transfer (IVF-ET). But side effects occur after IVF-ET and pregnancy rate is still low. So this study is to report the effect of herbal medicine on a woman receiving IVF-ET and herbal medicine's low risk on a pregnant woman. Methods: The patient was underwent artificial insemination five times and IVF-ET three times. In this study, the patient steadily took a herbal medicine before IVF-ET to birth. Results: The side effects of IVF-ET and the miscarriage symptoms ware decreased. And by taking a herbal medicine during IVF-ET, the patient was pregnant and gave birth when she was 33 weeks pregnant by cesarian section. Conclusions: This case study shows that herbal medicine is effective for reducing side effects of IVF-ET and increasing pregnancy rate. And in this case the patient gave birth to healthy babies, althogh steadily taking herbal medicine during pregnancy.

Objectives: This study was to report the effect of Hyeolbuchukyeo-tang(HC) on complications of post-hysterectomy. Methods: Three women who were undertaken a hysterectomy within a month were enrolled in this study. They complained of abdominal pain, lumbago, bladder pain, tinnitus, both leg pain, headache, etc. And received oriental treatments to decrease pain and improve the symptoms. They took Hyeolbuchukyeo-tang 3 times a day. Results: A hysterectomy can lead to complications. They complained of various symptoms. After the treatment, abdominal pain, bladder pain, both leg pain, headache were rapidly decreased and lumbago, tinnitus were improved too. Conclusions: These clinical cases indicated that Hyeolbuchukyeo-tang was effective in the treatment of women who undertaken a hysterectomy.