Purpose: This study was conducted to evaluate the inhibitory effect of Achyranthis Radix extract(ARE) loaded hydrogel on osteoclast differentiation. Methods: MTT-assay was performed to estimate cytotoxicity of ARE, Achyranthis Radix-alginate hydrogel disk(ARHD) in bone marrow macrophages stimulated(BMMs) with human receptor activator of nuclear factor-${\kappa}B$ ligand(RANKL), human macrophage-colony stimulating factor(M-CSF). Tartrate resistant acid phosphatase staining and RT-PCR were performed to know the inhibitory effect on osteoclast differentiation. Reactive oxygen species and actin ring formation were analysed to observe the effect of ARHD. Results: ARE has no cytotoxicity at the concentration of 0.1 $mg/m{\ell}$ or lower. ARE decreased the number of TRAP positive cells in RANKL, M-CSF stimulated BMMs and the gene expression. ARHD has no cytotoxicity at the concentration of 10 ${\mu}g/m{\ell}$ (24, 48hours), 50 ${\mu}g/m{\ell}$ (24 hours). ARHD restrained the synthesis of reactive oxygen species and the formation of actin ring. Conclusions: Achyranthis Radix has the inhibitory effect of osteoclast differentiation and bone resorption. Further studies are needed to treat osteoporosis by Achyranthis Radix.

Purpose: The purpose of this study is to evaluate the synergic efficacy of oriental herb medicine for osteoporosis patients by examining whether there is any change in BMD. Osteoporosis patients are classified into a group having taken Fosamax and the other group having taken Fasamax and Samgieumgamibang in this study. Methods: Mean BMD and T-score are measured in all patients before and 3 months after treatment. Results: 1. The group having taken Fosamax showed no statistically significant mean BMD change before and after treatment. 2. The group having taken Fosamax showed no statistically significant T-score change before and after treatment. 3. The group having taken both Fosamax and Samgieumgamibang showed increase in mean BMD, which does not bear statistically significant change. 4. The group having taken both Fosamax and Samgieumgamibang showed statistically significant T-score change before and after treatment(p<0.05). Conclusions: After treatment, BMD in patients having taken Fosamx did not change significantly, but in patients having taken both Fosamax and Samgieumgamibang increased, which proves the efficacy of oriental herb medicine for osteoporosis patients.

Objectives: This study was performed to elucidate the effects of Kanghwalsokdan-tang extract(KS) on hyper-plasy of collagen and cell damage in UVB-irradiated dermal fibroblast. Methods: To demonstrate the effects of KS on wound healing we used human dermal fibroblast(F6). We evaluated the amount of increased PICP, TIMP-1 in dermal fibroblast. PICP, TIMP-1 concentration was measured using EIA kit. Also, we measured the nitrite production, and LDH release in UVB-irradiated dermal fibroblast to elucidate the action-mechanism of KS. Results: 1. KS decreased the cell proliferation of dermal fibroblast. 2. KS decreased the biosynthesis of collagen in dermal fibroblast. 3. KS decreased the synthesis of TIMP-1 in dermal fibroblast. 4. KS had no effect on the LDH-release of UVB-irradiated dermal fibroblast. 5. KS inhibited nitrite production in UVB-irradiated dermal fibroblast. Conclusions: From the results, we concluded that KS has a protective effect on wound healing and photoaging.

Objectives: The object of this study was to observe the in vitro antibacterial effects of Wandae-tang extracts and combination of Wandae-tang extracts and Clindamycin against Gardnerella vaginalis ATCC14018. Methods: Antibacterial activities against Gardnerella vaginalis ATCC14018 of Wandae-tang extracts were detected using standard agar microdilution methods. In addition, the effects on the bacterial growth curve were also monitored at minimal inhibitory concentration(MIC) and $MIC{\times}2$ levels. The combination effects of Wandae-tang extracts with Clindamycin were observed by Checkerboard microtiter assay, and the effects of bacterial growth curve was treated with Wandae-tang extracts MIC+Clindamycin MIC, 1/2 MIC and 1/4 MIC, respectively. Results: MIC of Wandae-tang extracts and Clindamycin against Gardnerella vaginalis ATCC14018 were detected as $1.719{\pm}0.856$(0.782~3.125) $mg/m{\ell}$ and $0.010{\pm}0.006$ (0.004~0.016) ${\mu}g/m{\ell}$. In addition, Clindamycin and Wandae-tang extracts were also showed marked dosage-dependent inhibition of bacterial growth, and more dramatical inhibitions were detected in Clindamycin+Wandae-tang extracts MIC treatment. Fractional inhibitory concentration index in combination of Wandae-tang extracts and Clindamycin were detected as $0.294{\pm}0.052$(0.250~0.375) at Checkerboard microtiter assay. Conclusions: The results obtained in this study suggest that Wandae-tang extracts showed antibacterial effects against Gardnerella vaginalis ATCC14018, and they also showed dosage-dependent inhibitory effects on the bacterial growth. In addition, combination treatment of Wandae-tang extracts with Clindamycin showed more synergistically potent inhibitory effects on the growth of Gardnerella vaginalis.

Purpose: Dysmenorrhea mostly depends on the causative factor, which usually falls under the categories of Deficiency and Excess pattern in traditional Oriental medical theories and diagnosis. Thus, we investigated menstruation symptom and sign related to dysmenorrhea and verified the validity of Deficiency and Excess pattern identification. Methods: We investigated menstruation symptom and sign related to dysmenorrhea in total 14 gynecology-medical books including the book ${\ll}$Exemplar Of Korean Medicine (Dongui Bogam)${\gg}$ and whole body symptom and sign identifying Deficiency and Excess pattern at the same time. A survey based on this investigation was carried out targeting women of childbearing age. Results: Total of 14 gynecology-medical books have mostly narrated pre-menstrual and mid & post-menstrual pelvic pain depending on the time of its manifestation for identifying Deficiency and Excess pattern. Dysmenorrhea in pre-menstrual period belonged to Excess pattern and dysmenorrhea in mid & post-menstrual period belonged to Deficiency pattern. Among a total of 343 women, 196 subjects suffered from dysmenorrhea. The number of dysmenorrhea in pre-menstrual period (Excess pattern) was 116 people and in mid & post-menstrual period (Deficiency pattern) was 80 people. Deficiency and Excess pattern of dysmenorrhea in menstrual period significantly correlated to Deficiency and Excess pattern of whole body symptom and sign in the statistics(P-value < 0.05). Conclusion: The results suggest that pre-menstrual and mid & post-menstrual pelvic pain depending on the time of its manifestation is preferentially utilized as symptom and sign related to dysmenorrhea identifying Deficiency and Excess pattern.

Objectives: This study was conducted to investigate the relationship between menstrual conditions and items of health checkup examinations in female university students for prediction of reproductive health. Methods: Data from the 2011's medical examination participants(990 students among total 1,699 students) of ${\bigcirc}{\bigcirc}$ University University in Korea were analyzed for this study. Menstrual condition, Sa-sang constitution and other surveys were checked through self-administered questionnaire, health checkup were measured anthropometric variables, CBC, B/C and U/A. Results: In the 46.4% of total participants appeared menstrual disorder of menstrual cycle or menstrual amount, the irregular period of menstrual cycle have a influence on menstrual amount. stress was relatively heavy factor of causing irregularity of menstrual cycle and amount, RBC, cholesterol, ABO type and Sa-sang constitution had some connection with menstrual amount. Conclusions: Among the items of health checkup examinations, stress, RBC, cholesterol, ABO type and Sa-sang constitution were related with the change of menstrual cycle and amount.

Objectives: The purpose of this article is not until to investigate the changes and types of statistical methods and to point out the statistical errors after analyzing the method of articles that improve the quality of the statistical analysis of papers published in the Journal of Oriental Obstetrics and Gynecology. Methods: Papers published in the Journal of Oriental Obstetrics and Gynecology from 2009 to 2011 were reviewed for methodological and statistical validity using a modified version of Ahn's checklist. A statistician reviewed individual papers and evaluated the list items in the checklist for each paper. To avoid the potential assessment error by the statistician who lacks expertise in the field of Oriental Obstetrics and Gynecology. Results: A total of 190 papers including 64 original articles, 40 reviews article, 58 case report and 28 brief communication were reviewed. Statistics methods used in 121 papers were composed of t-test(58.7%), ANOVA test(19.8%) and ${\chi}^2$- test (14.0%) et al. Whereas only 14.9% of papers were free of statistical errors, the number of omission errors was 58 and the number of commission errors was 149 each. Conclusions: A variety of statistical errors were encountered in papers published in the Journal of Oriental Obstetrics and Gynecology. Accordingly, researchers should be more careful when it comes to describing and applying statistical methods.

Purpose: Obesity affects dysfunction of Hypothalamus-Pituitary-Ovary(H-P-O) axis. Dysfunction of H-P-O axis could be related to Skin Resistance Variability (SRV). This study investigated the impacts of obesity which related to female age on SRV. Methods: We randomly selected 400 women who had taken CP-6000A test and InBody test on the same day at Oriental Hospital of Sangji University from Jan. 2008 to Jun. 2011. In this study, the Body Mass Index(BMI) range of normal group is 18.5~24.9, obesity group is over 25, the age range of childbearing age group is 24~34 years, post-menopause group is 50~65 years. 139 women that met the criteria of this study were assorted by their BMI and age. After detection of SRV, SPSS 19.0 has conjugated for data analysis and the independent samples t-test and paired samples t-test were used to verify the results. Results: At 1st and 2nd trial, the height of 3 area of childbearing age obesity group was significantly higher than that of childbearing age normal group. At 1st and 2nd trial, the height of 1, 2 area of childbearing age obesity group was higher than that of childbearing age normal group, but there were no significant difference. At 1st and 2nd trial, the height of 1, 2, 3 area of post-menopause obesity group was mostly higher than that of post-menopause normal group, but there were no significant difference. Conclusions: This study showed the SRV of obesity group was higher than that of normal group in most of 1, 2, 3 area. Further study will be needed.

Objectives: This study explored the relationships between Bone Mineral Density (BMD), Body Mass Index(BMI), Age, pain duration outcomes of treatment for Low Back Pain(LBP) in 3 groups(normal,osteopenia,osteoporosis) of female patients. Methods: We evaluated 32 cases of normal, 30 cases of osteopenia and 38 cases of osteoporosis based on BMD, BMI, Age, pain duration among the female inpatients with LBP caused by lumbar disc herniation, admitted at Jaseng Hospital of Oriental Medicine from January 2010 to December 2011. And we measured VNRS(Verbal Numeric Rating Scale) to evaluate the conservative treatment effects. Results: In improvement of female LBP caused by lumbar disc herniation, BMD(T-score), BMI had not proved correlation(P-value>0.05). Female patients' age was negatively correlated with T-score. In pain improvement, BMD normal group showed more reduction of NRS(numeric rating scale) than osteoporosis group, but that had not proved correlation(p-value=0.893). Conclusions: Statically, among the female inpatients with LBP caused by lumbar disc herniation, had no significant differences between the normal group, osteopenia group and osteoporosis group's improvement rate, depending on treating period.

Purpose: The purpose of this study is to report the clinical effectiveness of oriental medicine on overactive bladder. Methods: The patients suffering from overactive bladder were treated by oriental medical treatment such as herbal medication, acupuncture and moxibustion therapy. Results: After Oriental medical treatments, the symptoms of overactive bladder disappeared and the patients' quality of life improved. Conclusions: This case report shows that the oriental medical therapy is effective against overactive bladder.

Objectives: Pregnant women often experience the symptoms of Hyperemesis Gravidarium. Many pregnant women think it is a passing symptom, so, they usually don't receive aggressive treatment. But if there is a heavy degree may lead to dangerous situations, so, if necessary, should receive aggressive treatment. Methods: The treatment of Hyperemesis Gravidarium, there are several ways. We provided herbal medicine and acupuncture, observed changes of subjective complaint. Results: In this report, we gained significant effects with oriental medical treatment. To this Hyperemesis Gravidarium patient, oriental medical treatment rather than conservative treatment using fluid therapy and antiemetics was effective. Conclusions: This patient was mainly treated with herbal medicine and acupuncture of oriental medical treatment. But, to the patient of Hyperemesis Gravidarium, you may consider other treatments and we suggest that you should take advantage of various therapies.