• Molecules and Cells
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• v.44 no.1
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• pp.38-49
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• 2021

Airway mucus secretion is an essential innate immune response for host protection. However, overproduction and hypersecretion of mucus, mainly composed of the gel-forming MUC5AC protein, are significant risk factors for patients with asthma and chronic obstructive pulmonary disease (COPD). The transforming growth factor β (TGFβ) signaling pathway negatively regulates MUC5AC expression; however, the underlying molecular mechanism is not fully understood. Here, we showed that TGFβ significantly reduces the expression of MUC5AC mRNA and its protein in NCI-H292 cells, a human mucoepidermoid carcinoma cell line. This reduced MUC5AC expression was restored by a TGFβ receptor inhibitor (SB431542), but not by the inhibition of NF-κB (BAY11-7082 or Triptolide) or PI3K (LY294002) activities. TGFβ-activated Smad3 dose-dependently bound to MUC5AC promoter. Notably, TGFβ-activated Smad3 recruited HDAC2 and facilitated nuclear translocation of HDAC2, thereby inducing the deacetylation of NF-κB at K310, which is essential for a reduction in NF-κB transcriptional activity. Both TGFβ-induced nuclear translocation of Smad3/HDAC2 and deacetylation of NF-κB at K310 were suppressed by a Smad3 inhibitor (SIS3). These results suggest that the TGFβ-activated Smad3/HDAC2 complex is an essential negative regulator for MUC5AC expression and an epigenetic regulator for NF-κB acetylation. Therefore, these results collectively suggest that modulation of the TGFβ1/Smad3/HDAC2/NF-κB pathway axis can be a promising way to improve lung function as a treatment strategy for asthma and COPD.

• Journal of Ginseng Research
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• v.45 no.1
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• pp.134-148
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• 2021

Background: Lung cancer has a high incidence worldwide, and most lung cancer-associated deaths are attributable to cancer metastasis. Although several medicinal properties of Panax ginseng Meyer have been reported, the effect of ginsenosides Rk1 and Rg5 on epithelial-mesenchymal transition (EMT) stimulated by transforming growth factor beta 1 (TGF-β1) and self-renewal in A549 cells is relatively unknown. Methods: We treated TGF-β1 or alternatively Rk1 and Rg5 in A549 cells. We used western blot analysis, real-time polymerase chain reaction (qPCR), wound healing assay, Matrigel invasion assay, and anoikis assays to determine the effect of Rk1 and Rg5 on TGF-mediated EMT in lung cancer cell. In addition, we performed tumorsphere formation assays and real-time PCR to evaluate the stem-like properties. Results: EMT is induced by TGF-β1 in A549 cells causing the development of cancer stem-like features. Expression of E-cadherin, an epithelial marker, decreased and an increase in vimentin expression was noted. Cell mobility, invasiveness, and anoikis resistance were enhanced with TGF-β1 treatment. In addition, the expression of stem cell markers, CD44, and CD133, was also increased. Treatment with Rk1 and Rg5 suppressed EMT by TGF-β1 and the development of stemness in a dose-dependent manner. Additionally, Rk1 and Rg5 markedly suppressed TGF-β1-induced metalloproteinase-2/9 (MMP2/9) activity, and activation of Smad2/3 and nuclear factor kappa B/extra-cellular signal regulated kinases (NF-kB/ERK) pathways in lung cancer cells. Conclusions: Rk1 and Rg5 regulate the EMT inducing TGF-β1 by suppressing the Smad and NF-κB/ERK pathways (non-Smad pathway).

• East Asian mathematical journal
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• v.17 no.1
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• pp.129-133
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• 2001

The aim of this research note is to derive the well known Kummer's second theorem by transforming the integrals which represent some generalized hypergeometric functions. This theorem can also be shown by combining two known Bailey's and Preece's identities for the product of generalized hypergeometric series.

• Water Engineering Research
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• v.5 no.4
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• pp.177-183
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• 2004

This paper introduces an eigenvalue method of transforming the hyperbolic partial differential equations of a particular unsteady friction water hammer model into characteristic form. This method is based on the solution of the corresponding one-dimensional Riemann problem that transforms hyperbolic quasi-linear equations into ordinary differential equations along the characteristic directions, which in this case arises as the eigenvalues of the system. A mathematical justification and generalization of the eigenvalues method is provided and this approach is compared to the traditional characteristic method.

• Proceedings of the Korean Society for Quality Management Conference
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• 1998.11a
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• pp.239-250
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• 1998

A method of finding optimal linear restriction on regression parameters in linear model for mixture experiments in the sense of minimizing integrated mean squared error is studied. We use the formulation of optimal restrictions on regression parameters for estimating responses proposed by Park(1981) by transforming mixture components to mathematically independent variables.

• Clinical and Experimental Pediatrics
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• v.53 no.7
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• pp.735-740
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• 2010

Renal fibrosis, characterized by tubulointerstitial fibrosis and glomerulosclerosis, is the final manifestation of chronic kidney disease. Renal fibrosis is characterized by an excessive accumulation and deposition of extracellular matrix components. This pathologic result usually originates from both underlying complicated cellular activities such as epithelial-to-mesenchymal transition, fibroblast activation, monocyte/macrophage infiltration, and cellular apoptosis and the activation of signaling molecules such as transforming growth factor beta and angiotensin II. However, because the pathogenesis of renal fibrosis is extremely complicated and our knowledge regarding this condition is still limited, further studies are needed.

• Journal of the Ergonomics Society of Korea
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• v.12 no.2
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• pp.29-43
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• 1993

This paper presents a conceptual approach to convert customer requirements expressed in ordinary language into a form of qualitative and quantitative functions for developing new products. This approach attempts to combine the concepts of the value engineering and the Kansei engineering. It emphasizes that customer require- ments should be interpreted and reflected on the design of new product. Specific are discussed for extracting subjective requirements and transforming them into qualitative and quantitative functions for product design. This approach is expected to provide the product designer with a systematic efficient tool for incorporating subjective requirements into a product design.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2002.03a
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• pp.143-150
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• 2002

This paper presents the application of continuous wavelet transform for determination of dynamic parameters of continuous beam subjected to moving load. Morlet wavelet is used as mother wavelet in wavelet transform. Dynamic parameters are estimated from the magnitudes and arguments of the wavelet coefficients obtained by wavelet transforming the response time histories of joints on the beam. This study shows that the estimated parameters such as natural frequencies, dmping ratios and mode shapes are to be well-compared with those of the finite element analysis.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.29B no.2
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• pp.9-17
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• 1992

In recent years, object-orientation is rising to notice as a new paradigm for developing software. This paper suggests the diagramming technique and a tool for developing C++ program effectively. This technique can represent the modularity and the interactions of classes definitely by emphasizing the characteristics of classes of C++. It can do the direct mapping from the logical idea to the physical screen image, so programmers can reuse the design resources in design phase as well as transforming the resources into chode in the implementation phase.

• Communications for Statistical Applications and Methods
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• v.5 no.2
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• pp.297-306
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• 1998

We consider a graphical method for visualizing the strictly monotonic transformation of t(y) so that the regression function E(t(y)$\mid$x) is linear in the predictor vector x. Cook and Weisberg (1994) proposed an inverse response plot which relies on the results of Li & Duan (1989) to obtain consistent estimates. Based on the recursive addition of the results from the two dimensional plots, we propose a new procedure which can be used when the consistency result is in doubt.