• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.482-487
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• 2009

The apple rootstock M.26 (Malus pumila) is very popular apple rootstock with semi-dwarf habit and the trees on M.26 produce high quality fruit at a young age. Since it is prone to poor prop ability to soil, however, young trees require staking in windy locations. The rolC gene was introduced into M.26 by Agrobacterium tumefaciens LBA4404 harboring pBI121 to obtain its transformants with dwarfism and enhanced rooting ability. One regenerated transgenic line was confirmed by polymerase chain reaction (PCR) analysis and Southern blot analysis of genomic DNA for the existence of rolC gene. The characteristics of transgenic line in vitro were not significantly different from non-transgenic line except for the active root formation and lateral root number. The rolC transgenic line showed reduced stem length and increased root number in vitro. Rooting ability was examined in the isolated greenhouse after mound layering. Compared to non-transgenic M.26, rolC transgenic line showed significantly higher rooting ability. The transgenic line did not show any other observable variation in shoot phenotype compared with non-transgenic line excepting increased branching

• Korean Journal of Environmental Agriculture
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• v.42 no.3
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• pp.239-252
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• 2023

Most apple trees in South Korea are grafted on M.9 and M.26 rootstocks; however, these rootstocks are susceptible to fire blight. Although M.7 rootstocks are moderately resistant to fire blight, they tend to exhibit excessive vigor, which is unsuitable for high-density planting, unless weak cultivars are used. This study investigated the vegetative growth, yield, and fruit quality of apple trees grafted onto M.7, M.9, or M.26 rootstocks to assess the feasibility of establishing high-density apple orchards domestically using the M.7 rootstock a period of seven years (1-7 years after planting). Rootstocks were tested using three cultivars with contrasting induced vigor and harvesting times: vigorous and late-maturing 'Fuji,' moderate vigor and middle-maturing 'Hongro,' and low vigor and early-maturing 'Sansa.' The planting density was maintained constant, with 190 trees per 10 a. Primary thinning (leaving only the king fruit on clusters) was performed, whereas secondary thinning (controlling crop load) was not. Vegetative growth, accumulated yield per 10 a, and yield efficiency varied depending on cultivars and rootstocks; however, the cultivars had a more notable effect on fruit quality than the rootstocks. Biennial bearing often occurred in the M.26 rootstock. 'Fuji'/M.7 was overly vigorous for high-density planting. The fruit quality and accumulated yield per 10 a of M.7 were similar to those of M.9 with the 'Hongro' and 'Sansa' cultivars. In particular, 'Hongro'/M.7 did not show tree vigor reduction due to heavy crop load, and the degree of biennial bearing in 'Sansa'/M.7 was not particularly high. These results indicated that high-density apple planting using the M.7 rootstock was achievable using the 'Hongro' and 'Sansa' cultivars.

• Journal of Bio-Environment Control
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• v.15 no.4
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• pp.352-357
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• 2006

Results of development and activity of roots by rootstock sort of oriental melon were followed; Grafted seedlings had higher root growth than self-roots seedlings and among grafted seedlings, Shintozoa and Elite rootstocks had higher root growth. Grafted seedlings had more xylem exudate, which connects activity of roots, than self-root seedlings. Development of roots and increase of xylem exudate were better in higher temperature. Marketable yield was higher in grafted seedling and quality of fruit was higher in self-root seedling.

• The Plant Pathology Journal
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• v.19 no.5
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• pp.235-238
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• 2003

The resistance to crown gall in grape rootstocks was evaluated by inoculating cuttings from 27 grape rootstock varieties with Agrobacterium vitis strain Cheonan 493. Thmors were formed in all varieties of grape rootstocks tested in this study. The symptoms were observed in the stems of all plants tested including '196-17' and '41B'. Based on the measurement of tumor weight on the stems of grape rootstocks, '779P' was found to be higbly susceptible to crown gall. While some varieties such as 'Gloire', '140R', '101-14M', '3309C', and '333EM' were found to be relatively resistance to crown gall. Varieties such as '99R', '1447P', 'Rupestris du lot', 'll0R', 'Freedom', and '41B' were also found to be susceptibl~ to crown gall. Other~ varieties including 'l103P', 'Teleki. 5C', '420A', 'Golia', and '5BB' were found to be moderately susceptible.

• Journal of Bio-Environment Control
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• v.18 no.4
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• pp.377-384
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• 2009

This study was conducted to examine the effects of electrical conductivity (EC) and rootstock on initial growth and physiological response of grafted pepper in protected cultivation. The pepper (Capcicum annuum L.) cultivars 'Nokgwang' was used as scions, and the cultivars used as rootstocks were Capcicum annuum L: 'Kataguruma', 'Conesian hot' and 'Tantan'. The scion cultivar left ungrafted was used as a control. Two experiments were to examine the effects of the EC levels of nutrient solution on the growth and physiological response of grafted pepper, respectively. Nutrient solution was supplied with three level (1.5, 3.0, 5.0dS/m). By the change of nutrient solution EC level, the plant growth of all seedlings decreased with the increase in EC level. grafted seedling was grafted onto rootstock cultivar 'kataguruma' showed higher growth than the other cultivar at the EC 5.0dS/m level. But this result was slightly different by cultivation time (spring and fall). The total N and P concentration were increased with the increase in EC level, but the Ca and Mg concentration were decreased. Photosynthetic rate of ungrafted seedlings decreased at the EC 5.0dS/m level. But there was no difference between EC 1.5 and 3.0dS/m level. Grafted seedlings showed lower photosynthetic rate at the EC 5.0dS/m level. The activity of SOD do not have a uniformly tendency by the EC level. With the EC 5.0dS/m level, the activity of APX attained higher level than the other EC level. Further study will be needed to examine additional cultivation experiment for more variable rootstock, and development of rootstock for salinity tolerance.

• Journal of Bio-Environment Control
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• v.20 no.3
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• pp.204-210
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• 2011

This experiment was conducted to investigate the effect of water states (water, osmotic potential and turgor pressure) of fruit vesicle and leaf on soluble solids and organic acid contents of fruits of 'Shiranuhi' mandarin hybrid, M16 A line during the fruit maturing season in plastic house cultivation. The 'Shirauhi' grafted on 'Swingle citrumelo', strong strength of rootstock, produced fruit with lower soluble solids and organic acid content than 'Trifoliate' orange rootstock. The fruits vesicle water potential and turgor pressure measured before dawn in 'Swingle citrumelo' were higher tendency than the 'Trifoliate' orange, but osmotic potential values were lower than the 'Trifoliate' orange. The changes of leaf water potential were very similar to the fruit. The results suggest that in the 'Shirauhi' fruits grafted on two rootstocks changes of soluble solids and organic acid content of the fruit were influenced by the leaf water potential and the osmotic potential of the fruit vesicles, which might be caused by the difference of root distribution between two rootstocks.

• Research in Plant Disease
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• v.23 no.3
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• pp.228-233
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• 2017

Six commercial rootstock varieties were tested for resistance to bacterial wilt (Ralstonia solanacearum) by dipping the roots of seedlings in the bacterial suspension at seedling stage. 'Shincheonggang' and 'B-Blocking' ranked top and second in resistance to bacterial wilt, respectively. For disease index of the grafted plants, 'Dotaerang' grafted onto 'Shincheonggang' was the least, followed by 'Ultra', 'Fighting', 'B-Blocking', 'Special', and 'Connection' grafts. Thus, disease development on the grafted plants was correlated with resistance of rootstocks. The effect of rootstocks on horticultural characteristics including days to flower of scion was minimum or negligible. In correlation between horticultural characteristics of rootstocks and scions grafted thereon, statistically significant negative correlation was observed in days to flower and fruit height but no significant correlation was observed in yield per plant, fruit diameter and soluble solid content in the fruits. Thus, selection of resistant rootstock varieties that support the growth and yield of scion well is necessary for effective control of the disease and yield increase.

• Journal of Plant Biotechnology
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• v.42 no.1
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• pp.49-54
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• 2015

Based on the results in this study, here we propose a systematic micropropagation process for 'Gisela 5' that is one of the important dwarfing cherry rootstocks. When the apical tips detached from newly developed shoot in spring season were cultured on the half strength MS media with 0.5 mg/L IBA and 0.5 ~ 1.0 mg/L BA, the cultures scored the highest acquisition rate at 90% for normal shoot with vigorous growth and without hyperhydricity. As next step, the young shoots maintained in vitro well multiplied on the full strength MS medium supplemented with 0.5 mg/L IBA and 0.5 mg/L BA, in which multiplication rate was approximately nine-fold. Given the half strength MS medium containing 2.0 mg/L IBA, each transplanted shoot further developed robust roots. Finally, the plantlets were easily acclimatized in the compost consisted of vermiculite, perlite, and peatmoss in the proportion of 1:1:1. We expect that the results are useful for cherry cultivation and its rootstock production.

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.1
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• pp.5-13
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• 2019

The first commercial planting of sweet cherry in Korea is thought to be done in early 1920s. The planting area of sweet cherry in Korea is about 500 ha in 2017. Although sweet cherry is considered a minor fruit in Korea, it is one of premium fruits in market and popular among children and women, especially among pregnant women. The import of sweet cherry fruits has increased dramatically in recent years. Seedlings of Prunus lannesiana or clones of 'Colt' (P. avium × P. pseudocerasus) were usually used as sweet cherry rootstocks in Korea. During recent decades new dwarfing rootstocks for cherry such as 'Gisela' series were developed in Germany. Among them, several 'Gisela' series have been mainly used in international nurseries. In this study, we investigated optimum rooting condition of a dwarfing rootstock 'Gisela 5' in summer season cuttings. Among eight soil conditions tested, saprolite + commercial nursery soil (1:1) and saprolite + vermiculite (1:1) showed higher rooting percentage than others, showing 93.6% and 88.9%, respectively. More than 95% of 'Gisela 5' rootstocks produce roots when it was treated with IBA for several seconds just before cuttings, irrespective of concentrations (500 to 2000 mg·L^-1).

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2000.10b
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• pp.19-22
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• 2000

접목을 실시할 때 접수와 대목의 적정한 생육은 접목을 용이하게 할 뿐만 아니라 활착 후 접목묘의 묘소질 지속에도 커다란 영향을 미칠 수 있다. 자연광 조건에서 육묘를 실시하면 묘의 생육이 불균일하여 묘소질이 일정치 않게 된다. 그러므로 인공광을 이용한 폐쇄된 환경 조건에서 육묘가 바람직하나, 대목용 박에 대한 적정 광합성 유효광량자속 또는 광주기가 제시되지 않고 있다. 본 연구의 목적은 대목용 박의 초기 생육에 미치는 광합성 유효광량자속과 광주기의 영향을 구명하는 데 있다. (중략)