Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Optimization of apical tip culture condition for In Vitro propagation of 'Gisela 5' dwarf cherry rootstock

양앵두 왜성대목 'Gisela 5'의 기내번식을 위한 정단배양조건의 최적화

  • Xu, Junping (Department of Horticultural Science, College of Agriculture & Life Sciences, Kyungpook National University) ;
  • Kang, In-Kyu (Department of Horticultural Science, College of Agriculture & Life Sciences, Kyungpook National University) ;
  • Kim, Chang Kil (Department of Horticultural Science, College of Agriculture & Life Sciences, Kyungpook National University) ;
  • Han, Jeung-Sul (Department of Horticultural Science, College of Agriculture & Life Sciences, Kyungpook National University) ;
  • Choi, Cheol (Department of Horticultural Science, College of Agriculture & Life Sciences, Kyungpook National University)
  • 쉬쥔핑 (경북대학교 농업생명과학대학원 원예과학과) ;
  • 강인규 (경북대학교 농업생명과학대학원 원예과학과) ;
  • 김창길 (경북대학교 농업생명과학대학원 원예과학과) ;
  • 한증술 (경북대학교 농업생명과학대학원 원예과학과) ;
  • 최철 (경북대학교 농업생명과학대학원 원예과학과)
  • Received : 2015.03.05
  • Accepted : 2015.03.14
  • Published : 2015.03.31
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Abstract

Based on the results in this study, here we propose a systematic micropropagation process for 'Gisela 5' that is one of the important dwarfing cherry rootstocks. When the apical tips detached from newly developed shoot in spring season were cultured on the half strength MS media with 0.5 mg/L IBA and 0.5 ~ 1.0 mg/L BA, the cultures scored the highest acquisition rate at 90% for normal shoot with vigorous growth and without hyperhydricity. As next step, the young shoots maintained in vitro well multiplied on the full strength MS medium supplemented with 0.5 mg/L IBA and 0.5 mg/L BA, in which multiplication rate was approximately nine-fold. Given the half strength MS medium containing 2.0 mg/L IBA, each transplanted shoot further developed robust roots. Finally, the plantlets were easily acclimatized in the compost consisted of vermiculite, perlite, and peatmoss in the proportion of 1:1:1. We expect that the results are useful for cherry cultivation and its rootstock production.

본 연구의 결과를 바탕으로 중요한 양앵두 왜성대목의 하나인 'Gisela 5'의 미세번식을 위한 일련의 체계적 과정을 제안한다. 봄철 새롭게 생장한 신초로부터 절취한 정단조직을 IBA 0.5 mg/L와 BA 0.5 또는 1.0 mg/L가 첨가된 1/2MS배지에 치상하였을 때 hyperhydricity가 없고 왕성한 생장을 하는 정상 신초의 획득율이 90%에 달하였다. 다음단계로, 기내에서 유지된 모식물체로부터 채취한 신초를 IBA 0.5 mg/L와 BA 0.5 mg/L가 첨가된 전MS배지에 이식하면 약 9배에 이르는 수준으로 신초가 증식되었다. 증식된 신초는 IBA 2.0 mg/L가 첨가된 1/2MS배지로 이식하면 지상부의 정상생장과 함께 왕성하게 발근하였다. 발근한 유식물체는 비교적 손쉽게 최종적으로 기외 순화시킬 수 있었는데, 기외 이식 상토로는 버미큘라이트, 펄라이트 및 피트모스의 혼합비율을 1:1:1로 하는 것이 가장 적합하였다. 본 연구의 결과가 양앵두 재배와 양앵두 대목의 생산에 유용하게 활용되기를 기대한다.

Keywords

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