Objective: This study aims to investigate the correlation between office and work environment satisfaction levels and workers' musculoskeletal symptoms by conducting surveys asking office workers to state their musculoskeletal symptoms and office and work environment satisfaction levels. Background: The increased number of office workers and their indoor work hours using computers have led to new understandings of the importance of office environment including its temperature, humidity, noise levels, lighting and quality of air, and work environment including monitor arrangement, space arrangement and the functions of desks and chairs. Method: Specific details on office work, office environment satisfaction levels, work environment satisfaction levels, musculoskeletal symptoms, absence from work and treatment due to musculoskeletal related symptoms were analyzed based on the survey answers given by 397 office workers who were the subjects of this research. Results: Office workers showed different characteristics of musculoskeletal symptoms depending on their gender, age, work experience, office hours, break time and time spent on computers. Also, differences in symptoms were found for workers'with different satisfaction levels for office environment (temperature, humidity, noise levels, quality of air, lighting) and work environment (location of monitors, area and display of office space, chairs and desks). ANOVA shows the differences between groups of subjects classified by the experience of work-absences and treatments or pain complaints. Conclusion and Application: Relationship between workers' satisfaction levels with the office and work environment and their musculoskeletal symptoms is expected to be served as essential data for systematic management of the workplace.