Purpose: This study aimed to investigate the effects of rehabilitation hospital nurses' perception of patient safety culture, nursing professionalism, and nursing work environment on patient safety nursing activities. Methods: A cross-sectional design was used with a convenience sample of 230 nurses with more than six months of experience working in rehabilitation hospitals located in D Metropolitan City, South Korea. Data were collected from October 23 to 31, 2023 through a self-administered questionnaire and analyzed by descriptive statistics, independent t-test, one-way ANOVA with post-verification of Scheffé test, Pearson's correlation coefficient, and multiple stepwise regression analysis using SPSS 28.0. Results: The average scores were 3.73±0.35 for perception of patient safety culture, 3.54±0.47 for nursing professionalism, 2.67±0.49 for nursing work environment, and 4.68±0.45 for patient safety nursing activities. Patient safety nursing activities was significantly positively correlated with perception of patient safety culture, nursing professionalism, and nursing work environment. Experience of reporting incidents (β=.19, p=.002), communication and procedure (β=.18, p=.003), frequency of reporting (β=.18, p=.002), total clinical experience (β=.17, p=.004), patient safety (β=.17, p=.005), and direct supervisor/manager (β=.17, p=.008) affected patient safety nursing activities in rehabilitation hospitals. Conclusion: Perception of patient safety culture may increase rehabilitation hospital nurses' patient safety nursing activities.