• Korean Journal of Plant Tissue Culture
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• v.28 no.4
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• pp.205-208
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• 2001

This study was conducted to improve the regeneration efficiency from seed-derived calli of rice by optimizing the copper concentrations in the media. Mature seeds were cultured on MS medium supplemented with copper sulphate (0 to 5.0 mg/L) and 2 mg/L 2,4-D. Callus growth was influenced by the levels of copper sulphate containing with medium, The addition of copper sulphate (2.5 mg/L) in regeneration medium enhanced dramatically the ability of plant regeneration from seed-derived calli. The mean frequency of plant regeneration of 6 indica rices was 27.4% on medium containing copper sulphate, whereas that of the cultivars on copper-free medium was 2.4%. These results suggest that copper sulphate may have an important role in improving regeneration ability of indica rices.

• Korean Journal of Breeding Science
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• v.42 no.1
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• pp.23-27
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• 2010

The objective of this study was to map the gene for reduced embryo size in rice using DNA markers. The reduced embryo size mutant was induced from N-methyl-N-nitrosourea (MNU) treated Taichung 65. Genetic analysis revealed that the phenotype of the reduced embryo was controlled by a single recessive gene, designated as re-1. For mapping the gene controlling embryo size, an $F_2$ population was developed from a cross between the Korean Tongil-type, Milyang 23 (Oryza sativa ssp. indica) and the mutant. The ratio of $F_2$ seeds nearly fitted to 3:1 ratio, indicating that this phenotype was controlled by a single recessive gene. Bulked sergeant analysis was performed with SSR markers. The gene for the reduced embryo size was detected on chromosome 1. The gene was further mapped between two SSR markers, RM315 and RM265 on chromosome 1 (approximately 1.5 Mb interval). The linked markers will facilitate selection of this grain character in a breeding program and provide the foundation for positional cloning of this gene.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.2
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• pp.114-117
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• 1984

To investigate a possibility of screening for the drought resistance of rice varieties in the slope-ridges, this experiment was conducted in Youngnam Crop Experiment Station in 1982. Seeds were vertically seeded on the slope-ridges which has 30 degree slopes from water level at the front to 1 meter height at the back side. A significant positive correlation was observed between the height of slope-ridges and the PF values (Y=2.25 +0.007 X, r =0.990${\ast}\;{\ast}$).The heading date delayed, the culm length was shortened and the yield was decreased in accordance with the height of slope-ridges. Based upon the above results, it was possible to read the drought resistance of the rice varieties tested. Samnambyeo, Milyang 26, Jinjubyeo and Nongrimna 1 were classified as the resistant ones, while Knob 361-1-8-6-149 was susceptible one.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.6
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• pp.545-553
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• 1993

The metalaxyl seed-soaking treatment markedly increased the root-mat formation of the young rice seedling(8- to l0-day-old) for machine transplanting. This experiment was conducted to find out a possible rooting mechanism of the young rice seedling in relation to the exogenous metalaxyl seed-soaking treatment. The rice seeds of Hwaseongbyeo variety were soaked in the solution of metalaxyl for 24 hours at room temperature. Metalaxyl(25% wettable powder), a fungicide, was used at the concentration of 200ppm as a promoting substance on the root-mat formation of the young rice seedling. Seeding rate of the young rice seedling was 220g per seed tray(30${\times}$60${\times}$depth 3cm). Metalaxyl seed treatment was highly effective in root initiation and elongation of the young rice seedling in terms of root activity, the number and length of root, and amino acid content of roots. The exogenous metalaxyl application would also increase the chlorophyll content and photosynthetic rate in the young rice seedling. Those factors resulted in vigorous root-mat formation of the young rice seedling. In particular, alanine is a lead compound of metalaxyl which biochemically plays an important role in energy intermediate of the rice plant. Alanine conjugation with a glucose (N-${\beta}$-glucoside) is probably the mechanism in enhancement of initiation and elongation of rice roots of young rice seedling.

• Korean Journal of Plant Resources
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• v.9 no.3
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• pp.239-242
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• 1996

This experiment was conducted to utilize the basic information for salinity tolerance using Dongjinbyeo, Mangeumbyeo, Hwangkeumcheong, Nihonbare and Norin 29 as Japonica rices. Salinity level, 0.3, 0.6, 0.9, 1.2 and 1.5%, were treated and germination rate, recovering rate and radicle length of seeds were measured. The germination rate showed the range from 92 to 100% at the concentration of 0.6% of NaCl. However, it decreased at 0.9% and rapidly reduced at 1.2% above for all varieties. Recovering rate for salt damage of seeds was favored in Dongjinbyeo, Mangeumtyeo, Hwangkeumcheong about 92 to 98% after two days of transfering, but 73 to 76% in Nihonbare and Norin 29 were represented. The radicle length revealed shorter with decreased salinity level, particularly all varieties showed one centimeter less at the concentration of 1.2% above.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.2
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• pp.128-133
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• 1993

This experiment was carried out to evaluate the germination characteristics of Korean red rice seeds. Ten lines of the long grain and ten lines of the short grain Korean red rices were tested for dormancy, shoot emergence rate at different water and soil depth. Both the long and short grain type seeds of Korean red rice germinated over than 99% immediately after harvest, so that no dormancy was observed in the Korean red rices. Shoot emergence rate of the long grain red rices at 7cm of water depth and that of the short grain red rices at 11cm were 83.3% and 83.5% respectively, while those of the indica check Samgangbyeo and japonica check Seomjinbyeo were 0%. Shoot emergence rates of the long and short grain red rices at 7cm of soil depth were 71.1% and 73.9% respectively, while those of the check varieties Samgangbyeo and Seomjinbyeo were 40.0% and 28.0% respectively. Thus, the Korean red rices were expected to be useful germplasm source for direct seeding.

• Korean Journal of Environmental Agriculture
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• v.34 no.4
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• pp.260-267
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• 2015

BACKGROUND: The current study was conducted to examine the species specific accumulation of Cd and Pb in 11 crop species (Soybean, Sesame, Corn, Polished rice, Carrot, Potato, Garlic, Spring onion, Chinese leek, Red pepper, Eggplant), through cultivating them under the same condition with metal contaminated soils.METHODS AND RESULTS: Eleven crop species were cultivated in three different soils contaminated with Cd and Pb and harvested. Edible parts of each crop was pretreated and analyzed to determine Cd and Pb concentrations, and subsequently bioconcentration factors (BCFs) were calculated. In general, the crops of which seeds are used as food showed high concentrations of both Cd and Pb. For instance, Cd concentrations in crops cultivated in Soil A was in the order of soybean (0.432 mg kg^-1) > sesame (0.385) > polished rice (0.176) > carrot (0.116) > corn (0.060) > red pepper > (0.047) > potato (0.044) > egg plant (0.025) > garlic (0.023) > spring onion (0.016) > Chinese leek (0.011). BCFs showed the same order.CONCLUSION: From this study, it can be conclude that seeds plants should not be cultivated in Cd and Pb contaminated soils to secure food safety from metal contaminated soils.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.2
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• pp.179-185
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• 1984

These studies were carried out to evaluate the feasibility of using Alkali Digestibility Value (ADV) of soybean seeds as a selection criterion for cooking quality of soybean grain with rice, and to find out an optimal testing condition, varietal differences in ADV and the effect of cultural environment on the ADV, and its mode of inheritance. The ADV test was achieved succesfully by soaking the soybean grain for 24 hours in 25 to 30% KOH solution at 25 to $30^{\circ}C$. Varietal differences in the ADV were conspicuous, but cultural conditions affected the ADV little. All collected lines for cooking with rice showed high ADV and all the small grain cultivars introduced from abroad had low ADV, while recommended cultivars showed great variation in ADV. The characteristic of low ADV in soybean grains was controlled by single dominant gene.

• BMB Reports
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• v.57 no.2
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• pp.79-85
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• 2024

Pre-harvest sprouting is a critical phenomenon involving germination of seeds in the mother plant before harvest under relative humid conditions and reduced dormancy. In this paper, we generated HDR mutant lines with one region SNP (C/T) and an insertion of 6 bp (GGT/GGTGGCGGC) in OsERF1 genes for pre-harvest sprouting (PHS) resistance using CRISPR/Cas9 and a geminiviral replicon system. The incidence of HDR was 2.6% in transformed calli. T₁ seeds were harvested from 12 HDR-induced calli and named ERF1-hdr line. Molecular stability, key agronomic properties, physiological properties, and biochemical properties of target genes in the ERF1-hdr line were investigated for three years. The ERF1-hdr line showed significantly enhanced seed dormancy and pre-harvest sprouting resistance. qRT-PCR analysis suggested that enhanced ABA signaling resulted in a stronger phenotype of PHS resistance. These results indicate that efficient HDR can be achieved through SNP/InDel replacement using a single and modular configuration applicable to different rice targets and other crops. This work demonstrates the potential to replace all genes with elite alleles within one generation and greatly expands our ability to improve agriculturally important traits.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.4
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• pp.235-244
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• 2004

In this study, we compared the levels of methylotrophic bacterial community diversity in the leaf samples of 19 rice cultivars collected from three regions of Korea. Nineteen pink pigmented isolates showing characteristic growth on methanol were obtained. Physiological and biochemical characters of each isolate were examined according to methods described in Bergey's Manual of Systematic Bacteriology. When phylotypes were defined by performing numerical analysis of 37 characteristics, four distinct clusters were formed. The two reference strains, Methylobacterium extorquens AM1 and Methylobacterium fujisawaense KACC10744 were found to group under cluster IV and cluster III respectively. Cluster I diverged on the basis of nitrate reduction and four isolates showed tolerance upto 0.5 M NaCl concentrations. Two strains in cluster I and III were found to possess methane utilizing properties. Most of the isolates in all the four clusters utilized monosaccharides, disaccharide and polyols as carbon source. When the isolates were subjected for indole-3-acetic acid (IAA) analysis in the presence of L-tryptophan, only 8 isolates exhibited IAA production. In addition, the nitrogen source in the medium was found to influence the IAA production. Addition of $(NH_4)_2SO_4$ in the medium led to a 2 to 30 fold increase in the indole synthesis. However, $KNO_3$, $NH_4NO_3$ and $NH_4Cl$ substitution did not significantly stimulate the synthesis of IAA in the growth medium. Result of gnotobiotic root elongation assay significantly increased roots and shoots lengths, and number of lateral roots, which is mediated by IAA production in the culture medium. The rice seedlings primary roots from seeds treated with methylotrophic isolates were on average 27 to 56% longer than the roots from seeds treated with the uninoculated seeds. In addition, application of different high concentrations of authentic IAA ($400g\;mL^{-1}$) to roots of rice seedlings inhibited root growth. However, the IAA concentration from 10 to $200g\;mL^{-1}$, IAA promoted root growth of rice seedlings. These results suggest that bacterial IAA plays a major role in the development of the host plant root system.