Effect of Copper on Callus Formation and Plant Regeneration in Seed Culture of Rice

벼 종자배양에서 Copper가 캘러스 형성 및 식물체 재분화에 미치는 영향

  • 권용삼 (경북대학교 농과대학 농학과) ;
  • 손재근 (경북대학교 농과대학 농학과)
  • Published : 2001.07.01


This study was conducted to improve the regeneration efficiency from seed-derived calli of rice by optimizing the copper concentrations in the media. Mature seeds were cultured on MS medium supplemented with copper sulphate (0 to 5.0 mg/L) and 2 mg/L 2,4-D. Callus growth was influenced by the levels of copper sulphate containing with medium, The addition of copper sulphate (2.5 mg/L) in regeneration medium enhanced dramatically the ability of plant regeneration from seed-derived calli. The mean frequency of plant regeneration of 6 indica rices was 27.4% on medium containing copper sulphate, whereas that of the cultivars on copper-free medium was 2.4%. These results suggest that copper sulphate may have an important role in improving regeneration ability of indica rices.


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