Analysis and mapping of the re-1 gene for reduced embryo size in rice

  • Kien, Trinh Hong (Department of Agronomy, College of Agriculture and Life Science, Chungnam National University) ;
  • Oh, Ji Min (Department of Agronomy, College of Agriculture and Life Science, Chungnam National University) ;
  • Yang, Paul (Department of Agronomy, College of Agriculture and Life Science, Chungnam National University) ;
  • Hong, Soon Kwan (College of Biomedical Sciences, Kangwon National University) ;
  • Ahn, Sang Nag (Department of Agronomy, College of Agriculture and Life Science, Chungnam National University)
  • Received : 2010.03.15
  • Published : 20100300

Abstract

The objective of this study was to map the gene for reduced embryo size in rice using DNA markers. The reduced embryo size mutant was induced from N-methyl-N-nitrosourea (MNU) treated Taichung 65. Genetic analysis revealed that the phenotype of the reduced embryo was controlled by a single recessive gene, designated as re-1. For mapping the gene controlling embryo size, an $F_2$ population was developed from a cross between the Korean Tongil-type, Milyang 23 (Oryza sativa ssp. indica) and the mutant. The ratio of $F_2$ seeds nearly fitted to 3:1 ratio, indicating that this phenotype was controlled by a single recessive gene. Bulked sergeant analysis was performed with SSR markers. The gene for the reduced embryo size was detected on chromosome 1. The gene was further mapped between two SSR markers, RM315 and RM265 on chromosome 1 (approximately 1.5 Mb interval). The linked markers will facilitate selection of this grain character in a breeding program and provide the foundation for positional cloning of this gene.

Keywords

Acknowledgement

Grant : Human Resource Development Center for Economic Region Leading Industry

Supported by : National Research Foundation of Korea (NRF)

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