Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Analysis and mapping of the re-1 gene for reduced embryo size in rice

  • Kien, Trinh Hong (Department of Agronomy, College of Agriculture and Life Science, Chungnam National University) ;
  • Oh, Ji Min (Department of Agronomy, College of Agriculture and Life Science, Chungnam National University) ;
  • Yang, Paul (Department of Agronomy, College of Agriculture and Life Science, Chungnam National University) ;
  • Hong, Soon Kwan (College of Biomedical Sciences, Kangwon National University) ;
  • Ahn, Sang Nag (Department of Agronomy, College of Agriculture and Life Science, Chungnam National University)
  • Received : 2010.03.15
  • Published : 20100300
⟨ Previous Next ⟩

Abstract

The objective of this study was to map the gene for reduced embryo size in rice using DNA markers. The reduced embryo size mutant was induced from N-methyl-N-nitrosourea (MNU) treated Taichung 65. Genetic analysis revealed that the phenotype of the reduced embryo was controlled by a single recessive gene, designated as re-1. For mapping the gene controlling embryo size, an $F_2$ population was developed from a cross between the Korean Tongil-type, Milyang 23 (Oryza sativa ssp. indica) and the mutant. The ratio of $F_2$ seeds nearly fitted to 3:1 ratio, indicating that this phenotype was controlled by a single recessive gene. Bulked sergeant analysis was performed with SSR markers. The gene for the reduced embryo size was detected on chromosome 1. The gene was further mapped between two SSR markers, RM315 and RM265 on chromosome 1 (approximately 1.5 Mb interval). The linked markers will facilitate selection of this grain character in a breeding program and provide the foundation for positional cloning of this gene.

Keywords

Acknowledgement

Grant : Human Resource Development Center for Economic Region Leading Industry

Supported by : National Research Foundation of Korea (NRF)

References

  1. Causse MA, Fulton TM, Cho YG, Aim SN, Chunwongse J, Wu K, Xiao J, Yu Z, Ronald PC, Harrington SE, Second G, McCouch SR, Tanksley SD. 1994. Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138:1252-1274.
  2. Dong Y, Tsuzuki E, Kamiunten H, Terao H, Lin D. 2003. Mapping of QTL for Embryo size in rice. Crop Sci. 43:1068-1071. https://doi.org/10.2135/cropsci2003.1068
  3. Hong SK, Aoki T, Kitano H, Satoh H, Nagato Y. 1995. Temperature-sensitive mutation, embryoless 1, affects both embryo and endosperm development in rice. Plant Sci. 108:165-172. https://doi.org/10.1016/0168-9452(95)04128-H
  4. Hong SK, Kitano H, Satoh H, Nagato Y. 1996. How is embryo size genetically regulated in rice. Development 122:2051-2058.
  5. Juliano BO. 1985. Rice chemistry and technology. American Association of cereal chemists. pp. 17-174.
  6. Kien TK. 2010. Mapping of the re-1 gene for reduced embryo size in rice. M.S. Dissertation of Chungnam National University.
  7. Koh HJ, Park SZ, Won YJ, Heu MH. 1994. Nutritional and agronomic characteristics of super-grain embryo mutant in rice. Korean J. Crop Sci. 38:537-554.
  8. Kon HJ, Heu MH, McCoucn SR. 1996. Molecular mapping of the $ge^s$ gene controlling the super-giant embryo character in rice (Oryza sativa L.). Thear. Appl. Genet. 93: 257-261. https://doi.org/10.1007/BF00225754
  9. Koh HJ, Myung J, Heu MH, McCouch SR. 1998. Tagging of the $ge^s$ gene confering the super-giant embryo character using RFLP, RAPD, and microsatellite markers in rice. Korean J. Breed, 28(4):436-444.
  10. Kosambi DD. 1944. The estimation of map distance from recombination values. Ann. Eugen. 12:172-175.
  11. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L. 1987. MAPMAKER: an interactive computer pack-age for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174-181. https://doi.org/10.1016/0888-7543(87)90010-3
  12. McCoueh SR, Teytelman L, Xu Y, Lobos KG, Clare K, Walton M, Fu B, Maghirang R, Li Z, Xing Y, Zhang Q, Kono I, Yano M, Fjellstrom R. DeClerck G, Schneider D, Cartinhour S, Ware D and Stein D. 2002. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res. 9:199-207. https://doi.org/10.1093/dnares/9.6.199
  13. Nagasawa N, Nagato Y, Saikai H. 2003. Physical mapping of the reduced embryo 2 gene. Rice Genet. Newsl. 20:22-24.
  14. Pallaud O, Chen X, McCouch SR. 1996. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol. Gen. Genet. 252:597-607.
  15. Satoh H, Omura T. 1981. New endosperm mutations induced by chemical mutagens in rice Oryza sativa L. Japan. J. Breed. 31(3):316-326.
  16. Satolt H, Iwata N. 1990. Linkage analysis in rice. On three mutant loci for emdosperm properties, ge (grain embryo). du-4 (dull endosperm-4) and flo-1 (floury endosperm-1). Japan. J. Breed. 40 (Suppl. 2):268-269.