• Journal of Life Science
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• v.26 no.2
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• pp.164-173
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• 2016

Isotocin (IT), a nonapeptide homolog of oxytocin in mammals, has been suggested to be involved in physiological processes including social behaviors, stress responses, and osmoregulation in teleost fish. To study its structure and function, the gene encoding the IT precursor was cloned from the genomic DNA and brain cDNA of the cinnamon clownfish, Amphiprion melanopus. The IT precursor gene consists of three exons separated by two introns, and encodes an open reading frame of 156 amino acid (aa) residues, comprising a putative signal peptide of 19 aa, a mature IT protein of 9 aa, a proteolytic processing site of 3 aa, and 125 aa of neurophysin. Tissue-specific analysis of the IT precursor transcript indicated its expression in the brain and gonads of A. melanopus. To examine its osmoregulatory effects, the salinity of the seawater (34 ppt) used for rearing A. melanopus was lowered to 15 ppt. Histological analysis of the gills indicated the apparent disappearance of an apical crypt on the surface of the gill lamella of A. melanopus, as pavement cells covered the surface upon acclimation to the lower salinity. The level of Na⁺/K^+-ATPase activity in the gills was increased during the initial stage of acclimation, followed by a decrease to its normal level, suggesting its involvement in osmoregulation and homeostasis. The only slight increase in the level of IT precursor transcript in the A. melanopus brain upon low-salinity acclimation suggested that IT played a minor role, if any, in the process of osmoregulation.

• Journal of Life Science
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• v.21 no.2
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• pp.194-201
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• 2011

Caudal-related homeodomain proteins play critical roles in intestine development and maintenance from Drosophila to humans. The loss or reduction of CDX1 and CDX2 are known to be associated with colon cancers. It has been well known that colorectal carcinogenesis is associated with serious oxidative stress and that catalase is decreased in colon carcinomas. However, the underlying molecular mechanisms remain elusive. Here, we report that Caudal-related homeodomain proteins positively regulate catalase expression in both Drosophila and humans. We found that Drosophila caudal heterozygotes have a decreased catalase expression and increased ROS generation in the hindgut, and that the overexpression of Caudal increases catalase promoter activity and catalase mRNA levels. We also found that CDX1 and CDX2 up-regulate catalase promoter activity and protein levels in HCT116 cells - human colorectal carcinoma cell lines. The level of catalase protein in several colorectal carcinoma cell lines was associated with CDX1 expression. These results suggest that CDX1 and CDX2 may be involved in intestinal homeostasis and tumorigenesis via regulation of catalase expression.

• Journal of Life Science
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• v.23 no.2
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• pp.324-331
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• 2013

The addition and removal of N-acetylglucosamine (GlcNAc) molecules on serine or threonine residues of a protein is called O-GlcNAcylation. This post-translational modification occurs on both cytoplasmic and nuclear protein, and is fast and reversible as comparable to phosphorylation. In contrast to the phospho-signaling cycles, this emerging moon-lightening signaling is cycled by only two enzymes, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). The simple machinery is a good evolutionary adaptation of a cell for quick accommodation to continuously fluctuating intra- and extracellular microenvironments. Rather than "switching" on or off a specific proteins - this would be done by phosphorylation where numerous specific kinases and phosphatases are involved - O-GlcNAcylation would play a "rheostat" which would be much more delicately increase or decrease the efficacy of signal transductions in response to cellular nutrient and stress conditions. Interestingly, recent evidence indicates that O-GlcNAc is further modified by phosphorylation. The O-GlcNAc-P will upgrade the modulation efficiency of cellular processes to continuous 'analogue' level. So far, only one protein AP180 was reported to have O-GlcNAc-P on Thr310. But, proteomic data from our laboratory indicate that there are multiple O-GlcNAc-P proteins, constituting "O-GlcNAc-P'om". This will focus on the possibility of existence of "O-GlcNAc-P'om".

• Journal of Life Science
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• v.12 no.3
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• pp.274-280
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• 2002

The metabolites related to phenylpropanoid pathway play an important role in the self-defense of plants and induced by environmental stress like wounding, pathogen attack, UV-irradiation and so on. The mRNA level of rite phenylalanine ammonia lyase (PAL) was increased at 12 h to 48 h, however it was gradually decreased 48 h to 60 h after UV irradiation. The PAL enzyme activities in rice were peaked at the time of 24 h after UV irradiation, on the other hand, it was not affected by wounding. The PAL enzyme activities in pepper were raised high at 24 h and 10 h by UV irradiation and wounding respectively. The cinnamic acid 4-hydroxylase (C4H) activities were increased by wounding treatment and were detected from 12 h to end time point of experiment, while UV-irradiation didn't affect the C4H activity in rice and pepper. These results were assumed that the action of isoflavonid has an alternative effect on the defenses which include wounding and UV irradiation and on the diverse roles in rice and hot pepper.

• Journal of dental hygiene science
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• v.9 no.2
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• pp.181-187
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• 2009

Oral diseases may hinder people from living a healthy life by causing obstacles in the nutrition supply of the human body. This study aims at the found out the eating habits and recognition factors of people who are currently suffering from denial pain, and made a predictive modeling using neural network, which is a data mining. The oral health condition for maintaining and improving oral health has been examined and analyzed through a survey and the groups were divided based on the presence and the absence of dental pain. This study observed on eating habits, exercise and oral habits. The study results of neural network modeling input parameter was selected significant survival factors. As a result of making a predictive modeling using the neural network, the fitness of the predictive modeling of dental pain factors was 88.7%. As for the people who are likely to experience dental pain predicted by the neural network model, preventive measures including proper eating habits, education on oral hygiene, and stress release must precede any dental treatment.

• Journal of agriculture & life science
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• v.45 no.5
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• pp.17-23
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• 2011

Growth characteristics and adaptability of 10 poplar clones planted at a reclaimed tidal flat were evaluated. The contents of $Na^+$, $Ca^{2+}$ and $Mg^{2+}$ were 10.0, 3.4 and 1.5 times higher, respectively than those of control although the electrical conductivity(EC) in the soil at the test plantation was low as much as 0.51 dS/m. The contents of organic matter(OM) and total nitrogen(TN) in the soil were 22.9 and 23.0 times lower than those of control. Average survival rate of 10 poplar clones showed 88% at three years after planting. Clones Eco28(Populus euramericana), Dorskamp(Populus deltoides ${\times}$ P. nigra) and I-476(Populus euramericana) showed the best survival rate of 100%. However, clones 97-19(Populus deltoides(Lux) ${\times}$ P. deltoides(Harvard)) and Suwon (Populus koreana ${\times}$ P. nigra var. italica) were relatively lower than other clones. Average height and DBH of all clones were 4.8 m and 3.6 cm, respectively. Clone Dorskamp showed the greatest height and DBH, 5.9 m and 5.0 cm, respectively. Clones 97-19 and Dorskamp showed the least defoliation by stress and visible damage by insects and diseases, whereas clones Suwon and I-476 were the most sensitive at the reclaimed tidal flat. Clone Dorskamp showed the best adaptability at the reclaimed tidal flat, but clone Suwon showed the worst based on survival rate, growth, and visible damages.

• Journal of Family Resource Management and Policy Review
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• v.25 no.1
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• pp.35-45
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• 2021

Focusing on increasing of single-person households this study aims to develop a scale to measure the healthiness of lifestyle among single-person households. The concept of healthiness of lifestyle is based on the theories of family strength and family ecology. We draw 50 items that encompass basic needs, individual, familial, and social aspects of single-person life. Using a sample of 317 persons who live alone, this study examined a factor structure of the items and selected 44 items based on the results of factor analysis. Reliability and criterion- and construct validity were also examined. The final scale consists of four domains; basic needs (finance, housing, consumption, and future plan), work·life balance (time management, health, and stress), family relations, and social participation (social network, social interests, and community participation). This scale can be used as an assessment measure of the healthiness of lifestyle of single persons who participate in programs in Healthy and Multicultural Families Support Centers.

• Korean Journal of Psychosomatic Medicine
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• v.29 no.2
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• pp.77-85
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• 2021

Breast cancer is the most prevalent oncological disease among women. Various psychosocial distress is common at the diagnosis, treatment, and posttreatment phase of breast cancer. For the treatment of breast cancer, not only medical treatment but also psychosomatic integrative care will be needed. Patients with breast cancer may lead to increased vulnerability to stress, adjustment disorder, anxiety disorder, and depressive disorder, and these psychiatric diseases and conditions are associated with recurrence or exacerbation of breast cancer. Psychosocial treatment of anxiety and depression could increase the quality of life of patients and decrease the recurrence and progression of breast cancer. In this article, we reviewed 5 clinical breast cancer survivorship guidelines focused on psychosomatic integrative care including psychosocial treatment and alternative treatment for psychosocial distress. Because 5 treatment guidelines were using various definitions of evidence, we confirmed evidence of various psychosocial treatments for patients with breast cancer based on the definition of evidence by the US Preventive Service Task Force (USPSTF) guideline. We also reviewed the effect size of psychosocial treatment for anxiety, depression, mood, and quality of life in patients with breast cancer. This article discusses the barrier to the delivery of psychosomatic integrative care and suggests integrative care planning for breast cancer. Multi-disciplinary teams, patient's needs assessment, information technology support, patient and caregiver engagement, planned periodic monitoring of psychosocial distress by a psychosomatic specialist or consultation-liaison psychiatrist are recommended as key features of a psychosomatic integrated care plan.

• Journal of Life Science
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• v.31 no.7
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• pp.641-653
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• 2021

The purpose of this study was to confirm the antifungal activity, plant growth-promoting activity, and mineral solubilizing activity of 18 types of bacteria isolated purely from rhizosphere soil. The potential of isolates of the genus Bacillus and Pseudomonas as biocontrol agents was confirmed through the antifungal activity of these isolates. This activity has been determined to be due to various hydrolytic enzymes on the cell wall of plant pathogenic fungi and the production of siderophores in isolates. In addition, most of the isolates have been found to have aminocyclopropane-1-carboxylate deaminase production activity, indole-3-acetic acid production activity, and nitrogen fixation activity. These characteristics are believed to have a positive effect on root development, growth, and the productivity of crops via a reduction in the concentration of ethylene under conditions of environmental stress, to which plants are commonly exposed. In addition, on testing for the solubilizing activity of the isolates for phosphoric acid, silicon, calcium carbonate, and zinc, some isolates were found to have mineral solubilizing activities. Inoculation of these isolates during plant growth is expected to assist plant growth by converting nutrients necessary for growth into usable forms that can be absorbed by plants. The 18 isolated strains can be used as biocontrol agents due to their antifungal activity, plant growthpromoting activity, and mineral solubilizing activity.

• Journal of Marine Life Science
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• v.4 no.2
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• pp.91-95
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• 2019

The changes of plasma cortisol concentration to high water temperature on common carp, Cyprinus carpio were studied. 20℃, 24℃ and 28℃ groups showed significantly increased plasma cortisol levels until 6 hours, and decreased plasma cortisol levels after 24 hours. Cortisol levels of 20℃ and 24℃ groups decreased to values close to the cortisol content before the experiment. However, 32℃ group showed significantly increased plasma cortisol levels until 24 hours, and maintained plasma cortisol level until 48 hours. The Cumulative mortality rates of 20℃, 24℃ and 28℃ groups increased until 12 hours, and maintained until 48 hours. Cumulative mortality rate of 32℃ group increased continuously until the end of the experiment. Using the results of this study, it could be used as a method to confirm the risk of mass perish in a specific area through hormone analysis.