• 한국산업융합학회 논문집
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• 제20권4호
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• pp.345-350
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• 2017

Saponin is the most pharmaceutical active ingredients of the ginseng plant, it was called "Ginsenoside" which means the Glycoside of ginseng that composed glycosides and aglycones. The human body will absorb the saponin easily if these substrate was decomposed by active microorganism. Fermentation is the most convenient technique to decompose this active ingredients. The purpose of this research was to study the sugar content, pH and acidity development during the ginseng fermentation process. Fresh Korean ginseng and red ginseng extract was used as the main ingredient. The concentrated of pure ginseng extract was added to increase the saponin extract. Furthermore, the mix microbial powder was added as starter to increase the fermentation efficiency. The ginseng was fermented in fermentation chamber at temperature $37^{\circ}C$ during 70 days. In the end of experiment the sugar content was decreased from 24% to 7.65%, The pH was decreased from 6.5 to 3.4, and the acidity level was incresed from 0% to 1.2%.

• 한국산업융합학회 논문집
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• 제21권1호
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• pp.9-16
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• 2018

Aloe gel layer is well known as raw materials of medicines and cosmetics due to their antioxidant and anti-inflammatory properties. In aloe gel extracting process, the outer part of the leaf was removed. It contains high quality of fiber and many nutrients. However, this part is thrown away and generally used as fertilizer. The purpose of this research was to examine the important nutrient of Aloe saponaria. Moreover, the feasibility of using aloe as a dietary supplement by feeding fermentation treatment of aloe was investigated. To do this, the aloe leaf was divided into several parts including leaf skin, bottom of the leaf, tip of the leaf, middle of the leaf, and leaf flesh. Then the saponin content were analyzed from each part. The extraction method was used to clarify the saponin content. The aloe then fermented to improve it benefit. The fermented Aloe then given as dietary food to group of pig. Finally, the appropriate feed level was determined and the pork meat quality was analyzed. The extraction of saponin shows that the highest concentration of saponin located on the skin of the leaf. The feeding experiment shows that there is no significant difference in pig growth without aloe dietary food and groups with aloe as dietary food. It was conclude that fermented aloe can replace the pigs normal feeder as an alternative feeding solution.

• 한국산업융합학회 논문집
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• 제20권5호
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• pp.351-357
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• 2017

Growing plant in potting media without soil is known as Soilless cultivation. This method is used mostly in greenhouse cultivation to increase horticultural commodities production. Peat moss is commonly utilized as potting media substrate because of its characteristic. However, peat moss price is high because of the quantity of peat moss in nature has been decreased. Recently, most of the research is conducted to find the alternative growing medium to cultivate horticulture plant in potting media. Perlite and rice husk ash were mentioned that had a potent as alternative growing media for seasonal plants to increase agriculture production due to the lack of production area. This study aimed to determine the effect of using different substrate and growth performance of chili. The method used was the soilless cultivation. The chili was planted in the pot with perlite media, rice husk ash media, and peat moss media. The chili was measured after 65 days after planting. The result showed that rice husk ash and perlite were more potentials in chili growth performance than peat moss. Rice husk ash had the significant result of plant height. While, Perlite effect on root length, plant weight, leaf length, and stem diameter. The best alternative for cultivation chili without substrate based on this research was perlite then rice husk ash and peat moss.

• 한국산업융합학회 논문집
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• 제20권1호
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• pp.27-33
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• 2017

An agriculture machines are subjected to different loads conditions. Due to this loads variations there will be certain deformations and stress which affect the performance of the electric vehicle in adverse manner. The purpose of this paper is to analyze the total deformation and stress of the electric farm vehicle middle frame based on the finite element method. The proposed electric farm vehicle has lifting and dumping capability. Therefore, in this research four operational condition such as normal condition, dumping condition, lifting condition, and lifting-dumping condition was analyzed. In this research, the design for whole frame structure is elaborated. According to the mechanical characteristics of the frame, materials are selected and manufacturability requirements are limited. Based on ANSYS 15 software, the finite element model of electric farm vehicle is established to carry out static analysis on full-loaded conditions. The simulation results shows that the proposed design meet the strength requirements and displacement requirements. The maximum deformation 0.53611 mm and maximum stress 30.163 MPa occurred at lifting-dumping condition.

• 한국산업융합학회 논문집
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• 제22권1호
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• pp.55-60
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• 2019

Growing plant in potting media without soil is known as Soilless cultivation. This method is used mostly in greenhouse cultivation to increase horticultural commodities production. Peat moss is commonly utilized as potting media substrate because of its characteristic. However, peat moss price is high because of the quantity of peat moss in nature has been decreased. Recently, most of the research is conducted to find the alternative growing medium to cultivate horticulture plant in potting media. Perlite and rice husk ash were mentioned that had a potent as alternative growing media for seasonal plants to increase agriculture production due to the lack of production area. This research aims to determine the growth of in rice husk ash, perlite and peat moss as growing substrates. The method used was the soilless cultivation. The chinese cabbage was planted in the pot with perlite media, rice husk ash media, and peat moss media. The chinese cabbage was measured after 35 days after planting. The result showed that peatmoss was more potentials in chinese cabbage growth performance than rice husk ash and perlite. Peat moss had the significant result of every research parameters such as plant height, plant weight, number of leaves, plant diameter, root length, and root weight. The best alternative for cultivation chinese cabbage without substrate based on this research was peat moss then rice husk ash and perlite.

• Journal of Applied Biological Chemistry
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• 제52권2호
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• pp.84-87
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• 2009

• 한국미생물·생명공학회지
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• 제43권3호
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• pp.280-285
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• 2015

Cotoneaster horizontalis Decne 에탄올 추출물(CHEE)의 항산화능과 항염증 생리활성을 분석하였다. CHEE의 항산화능을 DPPH radical scavenging activity로 분석한 결과 radical 소거능의 정도가 양성 대조군으로 사용한 ascorbic acid와 유사한 정도의 높은 활성을 보여 매우 강한 항산화능을 보유함을 확인하였다. 또한 RAW 264.7 세포주를 이용하여 H₂O₂ 유도에 의해 생성된 ROS에 대한 소거능을 분석한 결과에서도 강한 소거능을 보였다. 뿐만 아니라 항산화효소 HO-1 및 그 전사 인자인 Nrf2의 단백질 발현이 CHEE의 처리에 의해 증가되었다. 한편 CHEE가 LPS에 의해 유도된 NO 생성에 미치는 영향을 분석한 결과 농도의존적인 NO 생성 저해능을 보였으며 이는 NO 생성 단백질인 iNOS의 발현 저해에서 기인함을 확인하였다. 이러한 결과를 통해 C. horizontalis의 항산화능과 항염증 활성을 세포 수준에서 처음으로 확인하였으며 향후 기능성 소재로서 유용하게 활용될 수 있을 것으로 판단된다.

• 한국미생물·생명공학회지
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• 제44권2호
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• pp.117-123
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• 2016

본 연구에서는 C. pubescens Burkill 에탄올 추출물(CPEE)의 항산화능과 항염증 생리활성을 DPPH 라디칼 소거능, ROS 생성 억제능, NO 소거 활성 등을 통해 분석하였다. 먼저 CPEE의 항산화능을 DPPH 라디칼 소거능으로 분석한 결과 강한 소거능을 보였으며, 좀 더 자세한 항산화능 작용기작을 알아보기 위해 ROS 생성 억제능으로 분석한 결과 농도 의존적으로 강한 ROS 소거능을 보였다. 뿐만 아니라 대표적인 항산화 효소인 HO-1 및 그 전사 인자인 Nrf2의 단백질 발현에 미치는 영향을 분석한 결과 CPEE에 의해 HO-1 및 Nrf2의 발현이 증가됨을 보였다. 한편 CPEE가 LPS에 의해 유도된 NO 생성에 미치는 영향을 분석한 결과 농도의 존적인 NO 생성 저해능을 보였으며 이는 NO 생성 단백질인 iNOS의 발현 저해에서 기인함을 확인하였다. 이러한 결과를 통해 CPEE의 높은 항산화능과 항염증 활성을 확인하였으며 향후 잠재적인 기능성 소재로서 유용하게 활용될 수 있을 것으로 판단된다.

• 한국식품영양학회지
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• 제33권4호
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• pp.363-371
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• 2020

This study was designed to evaluate the improvement in exercise endurance by Hovenia dulcis fruit hot water extract (HDWE) for the potential application as a functional food material. The effect was based on the evaluation of the forced-swimming capacity and changes in biochemical parameters in mice. Six-week-old mice were classified into four groups based on a randomized block design: the negative control group, the HDWE 50 mg/kg body weight group, the HDWE 200 mg/kg body weight group, and the 1-octacosanol 50 mg/kg body weight group (n=8 mice per group). The swimming exercise time in the HDWE-50 group was increased at 545±43 sec compared to the control group at 263±28 sec. The HDWE-200 fatigue indicators, lactate acid and ammonia content, decreased to 4.99±0.33 mg/mL and 0.63±0.05 mg/mL, respectively. In addition, the content of glycogen in the liver and muscle tended to increase in the group administered HDWE but was not significant. Likewise, the LDH and glucose contents were increased compared to the control group. The muscle damage indicators ALT (52.7±2.6 U/L) and AST (42.5±5.2 U/L) tended to decrease compared to the control group (76.4±10.2 U/L).

• 한국약용작물학회지
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• 제19권5호
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• pp.373-379
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• 2011

Paeoniae Radix Rubra is a preparation consisting of desiccated roots of Paeonia lactiflora PALL (belonging to Ranunculaceae). Paeoniae Radix Rubra is used as a medicinal herb in Asian countries to treat many diseases. Ethanol- or water-based extracts of Paeoniae Radix Rubra were prepared and tested on RAW 264.7 cells, a murine macrophage cell line. The expression of some pro-inflammatory proteins, including inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphorylated ERK1/2 was detected by Western blot analyses, while PGE2 expression was quantified by ELISA. Both the water and ethanol extracts of Paeoniae Radix Rubra suppressed LPS-induced nitric oxide (NO) production and exhibited cell toxicity in accordance with increased NO production. Also, both extracts reduced the expression of COX-2 and iNOS, and inhibited phosphorylation of ERK1/2 in LPS-stimulated RAW 264.7 cells. Extracts prepared from Paeoniae Radix Rubra contain anti-inflammatory agents that inhibit the iNOS and MAPK pathways.