Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Suppression Effect of the Inflammatory Response in Macrophages by Paeoniae Radix Rubra Extracts

적작약 추출물의 대식세포에 대한 염증억제 효과

  • 박종필 (세명대학교 한방바이오산업임상지원센터) ;
  • 손정현 (세명대학교 한방바이오산업임상지원센터) ;
  • 김용민 (세명대학교 한방바이오산업임상지원센터) ;
  • 정준희 (세명대학교 한의과대학) ;
  • 임강현 (세명대학교 한의과대학) ;
  • 이은용 (세명대학교 한의과대학) ;
  • 김이화 (세명대학교 한방바이오산업임상지원센터)
  • Received : 2011.09.23
  • Accepted : 2011.10.18
  • Published : 2011.10.30
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Abstract

Paeoniae Radix Rubra is a preparation consisting of desiccated roots of Paeonia lactiflora PALL (belonging to Ranunculaceae). Paeoniae Radix Rubra is used as a medicinal herb in Asian countries to treat many diseases. Ethanol- or water-based extracts of Paeoniae Radix Rubra were prepared and tested on RAW 264.7 cells, a murine macrophage cell line. The expression of some pro-inflammatory proteins, including inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphorylated ERK1/2 was detected by Western blot analyses, while PGE2 expression was quantified by ELISA. Both the water and ethanol extracts of Paeoniae Radix Rubra suppressed LPS-induced nitric oxide (NO) production and exhibited cell toxicity in accordance with increased NO production. Also, both extracts reduced the expression of COX-2 and iNOS, and inhibited phosphorylation of ERK1/2 in LPS-stimulated RAW 264.7 cells. Extracts prepared from Paeoniae Radix Rubra contain anti-inflammatory agents that inhibit the iNOS and MAPK pathways.

Keywords

References

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