Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지
DOI QR코드

DOI QR Code

Inhibitory Effects of Angelicae Dahuricae Radix Extract on COPD induced by Cigarette Smoke Condensate and Lipopolysaccharide in Mice

담배연기 응축물과 Lipopolysaccharide의 투여로 유발된 COPD에 대한 백지 추출물의 억제효과

  • Kwak, Ho-Geun (Department of Industrial Plant Science & Technology, Chungbuk National University) ;
  • Lim, Heung-Bin (Department of Industrial Plant Science & Technology, Chungbuk National University)
  • 곽호근 (충북대학교 특용식물학과) ;
  • 임흥빈 (충북대학교 특용식물학과)
  • Received : 2011.09.06
  • Accepted : 2011.10.20
  • Published : 2011.10.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to investigate the inhibitory effect of Angelicae Dahuricae Radix (ADR) extract on chronic obstructive pulmonary disease (COPD) induced by cigarette smoke condensate (CSC) and lipopolysaccharide (LPS) in mice. COPD was induced by intratracheal instillation of LPS and CSC 5 times for 12 days; this increased airway hyperresoponsiveness (AHR) and inflammatory cells in bronchoalveolar lavage fluid (BALF). ADR extract was administered orally at a dose of 50 and 200 mg/kg. The concentration of imperatorin, a major component of ADR and therefore used as a measure of quality control, was $0.098%{\pm}0.018%$. Treatment of the mice with ADR extract (50 and 200 mg/kg) alleviated AHR and reduced inflammatory cell counts. Treatment with cyclosporin A (CSA; 10 mg/kg) also modulated AHR and reduced inflammatory cells effectively. Compared with CSA treatment, treatment with ADR (50 mg/kg) extract reduced neutrophil and $CD4^+/CD3^+$ cell counts by 22.67% and 44.92%, respectively. In addition, compared with CSA treatment, treatment ADR 200 mg/kg reduced neutrophils, $CD4^+/CD3^+$ cells and $CD8^+/CD3^+$ cells, by 32.10%, 83.17% and 82.11%, respectively. These results indicate that ADR extract may have an inhibitory effect on COPD induced by LPS and CSC in mice.

Keywords

References

  1. Barnes PJ. (2000). Chronic obstructive pulmonary disease. The New England Journal of Medicine. 343:269-281. https://doi.org/10.1056/NEJM200007273430407
  2. Barnes PJ. (2008). Drugs for airway disease. Medicine. 36:181- 190. https://doi.org/10.1016/j.mpmed.2008.01.007
  3. Brusselle GG, Bracke KR, Maes T, D'hulst AI, Moerloose KB, Joos GF and Pauwels RA. (2006). Murine models of COPD. Pulmonary Pharmacology & Therapeutics. 19:155-165. https://doi.org/10.1016/j.pupt.2005.06.001
  4. Caramori G and Adcock I. (2003). Pharmacology of airway inflammation in asthma and COPD. Pulmonary Pharmacology & Therapeutics. 16:247-277. https://doi.org/10.1016/S1094-5539(03)00070-1
  5. Chan KH, Ho SP, Teung SC, So WHL, Cho CH, Koo MWL, Lam WK, Ip MSM, Man RYK and Mak JCW. (2009). Chinese green tea ameliorates lung injury in cigarette smokeexposed rats. Respiratory Medicine. 103:1746-1754. https://doi.org/10.1016/j.rmed.2009.04.027
  6. Chen Y, Fan G, Zhang Q, Wu H and Wu Y. (2007). Fingerprint analysis of the fruits of Cnidium monnieri extract by highperformance liquid chromatography-diode array detectionelectrospray ionization tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. 43:926-936. https://doi.org/10.1016/j.jpba.2006.09.015
  7. Daheshia M. (2005). Pathogenesis of chronic obstructive pulmonary disease (COPD). Clinical and Applied Immunology Reviews. 5:339-351. https://doi.org/10.1016/j.cair.2005.09.003
  8. Hamelmann E, Schwarze J, Takeda K, Oshiba A, Larsen GL, Irvin CG and Gelf EW. (1997). Noninvasive measurement of airway responsiveness in allergic mice using barometric plethysmography. American Journal of Respiratory and Critical Care Medicine. 156:766-775. https://doi.org/10.1164/ajrccm.156.3.9606031
  9. Jin HL, Lee BR, Lim KJ, Debneth T, Shin MH and Lim BO. (2011). Anti-inflammatory effects of Prunus mume mixture in colitis induced by dextran sodium sulfate. Korean Journal of Medicinal Crop Science. 19:16-23. https://doi.org/10.7783/KJMCS.2011.19.1.016
  10. Joo EY and Kang WJ. (2005). Analysis on the components of the Angelica dahurica root. Korean Journal of Food Preservation. 12:476-481.
  11. Ketai W, Huitao L, Xingguo C, Yunkun Z and Zhide H. (2001). Identification and determination of active components in Angelica dahurica Benth and its medicinal preparation by capillary electrophoresis. Talanta. 54:753-761. https://doi.org/10.1016/S0039-9140(01)00327-7
  12. Kim KS, Kim YH, Kang KD, Chung SH, Lee PS and Lee SC. (1998). Essential oil content and composition of aromatic constituents in leaf of Saururus chinensis, Angelica dahurica and Cnidium officinale. Korean Journal of Medicinal Crop Science. 6:299-304.
  13. Kwon YS, Kobayashi A, Kajiyama SI, Kawazu K, Kanzaki H and Kim CM. (1997). Antimicrobial constituents of Angelica dahurica roots. Phytochemistry. 44:887-889. https://doi.org/10.1016/S0031-9422(96)00634-6
  14. Larosa DF and Orange JS. (2008). Lymphocytes. Journal of Allergy and Clinical Immunology. 121:S364-S369. https://doi.org/10.1016/j.jaci.2007.06.016
  15. Lechner D, Stavri M, Oluwatuyi M, Miranda RP and Gibbons S. (2004). The anti-staphylococcal activity of Angelica dahurica (Bai Zhi). Phytochemistry. 65:331-335. https://doi.org/10.1016/j.phytochem.2003.11.010
  16. Lee YS, Jang SM and Kim NW. (2007). Antioxidative activity and physiological function of the Angelica dahurica roots. Journal of The Korean Society of Food Science and Nutrition. 36:20-26. https://doi.org/10.3746/jkfn.2007.36.1.020
  17. MacNee W. (2003). COPD: Causes and pathology. Medicine, 31:71-75.
  18. Martorana PA, Cavarra E, Lucattelli M and Lungarella G. (2006). Models for COPD involving cigarette smoke. Drug Discovery Today: Disease Models. 3:225-230.
  19. Meuer SC, Fitzgerald KA, Hussey RE, Hodgdon JC, Schlossman SF and Reinherz EL. (1983). Clonotypic structures involved in antigen-specific human T cell function. Journal of Experimental Medicine. 157:705-719. https://doi.org/10.1084/jem.157.2.705
  20. Petty TL. (2002). COPD: Clinical phenotypes. Pulmonary Pharmacology & Therapeutics. 15:341-351. https://doi.org/10.1006/pupt.2002.0380
  21. Petty TL. (2006). The history of COPD. Interational Journal of COPD. 1:3-14. https://doi.org/10.2147/copd.2006.1.1.3
  22. Ryu SY, Kim JC, Kim YS, Kim HT, Kim SK, Choi GJ, Kim JS, Lee SW, Heor JH and Cho KY. (2001). Antifungal activities of coumarins isolated from Angelica gigas and Angelica dahurica aganist plant pathogenic fungi. The Korean Journal of Pesticide Science. 5:26-35.
  23. Shen T, Kim DJ and Cho JY. (2009). Immunomodulatory effect of Pueraria lobata on the functional activation of macrophages by lipopolysaccharide treatment. Korean Journal of Medicinal Crop Science. 17:8-14.
  24. Shin KH, Kim ON and Woo WS. (1988). Effects of the constituents of Angelicae Dahuricae Radix on hepatic drug metabolizing enzyme activity. Korean Journal of Pharmacognosy. 19:19-27.
  25. Son DW, Lee PJ, Lee JS, Lee SH, Choi SY, Lee JW and Kim SY. (2007). Neuroprotective effect of scopoletin from Angelica dahurica on oxygen and glucose deprivation-exposed rat organotypic hippocampal slice culture. Food Science and Biotechnology. 16:632-635.
  26. Song DK, Kim JY, Li G, Lee KS, Seo CS, Yan JJ, Jung JS, Kim HJ, Chang HW and Son JK. (2005). Agents protecting against sepsis from the roots of Angelica dahurica. Biological and Pharmaceutical Bulletin. 28:380-382. https://doi.org/10.1248/bpb.28.380
  27. Tavares E, Ojeda ML, Maldonado R and Minano FJ. (2004). Neutralization of macrophage inflammatory protein-2 blocks the fedrile response induced by lipopolysaccaride in rats. Journal of Thermal Biology. 29:413-421. https://doi.org/10.1016/j.jtherbio.2004.08.061
  28. Vernooy JHJ, Dentener MA, van Suylen RJ, Buurman WA and Wouters EFM. (2002). Long-term intratracheal lipopolysaccharide exposure in mice results in chronic lung inflammation and persistent pathology. American Journal of Respiratory Cell and Molecular Biology. 26:152-159. https://doi.org/10.1165/ajrcmb.26.1.4652
  29. Vlahos R, Bozinovski S, gualano RC, Ernst M and Anderson GP. (2006). Modelling COPD in mice. Pulmonary Pharmacology & Therapeutics. 19:12-17. https://doi.org/10.1016/j.pupt.2005.02.006
  30. Yamaguchi H, Matsuo J, Sugimoto S, Utsumi M and Yamamoto Y. (2008). Inhibition of lymphocyte CD3 expression by Chlamydophila pneumoniae. Microbial Pathogenesis. 45: 290-296. https://doi.org/10.1016/j.micpath.2008.06.005
  31. Yoon TS, Cheon MS, Kim SJ, Lee AY, Moon CB, Chun JM, Choo BK and Kim HK. (2010). Ecaluation of solvent extraction on the anti-inflammatory efficacy of Glycyrrhiza uralensis. Korean Journal of Medicinal Crop Science. 18:28-33.
  32. Zamoyska R. (1998). CD4 and CD8: Modulators of T-cell receptor recognition of antigen and of immune responses. Current Opinion in Immunology. 10:82-87. https://doi.org/10.1016/S0952-7915(98)80036-8
  33. Zheng H. Liu Y, Huang T, Fang Z, Li G and He S. (2009). Development and characterization of A ret model of chronic obstructive pulmonary disease (COPD) induced by sidestream cigarette smoke. Toxicology Letters. 189:225-234. https://doi.org/10.1016/j.toxlet.2009.06.850
  34. Zheng X, Zhang X, Sheng X, Yuan Z, Yang W, Wang Q and Zhang L. (2010). Simultaneous characterization and quantitation of 11 coumarins in Radix Angelicae Dahuricae by high performance liquid chromatography with electrospray tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. 51:599-605. https://doi.org/10.1016/j.jpba.2009.09.030

Cited by

  1. Antiasthmatic Effects on Scutellaria baicalensis Georgi Extracts Against Airway Inflammation and Hyperresponsiveness Induced by Diesel Exhaust Particles with Ovalbumin Sensitization vol.20, pp.2, 2012, https://doi.org/10.7783/KJMCS.2012.20.2.129
  2. Ligustrum lucidum Fruits Extract Inhibits Acute Pulmonary Inflammation in Mice vol.21, pp.5, 2013, https://doi.org/10.7783/KJMCS.2013.21.5.323