• 제목/요약/키워드: AUX1

검색결과 36건 처리시간 0.031초

현사시나무에서 Auxin/indole-3-acetic acid 1 (Aux/IAA1) 유전자 분리 및 발현 특성 구명 (Isolation and characterization of Auxin/indole-3-acetic acid 1 (Aux/IAA1) gene from poplar (Populus alba × P. glandulosa))

  • 배은경;최영임;이효신;최지원
    • Journal of Plant Biotechnology
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    • 제46권3호
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    • pp.180-188
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    • 2019
  • 옥신은 식물의 생장과 발달 과정에서 중요한 조절자로서 기능한다. 옥신 신호전달 과정은 3개의 주요 옥신 반응 전사인자인 Auxin/indole-3-acetic acid (Aux/IAA), Gretchen Hagen 3 (GH3), 그리고 small auxin up RNA (SAUR) 유전자에 의해 조절된다. 특히, Aux/IAA는 옥신 신호에 반응하여 빠르게 축적되는 수명이 짧은 핵 단백질이다. 이 실험에서 우리는 현사시 나무(Populus alba ${\times}$ P. glandulosa)로 부터 PagAux/IAA1 유전자를 분리하고 발현 특성을 분석하였다. PagAux/IAA1 cDNA는 4개의 보존된 도메인과 2개의 nuclear localization sequence (NLS)을 포함한 200개의 아미노산을 암호화하고 있다. Southern blot 분석으로 현사시나무 genome에 PagAux/IAA1 유전자가 single copy로 존재하는 것을 확인하였다. PagAux/IAA1 유전자는 잎과 꽃에서 특이적으로 발현되었다. 그리고 PagAux/IAA1 유전자는 현탁배양세포의 생장 과정에서 초기 지수생장기에 발현되었다. PagAux/IAA1 유전자의 발현을 분석한 결과, 건조와 염 스트레스 및 식물호르몬인 ABA 처리에 의해 발현이 감소된 반면 저온 스트레스, 형성층의 세포 분열 과정 그리고 식물호르몬인 GA와 JA 처리에서 발현이 증가하였다. 따라서 PagAux/IAA1 유전자가 현사시나무에서 저온 스트레스 반응뿐 아니라 생장 과정에 관여할 것으로 판단된다.

디스플레이포트 인터페이스의 AUX 채널 설계 (A Design of DisplayPort AUX Channel)

  • 차성복;윤광희;김태호;강진구
    • 전기전자학회논문지
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    • 제14권1호
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    • pp.1-7
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    • 2010
  • 본 논문은 디스플레이포트 v1.1a 표준에 적합한 AUX(Auxiliary) 채널 구현에 대한 논문이다. 디스플레이포트는 영상 및 음성을 전달하기 하기 위해 메인 링크, AUX 채널, 핫 플러그 검출 라인을 사용한다. 등시적 전송 서비스를 제공하기 위해서 소스 디바이스는 메인 링크를 통해 전달될 영상 및 음성 신호를 특정 형태로 변환하여 재구성하고 싱크 디바이스로 전달한다. AUX 채널은 메인 링크를 구성하고 유지하기 위해 링크 서비스를 제공한다. 그리고 디스플레이 장치가 소스 디바이스에서 전송된 데이터를 정상적으로 나타낼 수 있는지 파악하기 위해 디바이스 서비스를 제공한다. 핫 플러그 검출 라인은 두 디바이스간의 연결을 확인하기 위해서 사용한다. 본 논문은 AUX 채널 구현을 목표로 설계하였으며 설계된 시스템은 SoC Master3를 이용하여 검증을 수행하였다. 합성 툴은 Xilinx ISE 9.2i를 사용하여 3315개의 LUTs와 1466개의 Flip Flops을 사용하였고 최대 168.782MHz 동작 속도의 결과를 얻었다.

Auxin Induced Expression of Expansin is Alered in a New Aux1 Allele that Shows Severe Defect in Gravitropic Response

  • Jeong, Hae-Jun;Kwon, Ye-Rim;Oh, Jee-Eun;Kim, Ki-Deok;Lee, Sung-Joon;Hong, Suk-Whan;Lee, Ho-Joung
    • Journal of Applied Biological Chemistry
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    • 제49권4호
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    • pp.148-153
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    • 2006
  • While the underlying molecular mechanism remains to be elucidated, recent studies suggest that polar auxin transport is a key controlling factor in triggering differential growth responses to gravity. Identification of regulatory components in auxin-mediated differential cell expansion would improve our understanding of the gravitropic response. In this study, we identify a mutant designated aux1-like(later changed to aux1), an allele of the aux1 mutant that exhibits a severely disrupted root gravitropic response, but no defects in developmental processes. In Arabidopsis, AUX1 encodes an auxin influx carrier. Since in-depth characterization of the gravitropic response caused by mutations in this gene has been performed previously, we focused on identifying the downstream genes that were differentially expressed compared to wild-type plants. Consistent with the mutant phenotype, the transcription of the auxin-responsive genes IAA17 and GH3 were altered in aux1 plants treated with IAA, 2, 4-D and NAA. In addition, we identified two expansin genes EXP10 and EXPL3 that exhibited different expression in wild-type and mutant plants.

Application of data fusion modeling for the prediction of auxin response elements in Zea mays for food security purposes

  • Nesrine Sghaier;Rayda Ben Ayed;Ahmed Rebai
    • Genomics & Informatics
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    • 제20권4호
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    • pp.45.1-45.7
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    • 2022
  • Food security will be affected by climate change worldwide, particularly in the developing world, where the most important food products originate from plants. Plants are often exposed to environmental stresses that may affect their growth, development, yield, and food quality. Auxin is a hormone that plays a critical role in improving plants' tolerance of environmental conditions. Auxin controls the expression of many stress-responsive genes in plants by interacting with specific cis-regulatory elements called auxin-responsive elements (AuxREs). In this work, we performed an in silico prediction of AuxREs in promoters of five auxin-responsive genes in Zea mays. We applied a data fusion approach based on the combined use of Dempster-Shafer evidence theory and fuzzy sets. Auxin has a direct impact on cell membrane proteins. The short-term auxin response may be represented by the regulation of transmembrane gene expression. The detection of an AuxRE in the promoter of prolyl oligopeptidase (POP) in Z. mays and the 3-fold overexpression of this gene under auxin treatment for 30 min indicated the role of POP in maize auxin response. POP is regulated by auxin to perform stress adaptation. In addition, the detection of two AuxRE TGTCTC motifs in the upstream sequence of the bx1 gene suggests that bx1 can be regulated by auxin. Auxin may also be involved in the regulation of dehydration-responsive element-binding and some members of the protein kinase superfamily.

APOLLO2 YEAR 2010

  • Sanchez, Richard;Zmijarevi, Igor;Coste-Delclaux, M.;Masiello, Emiliano;Santandrea, Simone;Martinolli, Emanuele;Villate, Laurence;Schwartz, Nadine;Guler, Nathalie
    • Nuclear Engineering and Technology
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    • 제42권5호
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    • pp.474-499
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    • 2010
  • This paper presents the mostortant developments implemented in the APOLLO2 spectral code since its last general presentation at the 1999 M&C conference in Madrid. APOLLO2 has been provided with new capabilities in the domain of cross section self-shielding, including mixture effects and transfer matrix self-shielding, new or improved flux solvers (CPM for RZ geometry, heterogeneous cells for short MOC and the linear-surface scheme for long MOC), improved acceleration techniques ($DP_1$), that are also applied to thermal and external iterations, and a number of sophisticated modules and tools to help user calculations. The method of characteristics, which took over the collision probability method as the main flux solver of the code, allows for whole core two-dimensional heterogeneous calculations. A flux reconstruction technique leads to fast albeit accurate solutions used for industrial applications. The APOLLO2 code has been integrated (APOLLO2-A) within the $ARCADIA^{(R)}$ reactor code system of AREVA as cross section generator for PWR and BWR fuel assemblies. APOLLO2 is also extensively used by Electricite de France within its reactor calculation chain. A number of numerical examples are presented to illustrate APOLLO2 accuracy by comparison to Monte Carlo reference calculations. Results of the validation program are compared to the measured values on power plants and critical experiments.

Assessment of INSPYRE-extended fuel performance codes against the SUPERFACT-1 fast reactor irradiation experiment

  • L. Luzzi;T. Barani;B. Boer;A. Del Nevo;M. Lainet;S. Lemehov;A. Magni;V. Marelle;B. Michel;D. Pizzocri;A. Schubert;P. Van Uffelen;M. Bertolus
    • Nuclear Engineering and Technology
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    • 제55권3호
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    • pp.884-894
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    • 2023
  • Design and safety assessment of fuel pins for application in innovative Generation IV fast reactors calls for a dedicated nuclear fuel modelling and for the extension of the fuel performance code capabilities to the envisaged materials and irradiation conditions. In the INSPYRE Project, comprehensive and physics-based models for the thermal-mechanical properties of U-Pu mixed-oxide (MOX) fuels and for fission gas behaviour were developed and implemented in the European fuel performance codes GERMINAL, MACROS and TRANSURANUS. As a follow-up to the assessment of the reference code versions ("pre-INSPYRE", NET 53 (2021) 3367-3378), this work presents the integral validation and benchmark of the code versions extended in INSPYRE ("post-INSPYRE") against two pins from the SUPERFACT-1 fast reactor irradiation experiment. The post-INSPYRE simulation results are compared to the available integral and local data from post-irradiation examinations, and benchmarked on the evolution during irradiation of quantities of engineering interest (e.g., fuel central temperature, fission gas release). The comparison with the pre-INSPYRE results is reported to evaluate the impact of the novel models on the predicted pin performance. The outcome represents a step forward towards the description of fuel behaviour in fast reactor irradiation conditions, and allows the identification of the main remaining gaps.

Assessment of three European fuel performance codes against the SUPERFACT-1 fast reactor irradiation experiment

  • Luzzi, L.;Barani, T.;Boer, B.;Cognini, L.;Nevo, A. Del;Lainet, M.;Lemehov, S.;Magni, A.;Marelle, V.;Michel, B.;Pizzocri, D.;Schubert, A.;Uffelen, P. Van;Bertolus, M.
    • Nuclear Engineering and Technology
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    • 제53권10호
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    • pp.3367-3378
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    • 2021
  • The design phase and safety assessment of Generation IV liquid metal-cooled fast reactors calls for the improvement of fuel pin performance codes, in particular the enhancement of their predictive capabilities towards uranium-plutonium mixed oxide fuels and stainless-steel cladding under irradiation in fast reactor environments. To this end, the current capabilities of fuel performance codes must be critically assessed against experimental data from available irradiation experiments. This work is devoted to the assessment of three European fuel performance codes, namely GERMINAL, MACROS and TRANSURANUS, against the irradiation of two fuel pins selected from the SUPERFACT-1 experimental campaign. The pins are characterized by a low enrichment (~ 2 wt.%) of minor actinides (neptunium and americium) in the fuel, and by plutonium content and cladding material in line with design choices envisaged for liquid metal-cooled Generation IV reactor fuels. The predictions of the codes are compared to several experimental measurements, allowing the identification of the current code capabilities in predicting fuel restructuring, cladding deformation, redistribution of actinides and volatile fission products. The integral assessment against experimental data is complemented by a code-to-code benchmark focused on the evolution of quantities of engineering interest over time. The benchmark analysis points out the differences in the code predictions of fuel central temperature, fuel-cladding gap width, cladding outer radius, pin internal pressure and fission gas release and suggests potential modelling development paths towards an improved description of the fuel pin behaviour in fast reactor irradiation conditions.

산-생장설에 대한 최근 연구 동향 (Recent research progress on acid-growth theory)

  • 이상호
    • Journal of Plant Biotechnology
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    • 제43권4호
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    • pp.405-410
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    • 2016
  • Auxins are essential in plant growth and development. The auxin-stimulated elongation of plant cells has been explained by the "acid-growth theory", which was proposed forty years ago. According to this theory, the auxin activates plasma membrane $H^+-ATPase$ to induce proton extrusion into the apoplast, promoting cell expansion through the activation of cell wall-loosening proteins such as expansins. Even though accepted as the classical theory of auxin-induced cell growth for decades, the major signaling components comprising this model were unknown, until publication of recent reports. The major gap in the acid growth theory is the signaling mechanism by which auxin activates the plasma membrane $H^+-ATPase$. Recent genetic, molecular, and biochemical approaches reveal that several auxin-related molecules, such as TIR1/AFB AUX/IAA coreceptors and SMALL AUXIN UP RNA (SAUR), serve as important components of the acid-growth model, phosphorylating and subsequently activating the plasma membrane $H^+-ATPase$. These researches reestablish the four-decade-old theory by providing us the detailed signaling mechanism of auxininduced cell growth. In this review, we discuss the recent research progress in auxin-induced cell elongation, and a set of possible future works based on the reestablished acid-growth model.

SUBTOURNAMENTS ISOMORPHIC TO W5 OF AN INDECOMPOSABLE TOURNAMENT

  • Belkhechine, Houmem;Boudabbous, Imed;Hzami, Kaouthar
    • 대한수학회지
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    • 제49권6호
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    • pp.1259-1271
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    • 2012
  • We consider a tournament T = (V,A). For each subset X of V is associated the subtournament T(X) = (X,$A{\cap}(X{\times}X)$) of T induced by X. We say that a tournament T' embeds into a tournament T when T' is isomorphic to a subtournament of T. Otherwise, we say that T omits T'. A subset X of V is a clan of T provided that for a, $b{\in}X$ and $x{\in}V{\backslash}X$, $(a,x){\in}A$ if and only if $(b,x){\in}A$. For example, ${\emptyset}$, $\{x\}(x{\in}V)$ and V are clans of T, called trivial clans. A tournament is indecomposable if all its clans are trivial. In 2003, B. J. Latka characterized the class ${\tau}$ of indecomposable tournaments omitting a certain tournament $W_5$ on 5 vertices. In the case of an indecomposable tournament T, we will study the set $W_5$(T) of vertices $x{\in}V$ for which there exists a subset X of V such that $x{\in}X$ and T(X) is isomorphic to $W_5$. We prove the following: for any indecomposable tournament T, if $T{\notin}{\tau}$, then ${\mid}W_5(T){\mid}{\geq}{\mid}V{\mid}$ -2 and ${\mid}W_5(T){\mid}{\geq}{\mid}V{\mid}$ -1 if ${\mid}V{\mid}$ is even. By giving examples, we also verify that this statement is optimal.