• Korean Journal of Clinical Laboratory Science
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• v.56 no.1
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• pp.10-20
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• 2024

RNA-sequencing (RNA-seq) is a technique used for providing global patterns of transcriptomes in samples. However, it can only provide the average gene expression across cells and does not address the heterogeneity within the samples. The advances in single-cell RNA sequencing (scRNA-seq) technology have revolutionized our understanding of heterogeneity and the dynamics of gene expression at the single-cell level. For example, scRNA-seq allows us to identify the cell types in complex tissues, which can provide information regarding the alteration of the cell population by perturbations, such as genetic modification. Since its initial introduction, scRNA-seq has rapidly become popular, leading to the development of a huge number of bioinformatic tools. However, the analysis of the big dataset generated from scRNA-seq requires a general understanding of the preprocessing of the dataset and a variety of analytical techniques. Here, we present an overview of the workflow involved in analyzing the scRNA-seq dataset. First, we describe the preprocessing of the dataset, including quality control, normalization, and dimensionality reduction. Then, we introduce the downstream analysis provided with the most commonly used computational packages. This review aims to provide a workflow guideline for new researchers interested in this field.

• Journal of the Korea Organic Resources Recycling Association
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• v.32 no.1
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• pp.49-59
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• 2024

Biodegradable coating urea plays an important role in reducing the non-pollutants in agroecosystems, maximizing the plant nutrient utilization efficiency and slow-releasing nitrogen. Herein, the objective of this study was to investigate the nitrogen-releasing patterns and greenhouse gas emissions on different biodegradable coating urea. The treatments consisted of the control as an application of chemical fertilizers, NBCF as the non-biodegradable coating urea, NB60, and MDS as biodegradable coating urea. As a result of this study, the maximum accumulated total nitrogen (TN) concentration in the NBCF was higher at 33% than one in the NB60 during the precipitation periods. Its leaching period in the NCBF was prolonged for day 10 compared to the NB60. TN and NO₃-N releasing patterns in the NBCF and NB60 were fitted well on linear types(R²≥0.991), but their control and MDS were fitted well on Sigmoid curves(R²≥0.994) with high releasing concentration in the MDS compared to the control during leaching periods. For the greenhouse gas emissions, CH₄ emissions in the NBCF, NB60, and MDS were increased at 0.38%, 11.36%, and 5.91%, and N₂O emissions were also increased at 50.5%, 32.4%, 58.8% as compared to the control, respectively. Therefore, application of biodegradable polymer coating urea might mitigate the non-point pollutants in agro-ecosystem.

• The Journal of the Convergence on Culture Technology
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• v.10 no.3
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• pp.839-844
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• 2024

This study analytically analyzed the impact of underground structure displacement behavior on track damage due to adjacent excavation work, ground deterioration, and changes in groundwater level. The concrete track that was the subject of the study was analyzed for sleeper floating track(STEDEF) and precast concrete slab track(B2S). Sleeper floating track is a track structure in which the concrete bed and sleepers are voided. precast concrete slab track is a track structure that induces the elastic behavior of the rail by assembling rails and fasteners using slabs. For numerical analysis, each concrete track, from rail to concrete bed, was modeled as three-dimensional elements. In addition, the displacement behavior of the underground structure was set as a variable to analyze the damage effect on the concrete bed. Using numerical analysis, the concrete bed stress due to uplift and subsidence was analyzed, and the level of crack effect was analyzed by comparing it to the tensile strength and shear strength. As a result of the analysis, it was found that the sleeper floating track was more vulnerable than the precast concrete slab track when the same uplift and subsidence occurred. In addition, uplift and subsidence, it was analyzed that the cracks range in the sleeper floating track was large.

• The Journal of Sustainable Design and Educational Environment Research
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• v.23 no.2
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• pp.1-10
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• 2024

This study presents a model for educational planning based on user participatory design for the purposes of curriculum development and educational space innovation. It reviewed previous research and practices related to educational space innovation, educational planning processes, and user participation design and suggested a draft model. This model was applied in the renovation project of an elementary school, and the problems encountered during the application were reflected upon and used to improve the model. The final model comprises four phases. Phase 1 is an initial exploratory phase foruser participation, where educational planners and users form a shared perspective regarding the future of education. Phase 2 is a needs-analysis phase for the transition to future education, which focuses on setting the overall direction of the school-level curriculum. Phase 3 specifies the direction of future school curricula and identifies needs for educational spaces. Finally, Phase 4 focuses on the interaction between educational and space planners. Based on the above discussion, the study discussed the benefits and limitations of this educational planning model for school space innovation.

• Education of Primary School Mathematics
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• v.27 no.3
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• pp.303-320
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• 2024

This study presents the development and performance evaluation of a custom GPT-based chatbot tailored to provide solutions following Polya's problem-solving stages. A beta version of the chatbot was initially deployed to assess its mathematical capabilities, followed by iterative error identification and correction, leading to the final version. The completed chatbot demonstrated an accuracy rate of approximately 89.0%, correctly solving an average of 57.8 out of 65 image-based problems from a 6th-grade elementary mathematics textbook, reflecting a 4 percentage point improvement over the beta version. For a subset of 50 problems, where images were not critical for problem resolution, the chatbot achieved an accuracy rate of approximately 91.0%, solving an average of 45.5 problems correctly. Predominant errors included problem recognition issues, particularly with complex or poorly recognizable images, along with concept confusion and comprehension errors. The custom chatbot exhibited superior mathematical performance compared to the general-purpose ChatGPT. Additionally, its solution process can be adapted to various grade levels, facilitating personalized student instruction. The ease of chatbot creation and customization underscores its potential for diverse applications in mathematics education, such as individualized teacher support and personalized student guidance.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.44 no.4
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• pp.531-544
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• 2024

The purpose of this study is to analyze traffic flow characteristics according to the market penetration rate (MPR) of autonomous vehicles (AV) on road sections where bus rapid transit (BRT) is actually operating. Furthermore, the maximum traffic volume was set through estimation of future traffic demand, and traffic flow characteristics were analyzed through traffic simulation for each scenario considering of a combination of BRT introduction and AV's MPR. To test statistical significance, Kruskal-Willis test and Jonckheere-Terpstra test were used to examine the impact of the market penetration rate of Autonomous vehicles on travel time and delay time etc. At the same time, the existence of the order relationship among travel time data according to the market penetration rate of autonomous vehicle was examined. As a result of the analysis, it was founded that the travel time significantly decreased as the MPR of AV increases in both intermittent flow and continuous flow environments. In particular, in the case of continuous flow, the law of increasing returns was satisfied in the effect of increasing travel speed and reducing travel time as the MPR of AV increases. The results of this study are expected to be used as a basic information for design plans for road reconstruction and space utilization after the commercialization of AV in the future.

• Journal of the Korean Society for information Management
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• v.41 no.3
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• pp.33-61
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• 2024

The emergence of Open Access (OA) and other changes to the academic publishing ecosystem have made the prevalence of predatory journals a significant concern. Academic librarians play a crucial role in the academic environment that protects researchers and takes action against predatory journals. Nonetheless, there isn't many research that looks into how academic librarians view and handle predatory journals. This study aims to give an exploratory analysis of the overall perceptions of predatory journals among university librarians in South Korea. A survey was administered to academic librarians between April 10 and May 1, 2024, and 78 respondents' answers were examined. The findings demonstrate that opinions among South Korean academic librarians on predatory journals are not all uniform. Based on their readiness and willingness to supply services, the respondents could be roughly categorized into four groups, according to cluster analysis. The study affirms that training librarians and improving cooperation between academic libraries and researchers are critical prerequisites for the advancement of services aimed at predatory publications. We anticipate that this study, which is the first of its type to explore how academic librarians in South Korea see predatory journals, will be a valuable starting point for university libraries as they plan and offer services related to predatory journals in the future.

• Journal of Music and Human Behavior
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• v.21 no.2
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• pp.77-105
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• 2024

This study explored whether story-based interactive music activities improve play interactions between children with developmental disabilities (DD) and typically developing (TD) children at an integrated daycare center, and whether these activities influence TD children's attitudes towards their DD peers. The participants included six children with DD, aged 3-5 years, and 21 TD peers. They attended 10 sessions, each 30 minutes, twice a week. Play interactions were observed during free play after each session, and TD children's attitudes towards peers with DD were assessed before and after the intervention. The results showed that children with TD and DD in the integrated group showed significantly reduced disengagement, with notable improvements in joint action for TD children and joint attention for DD children. However, there were no significant changes in peer acceptance among TD children in the integrated group while there was a significant decrease in the non-integrated group. This study highlights that story-based interactive music activities effectively enhance interactions between TD and DD children. However, the limited impact on peer acceptance suggests the need for more long-term and systematic interventions, considering the children's developmental levels. The study also emphasizes the importance of tailored approaches that address the individual needs of both TD and DD children, providing valuable insights into the direction of systematic interventions at a time when the importance of social integration is increasingly recognized.

• Journal of the Korean Association of Geographic Information Studies
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• v.27 no.3
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• pp.1-13
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• 2024

This paper proposes a cost-effective system design and user-friendly approach for the key technological elements necessary to configure an autonomous mobile robot. To implement a high-precision positioning system using an autonomous mobile robot, we established a Linux-based VRS (virtual reference station)-RTK (real-time kinematic) GNSS (global navigation satellite system) system with NTRIP (Network Transport of RTCM via Internet Protocol) client functionality. Notably, we reduced the construction cost of the GNSS positioning system by performing dynamic location analysis of the established system, without utilizing an RTK replay system. Dynamic location analysis involves sampling each point during the trajectory following of the autonomous mobile robot and comparing the location precision with ground-truth points. The proposed system ensures high positioning performance with fast sampling times and suggests a GPS waypoint system for user convenience. The centimeter-level precision GNSS information is provided at a 30Hz sampling rate, and the dead reckoning function ensures valid information even when passing through tall buildings and dense forests. The horizontal position error measured through the proposed system is 6.7cm, demonstrating a highly precise dynamic location measurement error within 10cm. The VRS network-RTK Linux system, which provides precise dynamic location information at a high sampling rate, supports a GPS waypoint planner function for user convenience, enabling easy destination setting based on GPS information.

• Annual Conference of KIPS
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• 2007.05a
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• pp.540-543
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• 2007

최근의 그리드 컴퓨팅 시스템 환경은 단일 환경에서 작게는 2~4CPU, 많게는 수백 CPU 이상의 시스템으로 구축되고 있고, 더욱이 지역적으로도 멀리 떨어져 있다. 따라서 이를 운용하는 기업에서는 시스템의 사용 현황을 신속하게 분석할 필요가 있다. 그러나 이렇게 혼재된 이 기종 및 컴퓨팅 환경하에서의 각 지역별 시스템 사용현황을 효과적으로 분석 한다는 것은 매우 어려운 일이다. 기존에 사용되어 온 그리드 컴퓨팅 시스템 환경에서의 사용율 관리 방법들은 Queueing 시스템이 가지고 있는 Accounting 분석 명령어로 text 형태의 Accounting raw data 의 결과를 추출하여 가공 처리하므로 데이터 증가 시 반응 속도가 현격하게 느려지는 상황이 발생한다. 또한 원격지 그리드 컴퓨팅 시스템 군의 사용율 분석은 데이터 분석 시 매번 원격지접근 절차를 사용하여 그리드 컴퓨팅 시스템 군에 접근한 후 해당 로컬 시스템 분석을 해야 하고 각 원격지시스템군별로 추출 된 데이터를 통합 관리해야 하는 문제점을 가지고 있다. 따라서 이러한 문제점을 해결하고자 하는 것이 본 논문에서 제안하는 3-tier 구조의 GSA(Grid System Account)이다. 제안한 GSA 는 각 원격지 별 데이터를 객체화하여 Database 에 저장 함으로써 데이터 분석 시 효과적으로 처리할 수 있으며, 다수의 원격지 그리드 컴퓨팅 시스템 군에 대한 복합적인 분석이 필요할 때 효율적으로 대처할 수 있다. 본 논문에서는 GSA 의 설계방법을 제안하고 구현하여 실 성능을 시험함으로써 보다 효율적인 그리드 컴퓨팅 시스템의 사용율 분석 관리가 가능함을 보였다.포는 감수성을 보이지 않았다. 따라서 위의 결과로부터 SLT-I에 감수성을 보이지 않은 Raw264.7세포를 대상으로 Gb3 발현 정도와 SLT-I의 세포독성의 관계를 규명하고자 Gb3의 발현을 증가시킨 후 SLT-I의 세포독성을 재차 평가하였다. 이 결과 $TNF-{\alpha}$의 처리에 의하여 6 hrs에 Gb3의 발현이 정점(43.5%)에 이르렀으며 36 hrs에 정상 수준(25.0%)으로 환원되었다. 그러나, Gb3의 발현이 증가함에도 불구하고 SLT-I의 세포독성에는 변화가 관찰되지 않았다. 따라서, SLT-I에 의한 세포독성은 세포의 종류에 따라서 다르며 또한, Gb3의 발현정도에만 의존적이지는 않을 것으로 생각된다. 이와 같은 결과는 E. coli 0157의 감염증 병인 연구에 있어 SLT-I과 Gb3의 발현의 상관관계에 대한 보다 심도 있는 연구가 필요함을 시사한다.만 분할률, 배반포 형성률 및 배반포의 세포수를 증가시키는 것으로 사료된다.수의 유출입 지점에 온도센서를 부착하여 냉각수의 온도를 측정하고 냉각수의 공급량과 대기의 온도 등을 측정하여 대사열의 발생을 추정할 수 있었다. 동시에 이를 이용하여 유가배양시 기질을 공급하는 공정변수로 사용하였다 [8]. 생물학적인 폐수처리장치인 활성 슬러지법에서 미생물의 활성을 측정하는 방법은 아직 그다지 개발되어있지 않다. 본 연구에서는 슬러지의 주 구성원이 미생물인 점에 착안하여 침전시 슬러지층과 상등액의 온도차를 측정하여 대사열량의 발생량을 측정하고 슬러지의 활성을 측정할 수 있는 방법을 개발하였다.enin과 Rhaponticin의 작용(作用)에 의(依)한 것이며, 이