• 한국해양학회지
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• v.21 no.1
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• pp.13-24
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• 1986

The daily net primary production by phytoplankton in the southeastern sea of Korea in October 1985 ranged from 0.7 to 2.7 gCm$\^$-2/ d$\^$-1/ and averaged to be 1.3 gCm$\^$-2/ d$\^$-1/. Surface total chlorophyll ranged from 0.97 to 3.59mg chlm$\^$-3/. Primary production by nano-phytoplankton(〈20$\mu\textrm{m}$) ranged from 43 to 97% in the surface layer. Optimum light intensity(Iopt)was around 300 to 700${\mu}$Es$\^$-1/m$\^$-1/. Surface primary production from 9:00 to 15:00 h was evidently inhibited by strong light intensity beyond the Iopt. Phytoplankton near the base of euphotic zone(30-40m) showed extremely low Iopt suggesting adaptation to a low light environment. Since Iopt represents the history of light experience of phytoplankton at a given depth, the extent of variation in I of phytoplankton at different depth seems to be related to the in tensity of turbulence mixing in the surface mixed layer. From the present study, ammonium excretion by macrozooplankton (〉350$\mu\textrm{m}$) contributes from 3 to 19% of daily total nitrogen requirement by phytoplandton in this area. Calculation of upward flux of nitrate to the surface mixed layer from the lower layer, based on the simple diffusion model, approximates 3% of nitrogen requirement by phytoplankton. However, large portion of nitrogen requirement by phytoplankton remains unexplained in this area. In upwelling area near the coast, adjective flux might be the major source for the nitrogen requirement by phytoplankton. This study suggests that the major nitrogen source for the phytoplankton growth might come from the pelagic regeneration by nano-and micro-sized heterotrophic plandkon. Enhancement of primary production during the passage of the warm Tsushima Current is discussed in relation with nutrient dynamics and hydrlgraphic processes in this area.

• Journal of Animal Environmental Science
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• v.15 no.3
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• pp.251-262
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• 2009

In this research dairy cattle barn facilities what are 4,198 houses hold over 50 heads were surveyed by scale and province. Full-time farms hold over 50 heads breed total 344,514 heads. Each of Farms holds 50 to 99 heads were 79.8 percent and breed average 82.1 heads. Dairy cattle barns were constructed september 1995 averagely. Each of barns have $1,740.0\;m^2$ scale. The construction type of dairy cattle barn was almost litter barn type 84.0%, freestyle type 5.1%, mooring+litter ground type 17.3% and other types 4.4%. The litter barn type was popular in small farms. But in large farms, freestyle type was popular than small farms. The construction type of dairy cattle barn was almost litter barn type 84.0%, freestyle type 5.1%, moohng+ltter ground type 17.3% and other types 4.4%. Type of dairy cattle robotic milking system was pipeline 41.5%, herringbone 22.8% and tandem 35.8%. The pipeline type was popular in small farms which have 50~99 heads. But in large farms which have over 200 heads, tandem type was popular than small farms. Proportion of floor type of dairy cattle barn was almost litter type 94.9%. Scraper type was popular in large farms than in small farms. Proportion of roof type of dairy cattle barn was slate 32.5%, vinyl 16.3%, sunlight 11.1%, panel 10.9, zinc plate 8.8 and steel plate 8.3%. Roof type was lots of slate type before 1995. But vinyl type is increasing after 1995. Proportion of wall type of dairy cattle barn was almost open type 83.3% and winch-curtain 26.8%. Utilization period of dairy cattle barn was 9.2 years about milker, 7.9 years about automatic feeder, 9.2 years about waterer and 10.4 years about electric facilities. In this results, there were lots of improvements about automatic feeder.

• The Korean Journal of Mycology
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• v.41 no.2
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• pp.104-111
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• 2013

Phellinus baumii is a medicinal mushroom used in Asian countries for a long period of time. The purpose of this study was to investigate the skin whitening activities of methanol extracts from fruiting bodies of P. baumii. To evaluate the antioxidant activities of the extract, polyphenol and flavonoid contents, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and chelating activity on ferrous ions were studied. For assay of skin whitening activities, tyrosinase and DOPA inhibitory activities, and tyrosinase and melanin synthesis inhibitory activities of B16/F10 melanoma cells treated with the methanol extract were investigated. The total polyphenol content of P. baumii methanol extract was 4.19. DPPH scavenging ability of P. baumii methanol extract was 88.26% in $25{\mu}g/mL$ concentration. We tested tyrosinase inhibitory activity and melanin contents in melanoma cells. The tyrosinase activity was inhibited to 65.17% at the concentration of $125{\mu}g/mL$ and melanin synthesis was inhibited to 57.61% at the concentration of $25{\mu}g/mL$. Overall, the experimental results showed that P. baumii methanol extract had inhibitory activities of tyrosinase and melanin synthesis by dose dependent manner in B16/F10 melanoma cells. Strong ultra-violet absorption spectra in the range of 270~370 nm indicated that ethanol extract of P. baumii could protect the skin from UV. Therefore, P. baumii methanol extract might be used for development of skin whitening, anti-UV and skin care agents.

• Journal of Life Science
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• v.17 no.12
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• pp.1660-1668
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• 2007

The non-ionic surfactant (NIS) Tween 80 was evaluated for its ability to influence invitro cumulative gas production, dry matter digestibility, cellulolytic enzyme activities, anaerobic microbial growth rates, and adhesion to substrates by mixed rumen microorganisms on rice straw, alfalfa hay, cellulose filter paper and tall fescue hay. The addition of NIS Tween 80 at a level of 0.05% increased significantly (P<0.05) in vitro DM digestibility, cumulative gas production, microbial growth rate and cellulolytic enzyme activity from all of substrates used in this study. In vitro cumulative gas production from the NIS-treated substrates; rice straw, alfalfa hay, filter paper and tall fescue hay was significantly (P<0.05) improved by 274.8, 235.2, 231.1 and 719.5% compared with the control, when substrates were incubated for 48 hr in vitro. The addition of 0.05% NIS Tween 80 to cultures growing on alfalfa hay resulted in a significant increase in CMCase (38.1%), xylanase (121.4%), Avicelase (not changed) and amylase (38.2%) activities after 36 h incubation. These results indicated that the addition of 0.05% Tween 80 could greatly stimulate the release of some kinds of cellulolytic enzymes without decreasing cell growth rate in contrast to trends reported with aerobic microorganism. Our SEM observation showed that NIS Tween. 80 did not influence the microbial adhesion to substrates used in the study. Present data clearly show that improved gas production, DM digestibility and cellulolytic enzyme activity by Tween 80 is not due to increased bacterial adhesion on the substrates.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.4
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• pp.385-390
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• 1999

HPLC method usually has been used for the determination of ATP and its related compounds in fish muscle and fish sauce. But, total amount of ATP related compounds in fish sauce is determined less than that of fish muscle. In order to establish the extract analysis method for ATP related compounds in fish sauce, a new enzymatic method was developed and compared with existing HPLC method. Fish sauce was extracted with chilled perchloric acid and neutralized to Ph 7.0 with potassium hydroxide solution, the extract was used as sample analyzed by HPLC as usual. On the other hand, for sample analyzed by enzymatic method, 1 ml extract solution was pipetted into test tube. To the tube, 0.5ml of mixed suspension adenosinedeaminase (4U), nucleosidephosphorylase (0.02U) and xanthineoxidase (0.03U) suspended in 2.0ml of 1/15 M sodium phosphate buffer solution pH 7.6 and 1.5ml deionized water wereadded for the decomposition of IMP, HxR and Hx to uric acid at $37^{\circ}C$ for 40 minutes. Total uric acid was determined by measuring optical density at 290nm. In HPLC method, salt decreased the total amount of ATP related compounds by $13.6\~16.2\%$ at $2.5\%$ concentration, but no effect in enzymatic method. IMP, HxR and Hx were detected at 254nm, while uric acid at only 290nm. The ratio of the total amount of ATP related compounds by HPLC method was about $45\%$ of that by enzymatic method in fish sauce. Form these results, enzymatic method is more accurate and simple than HPLC method for analysis of ATP related compounds in fish sauce.

• Applied Biological Chemistry
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• v.8
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• pp.87-93
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• 1967

1) For the micro-analysis of mercury in plant materials, the method of Furutani was shown to be the simplest and most efficient way and the recovery of the assay was about 98%. 2) When the rice grain was soaked in 1/1000 diluted solution of organo-mercury fungicide for 8 hours at the end of March, the amounts of mercury residues in the brown rice and unhulled rice were 8.8 to $9.5\;{\mu}g/g$ seeds and 10.1 to $10.7\;{\mu}g/g$ seeds, respectively. 3) By washing the treated rice seeds with running water for three days, tile residual mercury concentration was reduced to 1/4 to 1/5; thus the mercury residues were 1.86 to $1.92\;{\mu}g/g$ for brown rice and 1.96 to $2.93\;{\mu}g/g$ for unhulled rice. 4) The residual mercury was present more in the unhulled rice than in the brown rice, either before or after washing of the treated seeds. 5) Among the different rice varieties, no difference was observed in mercury residues by seed treatment and washing.

• The Korean Journal of Nuclear Medicine
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• v.29 no.1
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• pp.31-40
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• 1995

We compared stress/rest myocardial Tc-99m-MIBl tomographic image findings with rest/stress rubidium-82 tomographic images. In 23 patients with coronary artery disease (12 of them received bypass grafts before) and 6 normal subjects, rest rubidium PET study was performed : rubidium-82 and Tc-99m-MIBI were injected simultaneously to each patient after dipyridamole stress for rubidium PET and MIBI SPECT; and rest MIBI SPECT was performed 4 hours thereafter. We scored segmental decrease of rubidium or MIBI uptakes into 5 grades for 29 segments from 3 short-axis, vertical and horizontal slices. Scores were summed for each major arterial territory. When more score than two grade-2's or one grade-3 was considered as the cue for significant stenosis for major arterial territories, 67% of 46 stenosed arteries were found with MIBI studies and 78% of them by rubidium studies. Fourteen among 28 grafted arterial territories of 12 post-CABG patients were found normal with both rubidium and MIBI. Segmental scores were concordant between rubidium and MIBI in 72% of 709 stress segments and in 80% of 825 rest segments. Stress rubidium segmental scores were less than stress MIBI scores in 9%, so were rest rubidium scores. Stress rubidium scores were more than stress MIBI scores in 20% of segments, and rest rubidium segmental scores were more than rest MIBl scores in 11%. Rank correlations (Spearman's rho's more than 0.7(stress) and 0.5(rest), slopes (MIBI/rubidium) around 0.7(stress) and 0.9 (rest)) suggested deeper and wider defects in stress with rubidium. Slope over 1 (MIBI/rubidium) with LAD segemental scores at rest and 7 territories which had much larger score with MIBI revealed exaggeration of rest defects with rest MIBI in same-day stress/rest study. Difference scores (stress-rest for each territory) suggesting Ischemia were larger with rubidium (slope of MIBI/rubidium around 0.45). As has been implied by animal or separate-day-human studies, these segmental analyses with simultaneous examination in patients told that rubidium PET flow studies disclose ischemia more often than MIBI studies and that rest MIBI studies in same-day stress/rest-sequence gave a little larger rest defect than they would have shown.

• Journal of Animal Science and Technology
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• v.45 no.6
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• pp.923-932
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• 2003

This study was carried out to estimate the effects of breed and environment such as sex, test station, test year, test season, parity, initial and final weight on average daily gain, age at 90kg, backfat thickness, feed efficiency, lean percent and selection index on the basis of the performance data collected from 25,790 pigs of Duroc, Yorkshire and Landrace breeds which were performance-tested at the Korea Swine Testing Station from 1991 to 2002. The results obtained in the study are summarized as follows; 1. The means of the major economic traits were estimated as 959.95${\pm}$0.699g for average daily gain, 138.36${\pm}$0.072days for age at 90kg, 1.41${\pm}$0.001cm for backfat thickness, 2.33${\pm}$0.001 for feed efficiency, 56.71${\pm}$0.018% for lean percent and 221.65${\pm}$0.113 for selection index. 2. The effect of breed was statistically significant for all studied traits. Briefly, Duroc showed the best performance for the average daily gain and age at 90kg. Landrace had the best performances for the backfat thickness and lean meat percent. In feed efficiency and selection index, Yorkshire had a better score than other breeds. 3. The least-squares means of female and male for the traits studied were 923.05${\pm}$1.289g and 974.53${\pm}$0.856g for average daily gain, 139.74${\pm}$0.145days and 137.21${\pm}$0.097days for age at 90kg, 1.49${\pm}$0.002cm and 1.39${\pm}$0.002cm for backfat thickness, 2.43${\pm}$0.002 and 2.28${\pm}$0.002 for feed efficiency, 56.43${\pm}$0.034% and 56.81${\pm}$0.023% for lean percent and 211.37${\pm}$0.194 and 224.61${\pm}$0.129 for selection index. Therefore, males were superior to females for all traits examined. 4. The effect of test station was statistically significant for all traits except for selection index. Performances for age at 90kg, backfat thickness, feed efficiency and lean meat percent collected from Test station 2 were higher than those from Test station 1. However, Test station 1 showed better average daily gain. 5. The initial weight and final weight included as a covariate in this study had a significant influence on average daily gain, age at 90kg, backfat thickness, feed efficiency and selection index. From the absolute values of the estimated regression coefficients, it was inferred that the final weight had greater effect for the investigated traits than the initial weight.

• Korean Journal of Soil Science and Fertilizer
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• v.12 no.4
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• pp.169-178
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• 1980

Ammonium nitrate explosion technique was applied to seek a convenient method for the establishment of orchard on the undulating to rolling land or hill side of Pogog clay loam soil (Fine Aquic Fragiudalfs : Planosols) having high bulk density and low permeability. Explosions were made by three ammonium nitrate explosives placed in the bottom of 90cm deep auger hole with every 2m interval (Explosion I) and 4m interval (Explosion II) respectively. The effect of the explosion on physical properties of the soil was investigated and compared with the effect induced by manual digging, excavation of $1m{\times}1m$ in diameter and depth (Manual digging I) and trenching of $1m{\times}1m{\times}25m$ in width, depth, and length (Manual digging II) respectively. The results investigated after 7 months from the treatments are summarized as follows : 1. The explosion or manual digging reduced bulk density and hardness, whereas the treatments increased porosity, hydraulic conductivity, and available moisture-holding capacity of the soil. 2. The explosion of 4 m interval improved physical properties of the soil to optimum level up to 70cm of the distance from the explosion core in the range of depth 0-60cm, while in the case of depth from 60 to 100cm the optimum level was achieved only within 50cm radius. 3. When exploded in 2 m interval, the effect in the 0-60cm depth was overlapped between two explosion cores. The effect in the depth between 60 and 100cm, however, was found to be independent of the explosion intervals. 4. The manual digging was only costly and laborious but effective only within the work-up zone. 5. For the soils having bulk density higher than $1.4g/cm^3$ after the treatments, the field capacity determined 72 hours after a heavy rain was lower than the laboratory estimate at the suction of 1/3 atm. 6. The top growth of apple tree for the first year revealed that the explosion seemed better treatment than the manual digging, even though the difference was insignificant.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.11
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• pp.105-117
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• 1972

This experiment has been made to study the relationship between several characters affecting the field lodging and to establish some useful standards for selection of lodging resistant varieties and to classify the degree of lodging resistance in wheat and barley varieties of different sources. The experiment was carried at the Crop Experiment Station, Suwon in 1968. The results obtained are summarized as followings. a. The lodging index modified with bending moment of culm at breaking seemed to be the most useful character in checking the lodging resistance. Highly significant positive correlation (Barley; r=0.40-0.67, Wheat; r=0.46-0.68) was obtained between the lodging index and actual field lodging. b. Between two essential components expressing bending stiffness of the culm, the bending moment at breaking and secondary moment of inertia, a highly significant positive correlation (Barley; r=0.59, wheat; r=0.46-0.53) was observed. c. The bending stiffness of culm got stronger as the dry weight per unit culm, which express the quantity of accumulated dry matters in culm, increased. The correlation coefficient between those two factors was 0.35 to 0.40 in barley and 0.33 to 0.76 in wheat respectively. d. In both wheat and barley, highly significant negative correlation between lodging index and the other factors such as dry weight per unit culm (Barley; r=-0. 51 to -0.70, Wheat; r=-0.65 to -0.83) and bending moment of culm at breaking (Barley; r=-0.29 to -0.69, Wheat: r=-0.54 to -0.89) were observed. Particulary, weight of culm at breaking, secondary moment of inertia and section modulus showed significant negative correlation with lodging index in wheat. e. Outside diameter of culm expressed more intimate relationship with physical characteristics of culm than inside diameter and also showed highly significant correlation with weight of culm at breaking (Barley; r=0.42-0.56, Wheat; r=0.39-0.44) and with bending moment of culm at breaking (Barley; r=0.40-0.41, Wheat; r=0.38-0.49) and with secondary moment of inertia (Barley; r=0.56-0.57, Wheat; r=0.28-0.98) and with section modulus (Wheat; r=0.22-0.96). Between the thickness of culm and physical characteristics of culm also showed the positive correlation. f. There was positive correlation between the culm length and actual field lodging in several groups of variety among the varieties tested. But the culm length seemed to undesirable as a selection measure for the selection of the lodging resistant variety considering the stiffness of culm. g. In classification of lodging resistance for the varieties tested, many Korean barley varieties expressed excellent lodging resistant than wheat, but most of the wheat and barley varieties from Japan considered quite resistant to lodging. h. In selection of lodging resistant varieties, lodging index lower than 1.67 in barley and 1. 76 in wheat considered highly resistant to actual field lodging.