Recently, exhaust gas regulation has been gradually strengthened due to depletion of fossil fuels and environmental problem like a global warming. Due to this global problem, the demand for eco-friendly vehicle development is rapidly increasing. A clean diesel vehicle is considered as a realistic alternative. The common-rail fuel injection system, which is the key technology of the clean diesel vehicle, has adopted injection strategies such as high pressure injection, multiple injection for better atomization of the fuel. In addition, the emission regulations in the future is expected to be more stringent, which a conventional engine is difficult to deal with. One of the way for actively proceeding is the study of alternative fuels. Among them, the bio-diesel has been attracted as an alternative of diesel. So, in this study, spray characteristics of bio-diesel was analyzed in the common-rail fuel injection system with three injectors driven by different operating mechanism.