• Korean Journal of Breeding Science
• /
• v.42 no.5
• /
• pp.540-546
• /
• 2010

This study was conducted to understand the role of soybean pathogenesis-related 10 (GmPR10) gene in salt tolerance and to develop salt-tolerant rice using GmPR10 cDNA. GmPR10 transgene was expressed constitutively in the shoot and root of the $T_1$ transgenic rice plants. Interestingly, however, the levels of the transgene expression were increased temporally up to over four- to five-fold in the shoot and root by 125 mM NaCl treatment, peaking at six hours after the treatment and decreasing thereafter. Electrolyte leakage of leaf cells under 125 mM NaCl treatment was lower in all the transgenic lines than in the control variety, Dongjin-byeo. Ability of seedlings to recover from 125 mM NaCl treatment for two weeks was higher in the transgenic plants than in the control plants. These results demonstrated that GmPR10 had function to increase cell integrity and promote growth under the saline stress imposed by NaCl. The transgenic line GmPR10-3 which showed highest ability to recover from the saline stress could be used as a potential source for salt tolerance in rice breeding programs.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.26 no.2
• /
• pp.83-90
• /
• 2006

To develop transgenic birdsfoot trefoil (Lotus corniculatus L.) plants tolerant to environmental stress, Arabidopsis NDPK gene (AtNDPK) was introduced into birdsfoot trefoil plants using Agrobacterium-mediated transformation and expressed powerfully under the control of the E35S promoter. The expression vector, pEN-K was used for introduction of AtNDPK gene into birdsfoot trefoil plaits. The transformed calli were selected on kanamycin containing medium and then regenerated. The transformed birdsfoot trefoil plants were cultivated for 4 months on BOi2Y medium. Genomic DNA PCR and Southern blot analysis confirmed the incorporation of AtNDPK into the birdsfoot trefoil genome.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.5 no.2
• /
• pp.25-33
• /
• 2002

This study was carried out to select the utilizable vegetations for the stabilization of slopes and river banks, landscape creation and water quality cleansing in dams, lakes and streams. In this study, 10 herbaceous plants were investigated for their survival and growth characteristics under complete and partly flooding conditions in the nursery. The ranking order of flooding tolerance based on survival ability showed that Iris pseudoacorus, Phragmites communis and Phragmites japonica were first, Typha angustata, Miscanthus sacchariflorus and Pennisetum alopecuroides were second, and Cymbopogon tortilis, Miscanthus sinensis, Kummerowia striata and Lolium multiflorum were last. That of flooding tolerance base on growth ability showed that Iris pseudoacorus, Phragmites communis, Phragmites japonica, Typha angustata and Miscanthus sacchariflorus were first, Pennisetum alopecuroides, Miscanthus sinensis and Kummerowia striata were second, and Cymbopogon tortilis and Lolium multiflorum were last. Espcially, Miscanthus sacchariflorus, Iris pseudoacorus, Phragmites japonica and Phragmites communis showed good survival and growth abilities when they were complete flooded for 60 days. Also high flooding tolerant species grow as better as flooding period longer under the partly flooding condition. It was found that Pennisetum alopecuroides and Kummerowia striata have excess moisture tolerance because they showed good survival and growth under the partly flooding condition. This study confirmed that the possibilities of utilizing Iris pseudoacorus, Typha angustata, Phragmites japonica, Phragmites japonica, Phragmites communis, Miscanthus sacchariflorus, Pennisetum alopecuroides and Kummerowia striata as the efficient plants for vegetation measures on the flooded slopes of dam and various impoundment sites.

• Journal of Plant Biotechnology
• /
• v.47 no.4
• /
• pp.324-329
• /
• 2020

Reed (Phragmites spp.) is a rhizomatous plant of the Poaceae family and is known as high tolerant plant to heavy metal contaminants. This plant is widely recognized as a Cd root-accumulator, but improved heavy metal tolerance and uptake capacity are still required for phytoremediation efficiency. To enhance capacity of hyperaccumulator plants, ethyl methane sulfonate (EMS) as chemical mutagen has been introduced and applied to remediation approaches. This study aimed to select EMS-mutagenized reeds representing high Cd resistance and large biomass and to investigate their ability of Cd accumulation. After 6 months cultivation of M₂ mutant reeds under Cd stress conditions (up to 1,500 µM), we discovered seven mutant individuals that showed good performances like survivorship, vitality, and high accumulation of Cd, particularly in their roots. Compared to wild type (WT) reeds as control, on average, dry weight of mutant type (MT) reeds was larger by 2 and 1.5 times in roots and shoots, respectively. In addition, these mutant plants accumulated 6 times more Cd, mostly in the roots. In particular, MT8 reeds showed the greatest ability to accumulate Cd. These results suggest that EMS mutagenesis could generate hyperaccumulator plants with enhanced Cd tolerance and biomass, thereby contributing to improvement of phytoremediation efficiency in Cd-contaminated soil or wastewater. Further studies should focus on identifying Cd tolerance mechanisms of such EMS-mutagenized plants, developing techniques for its biomass production, and investigating the practical potential of the EMS mutants for phytoremediation.

• Korean Journal of Food Science and Technology
• /
• v.52 no.1
• /
• pp.40-45
• /
• 2020

Over 500 genetically modified organisms (GMOs) have been developed since 1996, of which nearly 44% have glufosinate herbicide-tolerant traits. Identification of specific markers that can be used to identify herbicide-tolerant traits is challenging as the DNA sequences of the gene(s) of a trait are highly variable depending on the origin of the gene(s), plant species, and developers. To develop specific PCR marker(s) for the detection of the glufosinate-tolerance trait, DNA sequences of several pat or bar genes were compared and a diverse combination of PCR primer sets were examined using certified reference materials or transgenic plants. Based on both the qualitative and quantitative PCR tests, a primer set specific for pat and non-specific for bar was developed. Additionally, a set of markers that can detect both pat and bar was developed, and the quantitative PCR data indicated that the primer pairs were sensitive enough to detect 0.1% of the mixed seed content rate.

• Korean Journal of Weed Science
• /
• v.15 no.3
• /
• pp.224-231
• /
• 1995

Paraquat, the representative bipyridilium herbicide, has high phytotoxic activity through generating toxic oxygen species such as superoxide, hydrogen peroxide and hydroxy radical. The response patterns of plants to paraquat were various. It was assumed that the different response was derived from different antioxidative mechanisms including antioxidative enzymes and antioxidant. Paraquat treatment increased reducing sugar content and malondialdehyde formation at 35 days after treatment in a dose-dependent manner but chlorophyll content decreased. Glutathione content increased by paraquat treatment and tolerant species showed more glutathione content than susceptible species. Superoxide dismutase activity increased with the increase in paraquat concentration and that was higher in tolerant species than susceptible species. Photosynthetic activity(PSII activity) was affected by paraquat, so the susceptible species showed more reduced oxygen evolving capacity than tolerant species. Catalse, NADPH-cytochrome C reductase, and malate dehydrogenase, the enzymes tested in this study, showed that the activities decreased by paraquat treatment. Further studies are necessary to determine whether antioxidative system cause the tolerance to paraquat.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.29 no.4
• /
• pp.299-306
• /
• 2009

Oxidative stress is the main limiting factor in crop productivity. To solve global environmental problems using the plant biotechnology, we have developed on the oxidative stress-tolerant transgenic tall fescue plants via Agrobacterium-mediated genetic transformation method. In order to develop transgenic tall fescue (Festuca arundinacea Schreb.) plants with enhanced tolerance to multiple environmental stresses, nucleotide diphosphate kinase gene under the control of CaMV35S promoter were introduced into genome of tall fescue plants. Proteomic analysis revealed that transgenic tall fescue not only accumulated NDP kinase 2 protein in their cells, but also induced several other antioxindative enzyme-related proteins. When leaf discs of transgenic plants were subjected to cold stress, they showed approximately 30% less damage than wild-type plants. In addition, transgenic tall fescue plants showed normal growth when transgenic plants were subjected to $4^{\circ}C$ for 3 days treatments. These results suggest that transgene is important in ROS scavenging by induction of antioxidative proteins, and could improve abiotic stress tolerance in transgenic tall fescue plants.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.24 no.1
• /
• pp.21-24
• /
• 2004

To determine lethal temperature of alfalfa (Medicago sativa L. cv Vernal) at heat-stressed conditions, seedlings grown in a small pots for 4 weeks were subjected to different temperature regimes of heat treatment. No apparent demage was observed when the plants were treated at 45, 50 or $60^{\circ}C$ for 1 h. Heat treatments at 60 and $65^{\circ}C$ for 1 h, several plants were withered and showed damage symptom on their leaves. When the plants were exposed to $70^{\circ}C$ for 1 h, most of leaves were severely withered, but it was not lethal conditions for the whole plants. By contrast, most of plants were died within one day after heat treatment at $80^{\circ}C$ for 1h. Furthermore, plants exposed to $80^{\circ}C$ for 50 min were also died within 7 days. It was found that new shoots were regenerated from the plants that had been treated at $80^{\circ}C$ within 45 min. These results indicate that heat treatment at $80^{\circ}C$ for 50 min is an optimum condition to distinguish the lethality of alfalfa plants. Simple viability assay system established in this study will be useful fer selection and characterization of heat-tolerant transgenic alfalfa plants.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.44 no.4
• /
• pp.309-315
• /
• 1999

Rice seedling test was conducted to check the loging tolerance at ripening stage through evaluating the root characters. Thirteen Korean and foreign rice cultivars with direct seeding adaptable or high quality characteristics were grown in a cell pot and under submerged paddy. The root characters and pushing resistance of rice hill were determined at seedling and ripening stage, respectively. The diameter of crown root at the 7th and 8th leaf stages was thicker in lodging tolerance cultivars than those of others and showed significant-positive correlation with both pushing resistance and crown root diameter of mature plants. Also, the tensile strength of crown root at the 7th and 8th leaf stage showed highly positive correlation with the tensile strength of crown root of mature plants. The number of crown root at 7th leaf stage was significant-positively correlated with that of mature plant. The diameter of seminal root was not significantly correlated with the diameter of crown root throughout the whole growth stage. These results indicate that the diameter, tensile strength and number of crown root associated with root lodging tolerance can be detected with the seedling at about 7th or 8th leaf stage, and the seedling test using the cell pot is an useful and practical method to select lodging tolerant cultivars or lines of rice based on root characters, especially diameter of crown root.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.50 no.4
• /
• pp.276-280
• /
• 2005

Low temperature is one of the most severe abiotic stress factors limiting growth, productivity and distribution of winter cereals. Reliable field screening method, which can detect small differences in winter survival, is important for the effective selection and development of plants to identity superior cold tolerant winter cereal genotypes. This study was undertaken to provide improved screening method of winter hardiness in the field by increasing the accuracy in evaluating winter hardiness of barley (Hordeum vulgare L.). We introduced furrow in field screening of winter survival. By sowing the plants at the ridge and base, we could minimize the effect of topographic variation in a field by giving higher and lower level of stress at the same time. This method could be used by breeders to conduct accurate evaluation of winter hardiness by selecting the better treatment, which shows close to normal distribution, among the winter survival rate from the ridge, base and mean survival rate of the two in a screening field.