• Title/Summary/Keyword: stomata length

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Effect of Shading Level of Nursing Bed on the Shoot Growth of Rooted Cuttings in Native Hydrangea serrata for. acuminata (육묘상의 차광정도가 자생 산수국 삽목묘의 신초생장에 미치는 영향)

  • Lee, Seung Youn;Lee, Sung Chun;Park, Sung Tae;Rhee, Jung Chul;Lee, Taek Joo;Kim, Kwang Jin;Lee, Jeong Sik
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.3
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    • pp.153-160
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    • 2008
  • This experiment was conducted to find out an adequate shading level (0, 55, 74, and 95%) on the shoot growth of rooted cuttings of native Hydrangea serrata for. acuminata. New shoot height (NSH), length and width of leaf, and stem diameter under 55% shading were the greatest. Net photosynthetic rate under 55% shading was the greatest, while intercellular $CO_2$ concentration was the least in this treatment. The greatest content of photosynthetic pigments (total chlorophyll, carotenoid) was observed in leaves grown under a 74%, followed by a 55% shading level. Leaf stomata were observed on the abaxial surface, but those on adaxial surface in all shading treatments were not observed. In 95% shading, decreased stomatal density and development were observed. Higher stomatal density and development, however, were observed in 55% shading. Therefore, it is concluded that a 55% shading level was the optimum for growth of new shoots of rooted cuttings of native H. serrata for. acuminata.

A Morphological and Anatomical Study on the Leaves of the Genus Acer in Korea (한국산(韓國産) 단풍나무속(屬)의 잎의 형태(形態) 및 해부학적(解剖學的) 연구(硏究))

  • Park, Kwang Woo;Kim, Sam Sik
    • Journal of Korean Society of Forest Science
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    • v.64 no.1
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    • pp.52-63
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    • 1984
  • This study was intended to identify 17 taxa. (5 varieties and 12 species) of the genus Acer in Korea on the basis of the shapes of stomata, the type of trichome on the different part of leaves, the shapes, arrangement and number of stele in cross section of petiole. The results obtained were summarized as follows; 11 The shape of guard cells of stomata in the genus Acer was anomcytical, and the size of the cells ranged from 10.25 to $21.00{\mu}$ in length and from 7.57 to $11.83{\mu}$ in width. 2) Eleven types of trichome on the leaf in the genus Acer were found; pilose, sericeous, velutinous, woolly, glabrate, puberulent, bladder hair, hispid, hirsute and uncinate. This characteristics also established a good criterion for identification of species. 3) The stele of petiole in the genus Acer was characterized by eustele and atactostele with polybranch, and the six groups of the shape of numerical change of stele; B>M=T, BM

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The Effects of Indole Acetic Acid (IAA) on Cd$^{2+}$_induced Physiological Toxicities in Commelina communis L. (Cd$^{2+}$ 에 의한 닭의장풀의 식물 독성에 Indole acetic acid가 미치는 영향)

  • 이준상
    • Korean Journal of Environmental Biology
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    • v.21 no.3
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    • pp.234-239
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    • 2003
  • 3-weeks old Commelina was transferred to and grown in Hoagland solution (($\pm 100 \mu M \;Cd^{2+}, 100 \mu M \;Cd^{2+}+10 \mu M\;IAA,100 \mu M\; Cd^{2+}+100 \mu M \; IAA,100 \mu M \; Cd^{2+} +1 \mu M \; IAA)$) for three weeks and then a number of physiological activities was investigated. In the control the length of stem was increased to 7 cm after 3 weeks, but in the treatment of $Cd^{2+}$, 2.0 cm was grown. In cases of IAA with $Cd^{2+}$, the growth of the plants was increased to 3.7 cm, 5.0 cm and 3.3 cm in 100 $\mu$M, 10 $\mu$M and 1 mM IAA respectively, Cadmium stimulated stomatal opening. The stomata, treated with $Cd^{2+}$ opened to n degree of about 6.1 $\mu\textrm{m}$, but the stomata, treated with no cadmium opened to 4.5 $\mu\textrm{m}$. In the treatment of various concentation of IAA with cadmium the stomata opend to about 1 $\mu\textrm{m}$ more than that of cadmium alone. Cadmium reduced total chlorophyll content up to 13% for 3 weeks. In the treatment of $Cd^{2+}$ + IAA (10 $\mu$M and 100 $\mu$M) the clear change of total chlorophyll content was not observed, but in the addition of 1 mM IAA to $Cd^{2+}$ reduced the total chlorophyll content to about 9%. $Cd^{2+}$ reduced to 9% and 11% of Fv/Fm after two and three weeks respectively. In cases of IAA with $Cd^{2+}$, there were no clear changes of Fv/Fm. Cadmium reduced water potential to 67% after 3 weeks incubation, but in cases of IAA with $Cd^{2+}$, water potential was not clearly changed. Therefore, it could be concluded that the treatment of IAA showed clear alleviation of $Cd^{2+}$-induced several physiological toxicities.

Studies on a Tetraploid of Robinia pseudoacacia L. Selected in the Planted Forest (조림지(造林地)에서 선발(選拔)한 자연(自然) 4배체(倍體) 아까시나무(Robinia pseudoacacia L.)에 관(關)한 연구(硏究))

  • Kim, Chung Suk;Lee, Suk Koo
    • Journal of Korean Society of Forest Science
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    • v.14 no.1
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    • pp.39-44
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    • 1972
  • Cytological investigation was done on a tetraploid tree of Robinia pseudoacacia L. selected in the planted forest in Chilbo Mountain. From the results of observation of chromosomes on mitotic metaphase in leaves, it was confirmed that the mutant is tetraploid plant (2n=40). The external form of leaves of the spontaneous tetraploid was gigantic and dark green one in comparison with diploid. There was no difference in the size of guard cell and the number of stomata per unit area between the mutant and the diploid plant, however, length and thickness of wood fiber were increased as compared with that of diploid. The spontaneous gigas tetraploid showed growth performance as much as the colchicine induced gigas tetraploid.

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The Variation of Natural Population of Pinus densiflora S. et Z. in Korea(II) -Characteristics of Needle and Wood of Myong-Ju, Ul-Jin, and Suweon Populations- (소나무천연집단(天然集團)의 변이(變異)에 관(關)한 연구(硏究)(II) -명주(溟州), 울진(蔚珍), 수원집단(水原集團)의 침엽(針葉) 및 재질형질(材質形質)-)

  • Yim, Kyong Bin;Kwon, Ki Won
    • Journal of Korean Society of Forest Science
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    • v.31 no.1
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    • pp.8-20
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    • 1976
  • For study on the variation of natural stand, three populations of Pinus densiflora S. et Z. were selected at samsanri Yongogmyun Myongjugun Kangwondo (4), Hawonri seomyun Uljingun Kyongbuk (5), and Emogdong Suweon Kyongkido (6) successibely after the selection of three population in 1974. Twenty individual trees were chosen from each population and the morphological characteristics of trees, needle and wood properties were investigated on the trees. The results are summerized as follows; 1. Serration density, resin canal number in needle did not show significant differences, however stomata row number in the both sides of needle showed highly significant differences among 3 populations. But significant differences were calculated among individual trees in a population regarding any character of needles. 2. Ail population had high correlation on the stomata row between abaxial and adaxial side of needle. 3. The Myongjungun population showed the highest value of resin duct index, which means the population had the highest degree of hybrid character. 4. The ring segment width and summerwood percentage in the wood properties had significant differences, and yet specific gravity and tracheid length had not significant differences statistically among 3 populations. But all the values were significant statistically among the ring segments within population. 5. The ring segment width decreased rapidly with increasing tree age but summerwood percentage, specific gravity, tracheid length increased slowly to the middle age of tree and then decreased slowly after the age. But the patterns of decrease or increase were some different by population. 6. The values of Uljingun population were generally high in the coefficient of variation on all the needle characters. And the values of Suweon population were always the highest and those of Myongjugun population the lowest in the coefficient of variation on all the wood properties.

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The Variation of Natural Population of Pinus densiflora S. et Z. in Korea (VI) - Genetic Variation of the Progency Originated from Myong-Ju, Ul-Jin and Suweon Populations - (소나무 천연집단(天然集團)의 변이(變異)에 관(關)한 연구(硏究)(VI) - 명주(溟洲), 울진(蔚珍), 수원(水原) 소나무 집단(集團)의 차대(次代)의 유전변이(遺傳變異) -)

  • Yim, Kyong Bin;Kwon, Ki Won;Lee, Kyong Jae
    • Journal of Korean Society of Forest Science
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    • v.38 no.1
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    • pp.33-45
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    • 1978
  • The purpose of present study is to analyze the genetic variation of natural stand of Pinus densiflora. In 1975 following after the selection of 1974, twenty trees from each of three natural populations of the species were selected and their open-pollinated seeds were collected, and the locations and conditions of the populations ate presented in table 1, 2 and figure 1. Some morphological traits of the populations were already detailed in our second report of this series, in which Myong-Ju and Ul-Jin populations were regarded to be superior phenotypically to suweon population. The morphological traits of cone, seed and seed-wing, and also the growth performances and needle characters of the seedling were observed in the present study according to the previous methods. The results obtained are summarized as follows; 1. The meteorological data obtained by averaging the records of 30 year period (1931~1960) measured from the nearest meteorological stations to each population are shown in fig.2, 3, 4. The distributional patterns of investigated climate factors are generally considered to be similar among the locations. However, the precipitation density during growing season and the air temperature during dormant season on Suweon area, population 6, were quite different from those of the other areas. 2. The measurements of fresh cone weight, length, diameter and cone index, i.e., length to diameter ratio are presented in table 7. As shown in table 7, all these traits except for cone diameter seem to be highly significant in population differences and family differences within population. 3. The morphological traits of seed and seed-wing are detailed in table 8, 9, and highly significant differences are recognized among the populations and the families within population in seed-wing length, seed-wing index, seed weight, seed-length and seed index but not among the populations in the other observed traits. The values of correlation coefficient between the characters of cone and seed are given in table 10 and the positive significant correlations can be observed in the most parts of the compared traits. 4. Significant statistical differences among populations and families within population are observed in the growth performances of 1-0 and 1-1 seedling height of these progenies. But the differences in root collar diameter are shown only among families within population. As shown in table 13, the most parts of correlations are not significant statistically between the growth performances of seedling and the seed characters. 5. The number of stomata row on both sides of needle and the serration density were measured in the seedlings from each of the families of the three populations. As shown in table 15, statistical differences are considered to be significant among the populations and among the families within population in serration density but not among the populations in stomata row on both sides of the needle. The results differ from those of the third report of this series. Even if one of the reason seems to be the diversity of selected populations, it could not be confirmed definitely. The correlations between progenies and parents are not generally observed in the investigated traits of needle as shown in table 16.

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The Variation of Needle Characteristics of Picea jezoensis(Siebold & Zucc.) Carriere Populations in Korea (한국산 가문비나무 자생집단의 침엽특성 변이)

  • Song, Jeong-Ho;Hur, Seong-Doo;Kang, Kyu-Suk;Yang, Byeung-Hoon;Lee, Jung-Joo
    • Korean Journal of Plant Resources
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    • v.22 no.1
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    • pp.31-36
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    • 2009
  • The aim of this study was to examine geographic variation to support a genetic resource conservation plan of rare endemic Picea jezoensis (Siebold & Zucc.) Carriere. Three populations of P. jezoensis were analysed using multivariate analysis for 7 characteristics of needle morphology. In the morphological characters, seven characters of needle were 15.11 mm (needle length), 1.43 mm (needle width), 0.42 mm (needle thickness), 10.9 (length / width of needle), 3.6 (width / thickness of needle), 0.47 mm (Distance between resin duct and vascular), 17.4 ea. (number of stomata row), respectively. Nested analysis showed that were statistically significant differences among populations as well as among individuals within populations in 6 quantitative characters. In 5 of 7 characters, variance components among populations were higher than those among individuals within populations. Cluster analysis using complete linkage method showed two groups (Mt. Gyebang populations was genetically distinct from the other two populations) to Euclidean distance 0.4. Among principal components, primary 2 principal components appeared to be major variables because of the loading contribution of 100%.

Taxonomic Examination of Isoëtes L. (Isoëtaceae) in South Korea (우리나라 물부추속 (물부추과)에 대한 분류학적 고찰)

  • Jung, Jongduk;Kim, Changkyun;Kim, Hojun;Choi, Hong-Keun
    • Korean Journal of Plant Taxonomy
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    • v.39 no.2
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    • pp.63-73
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    • 2009
  • $Iso{\ddot{e}}tes$ L. ($Iso{\ddot{e}}taceae$) is difficult to identify among the infrageneric taxa because of morphological simplicity. Since two species (I. japonica and I. coreana) had been reported, I. jejuensis and I. hallasanensis were described as two new species from Jeju Island, South Korea. The habitats of all species were confirmed by current studies except for I. japonica. We compared the morphological and anatomical characters of seven species, three domestic species and four species in adjacent regions (I. taiwanensis, I. sinensis, I. japonica, and I. asiatica). I. asiatica differs from other species in the lobe number of corm, spreading sporophylls, lack of stomata and degree of velum development. All of the seven species were identified by the characteristics of their mega- and microspores. Especially, it was possible to identify the four species in South Korea by the types of ornamentation on their megaspores. Each species has a specific type of megaspore ornamentation: I. coreana - cristate, I. jejuensis - rugulate, I. hallasanensis - echinate, and I. japonica - reticulate. We also discussed the phylogeny of the genus in East Asia in relation to recent studies based on the second intron of LEAFY sequence and amplified fragment length polymorphism (AFLP) analysis. We suggest strategies for the conservation of the endangered $Iso{\ddot{e}}tes$ species in South Korea.

The Variation of Natural Population of Pinus densiflora S. et Z. in Korea (III) -Genetic Variation of the Progeny Originated from Mt. Chu-wang, An-Myon Island and Mt. O-Dae Populations- (소나무 천연집단(天然集團)의 변이(變異)에 관(關)한 연구(硏究)(III) -주왕산(周王山), 안면도(安眠島), 오대산(五臺山) 소나무집단(集團)의 차대(次代)의 유전변이(遺傳變異)-)

  • Yim, Kyong Bin;Kwon, Ki Won
    • Journal of Korean Society of Forest Science
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    • v.32 no.1
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    • pp.36-63
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    • 1976
  • The purpose of this study is to elucidate the genetic variation of the natural forest of Pinus densiflora. Three natural populations of the species, which are considered to be superior quality phenotypically, were selected. The locations and conditions of the populations are shown in table 1 and 2. The morphological traits of tree and needle and some other characteristics were presented already in our first report of this series in which population and family differences according to observed characteristics were statistically analyzed. Twenty trees were sampled from each populations, i.e., 60 trees in total. During the autumn of 1974, matured cones were collected from each tree and open-pollinated seeds were extracted in laboratory. Immediately after cone collection, in closed condition, the morphological characteristics were measured. Seed and seed-wing dimensions were also studied. In the spring of 1975, the seeds were sown in the experimental tree nursery located in Suweon. And in the April of 1976, the 1-0 seedlings were transplanted according to the predetermined experimental design, randomized block design with three replications. Because of cone setting condition. the number of family from which progenies were raised by populations were not equal. The numbers of family were 20 in population 1. 18 in population 2 and 15 in population 3. Then, each randomized block contained seedlings of 53 families from 3 populations. The present paper is mainly concerned with the variation of some characteristics of cone, seed, needle, growth performance of seedlings, and chlorophyll and monoterpene compositions of needles. The results obtained are summerized as follows. 1. The meteorological data obtained by averaging the records of 30 year period, observed from the nearest station to each location of populations, are shown in Fig. 3, 4, and 5. The distributional pattern of monthly precipitation are quite similar among locations. However, the precipitation density on population 2, Seosan area, during growing season is lower as compared to the other two populations. Population 1. Cheong-song area, and population 3, Pyong-chang area, are located in inland, but population 2 in the western seacoast. The differences on the average monthly air temperatures and the average monthly lowest temperatures among populations can hardly be found. 2. Available information on the each mother trees (families) studied, such as age, stem height, diameter at breast height, clear-bole-length, crown conditions and others are shown in table 6,7, and 8. 3. The measurements of fresh cone weight, length and the widest diameter of cone are given in Tab]e 9. All these traits arc concerned with the highly significant population differences and family differences within population. And the population difference was also found in the cone-index, that is, length-diameter ratio. 4. Seed-wing length and seed-wing width showed the population differences, and the family differences were also found in both characteristics. Not discussed in this paper, however, seed-wing colours and their shapes indicate the specificity which is inherent to individual trees as shown in photo 3 on page 50. The colour and shape are fully the expression of genetic make up of mother tree. The little variations on these traits are resulted from this reason. The significant differences among populations and among families were found in those characteristics, such as 1000-seed weight, seed length, seed width, and seed thickness as shown in table 11. As to all these dimensions, the values arc always larger in population 1 which is younger in age than that of the other two. The population differences evaluated by cone, seed and seed-wing sizes could partly be attributed to the growth vigorousity. 5. The values of correlation between the characteristics of cone and seed are presented in table 12. As shown, the positive correlations between cone diameter and seed-wing width were calculated in all populations studied. The correlation between seed-wing length and seed length was significantly positive in population 1 and 3 but not in population 2, that is, the r-value is so small as 0.002. in the latter. The correlation between cone length and seed-wing length was highly significant in population 1, but not in population 2. 6. Differences among progenies in growth performances, such as 1-0 and 1-1 seedling height and root collar diameter were highly singificant among populations as well as families within population(Table 13.) 7. The heritability values in narrow sense of population characteristics were estimated on the basis of variance components. The values based on seedling height at each age stage of 1-1 and 1-0 ranged from 0.146 to 0.288 and the values of root collar diameter from 0.060 to 0.130. (Table 14). These heritability values varied according to characteristics and seedling ages. Here what must be stated is that, for calculation of heritability values, the variance values of population was divided by the variance value of environment (error) and family and population. The present authors want to add the heritability values based on family level in the coming report. It might be considered that if the tree age is increased in furture, the heritability value is supposed to be altered or lowered. Examining the heritability values studied previously by many authors, in pine group at age of 7 to 15, the values of height growth ranged from 0.2 to 0.4 in general. The values we obtained are further below than these. 8. The correlation between seedling growth and seed characteristics were examined and the values resulted are shown in table 16. Contrary to our hypothetical premise of positive correlation between 1-0 seedling height and seed weight, non-significance on it was found. However, 1-0 seedling height correlated positively with seed length. And significant correlations between 1-0 and 1-1 seedling height are calculated. 9. The numbers of stomata row calculated separately by abaxial and adaxial side showed highly significant differences among populations, but not in serration density. On serration density, the differences among families within population were highly significant. (Table 17) A fact must be noted is that the correlation between stomata row on abaxial side and adaxial side was highly significant in all populations. Non-significances of correlation coefficient between progenies and parents regarding to stomata row on abaxial side were shown in all populations studied.(Table 18). 10. The contents of chhlorophyll b of the needle were a little more than that of chlorophyll a irrespective of the populations examined. The differences of chlorophyll a, b and a plus b contents were highly significant but not among families within populations as shown in table 20. The contents of chlorophyll a and b are presented by individual trees of each populations in table 21. 11. The occurrence of monoterpene components was examined by gas liquid chromatography (Shimazu, GC-1C type) to evaluate the population difference. There are some papers reporting the chemical geography of pines basing upon monoterpene composition. The number of populations studied here is not enough to state this problem. The kinds of monoterpene observed in needle were ${\alpha}$-pinene, camphene, ${\beta}$-pinene, myrcene, limonene, ${\beta}$-phellandrene and terpinolene plus two unknowns. In analysis of monoterpene composition, the number of sample trees varied with population, I.e., 18 families for population 1, 15 for population 2 and 11 for population3. (Table 22, 23 and 24). The histograms(Fig. 6) of 7 components of monoterpene by population show noticeably higher percentages of ${\alpha}$-pinene irrespective of population and ${\beta}$-phellandrene in the next order. The minor Pinus densiflora monoterpene composition of camphene, myrcene, limonene and terpinolene made up less than 10 percent of the portion in general. The average coefficients of variation of ${\alpha}$-pinene and ${\beta}$-phellandrene were 11 percent. On the contrary to this, the average coefficients of variation of camphene, limonene and terpinolene varied from 20 to 30 percent. And the significant differences between populaiton were observed only in myrcene and ${\beta}$-phellandrene. (Table 25).

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Radiation Effect of X-Ray and Thermal Neutron on Robinia pseudoacacia L. and Some Other Species (아까시나무외 몇 수종(樹種)에 대(對)한 X-Ray와 Thermal Neutron의 조사효과(照射効果))

  • Kim, Chung Suk;Lee, Suk Koo;Hyun, Sin Kyu
    • Journal of Korean Society of Forest Science
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    • v.17 no.1
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    • pp.1-15
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    • 1973
  • In an effort to improve the major tree species in Korea, the seed of Robinia pseudoacacia, Pinus rigida, Pinus densiflora, Pinus thunbergii and Larix leptolepis were treated with X-ray and thermal neutron at the Brookhaven National Laboratory, and germination rate of the seed and some characteristics of the seedlings from irradiated seed were investigated and the results were summarized as follows. 1. The germination rate of the irradiated seed of Robinia pseudoacacia, Pinus densiflora, Pinus thunbergii and Pinus rigida was decreased, when the irradiation time of thermal neutron increased from 3 hours to 9 hours. The seed of Larix leptolepis was completely died out in all range of irradiation time. 2. The seed of Pinus densiflora, Robinia pseudoacacia and Pinus rigida showed low germination rate, when the dosage of radiation increased in the range of 10,000r-30,000r X-ray. This dosage of radiation was almost lethal to the seed of Pinus thunbergii and Larix leptolepis. 3. The growth rate of radiated Robinia pseudoacacia has been decreased when the dosage of X-ray and thermal neutron increased. However, the trees treated with thermal neutron for 3 hours showed 14.9 percent-increase in seedling height and some thornless individuals appeared in this treatment. 4. Individuals with variegated leaf, rugose leaf and albino were appeared in X-ray and thermal neutron treatment. 5. Abnormal mitosis of somatic cell, cell with two nucleoli, cell with two nuclei and chromosome clump in mitosis of somatic cell were observed in Robinia pseudoacacia irradiated with thermal neutron. 6. Resistanty against pawdery mildew was decreased in Robinia pseudoacacia radiated with X-ray and thermal neutron. 7. Length of stomata did not show any difference however number of stomata per unit area decreased in Robinia pseudoacacia radiated with thermal neutron. The leaves of Robinia pseudoacacia radiated with thermal neutron were thicker than those of non-treated one, but width of palisade tissue was decreased. The most sensitive one among those species to the thermal neutron treatment was Larix leptolepis, followed by Pinus densiflora, Robinia pseudoacacia, Pinus thunbergii and Pinus rigida in the order. In X-ray treatment, the most sensitive one was Larix leptolepis, followed by Pinus densiflora, Pinus thunbergii, Pinus rigida and Robinia pseudoacacia in the order. Morphological, cytological variation of the radiated Robinia pseudoacacia seemed to indicate some possibility to be used for tree improvement.

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