• 한국정밀공학회지
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• 제16권11호
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• pp.191-196
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• 1999

This stability of a plate structure is very crucial problem which results in wrinkle and bucking. In this study, the effect of the pattern of spot-welding points of the two rectangular plates on the compressive buckling load is studied with respect to the thickness, aspect ratio of plates, number of welding spots. buckling coefficient of the plate not welded was compared with that of two plates with various thickness to extract the effect of thickness. The effect of number of welding spots are studied in tow directions, longitudinal and transverse directions. The conclusions obtained were that the reinforcement effect was maximized when the aspect ratio was close to 1.25 and that the effect of number of welding spots in transverse direction was large than that in longitudinal direction.

• 한국초지조사료학회지
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• 제10권3호
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• pp.147-151
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• 1990

The purpose of this study is to clarify the relation of survival rates of seedling plants under grown orchardgrass populations and control by the difference cutting frequency. The orchardgrass populations investigated in this study have passed for 10 years after establishment. Cutting frequencies were 3, 4 and 5 times in a year. This experiment was conducted from April to October, 1989 at the experimental field of Institute of Agricultural Development, Yonsei University. The results are summarized as follows: 1. The survival rates of seedling plants decreased by the days after sowing. The final survival rates of seedling plants were 7.8%, 38.9% and 33.8% in 3, 4 and 5 cuttings, respectively. 2. Relative light intensity indicated highly possitive correlation with relative survival rates of seedling plants and negative correlation with plant length of orchardgrass populations in 4 and 5 cuttings. 3. The frequency distribution of survival seedling plants at 177 days after sowing were showed high values in seeding spots near the adjacent plants of 3 cutings, but in the middle of seeding spots between adjacent plants of 4 .and 5 cuttings, respectively. 4. The plant length of seedling became longer towards the seeding spots near the adjacent plants but the number of tillers increased slightly up to the middle of seeding spots between adjacent plants in all cutting frequencies.

• 한국정보과학회논문지:소프트웨어및응용
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• 제32권7호
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• pp.592-600
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• 2005

프레임웍의 기능성을 철저하게 테스팅하기 위해서는 객체 지향 프레임웍의 재사용 시에 확장되는 가변 부위(hot spots)에 대한 체계적인 테스트 패턴 추출이 필수적이다. 본 논문에서는 프레임웍 가변부위의 설계 패턴을 분석하여 가변부위의 상호작용(interaction) 패턴으로부터 테스트 패턴을 추출하는 방법을 제안한다. 프레임웍 가변 부위의 설계 패턴에서 나타날 수 있는 객체들의 상호 작용은 상태도(statechart)로 표현되며, 표현된 상태도는 테스트 패턴 및 테스트 케이스를 생성하는데 사용된다. 생성된 테스트 패턴은 프레임웍을 확장하여 만들어진 어플리케이션들에 반복 적용되어 사용될 수 있다.

• Journal of Information Processing Systems
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• 제16권4호
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• pp.750-759
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• 2020

The wide application of various integrated location-based services (LBS social) and tourism application (app) has generated a large amount of trajectory space data. The trajectory data are used to identify popular tourist attractions with high density of tourists, and they are of great significance to smart service and emergency management of scenic spots. A hot spot analysis method is proposed, based on spatial clustering of trajectory stop points. The DBSCAN algorithm is studied with fast clustering speed, noise processing and clustering of arbitrary shapes in space. The shortage of parameters is manually selected, and an improved method is proposed to adaptively determine parameters based on statistical distribution characteristics of data. DBSCAN clustering analysis and contrast experiments are carried out for three different datasets of artificial synthetic two-dimensional dataset, four-dimensional Iris real dataset and scenic track retention point. The experiment results show that the method can automatically generate reasonable clustering division, and it is superior to traditional algorithms such as DBSCAN and k-means. Finally, based on the spatial clustering results of the trajectory stay points, the Getis-Ord Gi* hotspot analysis and mapping are conducted in ArcGIS software. The hot spots of different tourist attractions are classified according to the analysis results, and the distribution of popular scenic spots is determined with the actual heat of the scenic spots.

• 한국인터넷방송통신학회논문지
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• 제20권6호
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• pp.145-150
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• 2020

정부의 포용적 복지국가 실현 정책에 따라 복지관련 서비스가 다양해지고 복지 급여의 수급 대상자의 범위가 확대됨에 따라 사각지대 관리의 중요성이 높아지고 있다. 이에 따라 사회안전망의 확충이 주요 관심사로 제시되고 있으며 복지위기의 사각지대 관리를 위하여 저소득층 소득과 자립을 지원하고 빈곤·위기 가구를 적극적으로 발굴 및 지원하는 내용의 사회안전망 관리가 필요하다. 본 논문에서는 복지사각지대 관리를 위하여 사각지대 관련 다양한 문제점을 검토하고 사각지대의 범위를 고려하여 블록체인 기반 분산 ID의 적용가능성과 분산 ID(Decentralized Identifier) 기술의 단계별 적용 가능성을 제안하였다.

• 한국정보과학회논문지:소프트웨어및응용
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• 제36권8호
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• pp.653-663
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• 2009

본 논문에서는 세 대의 카메라를 이용하여 사각지대를 효과적으로 제거하기 위한 파노라믹 비전 시스템(panoramic vision system)을 제안한다. 차량의 후방에는 광각 카메라를 사용하여 후방 거울(rear-view mirror)로부터 보이지 않는 사각지대를 제거하며, 나머지 두 대의 카메라는 측면 거울(side-view mirror)의 아래에 설치하여 측면 거울로 보지 못하는 사각지대를 제거한다. 좀 더 효과적인 사각지대 제거를 위해서 세 대의 카메라에서 얻어지는 영상을 정합하는 방법을 제공한다. 먼저, 후방 카메라에서 얻은 영상을 보정하고, 보정된 영상에서 focus-of-contraction(FOC)을 구한다. 그 뒤에 측방 카메라의 영상과 후방 카메라의 영상 사이의 호모그래피(homography)를 구한다. 그 다음으로 영상을 차도와 배경 영역으로 분리하고, 호모그래피를 사용하여 분리한 차도를 정합하고, 가상의 평면에 배경을 투영하여 최종정합을 완성한다. 마지막으로 정합된 영상을 보다 효과적으로 보여주기 위한 실린더 파노라마 영상, 가상의 Top-view 시스템, 그리고 차량의 다양한 정보와 함께 사각지대 영상을 보여주는 통합 정보 가시화 영상을 생성하여 제공한다.

• 대한수의학회지
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• 제50권4호
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• pp.285-295
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• 2010

Protein expression patterns in Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium) strains with diverse phenotypes, such as phage type, antibiotic resistance pattern and plasmid profiles were examined. For detailed analysis of proteins expressed by different S. Typhimurium strains, protein fractions were divided into detergent-rich phase (DP) and aqueous phase (AP) using triton X-114 detergent. The two phases were subjected to two-dimensional gel electrophoresis (2-DE), followed by protein identification using peptide mass fingerprinting (PMF). In the results, PMF showed that DP fractions consisted mainly of outer membrane proteins, whereas the AP fractions included cytosolic proteins. Comparison of 2-DE profiles of DP did not show any distinct protein spots which could be correlated with phage type, antibiotic resistance pattern or plasmid profile. However, comparisons of 2-DE profiles of the AP revealed differences in the protein spots, which could be correlated with the plasmid profile and phage types. Among these protein spots, flagellin was specific for strains containing a 90 kb plasmid. Compared to DT193 phage type, three protein spots in the range of pI 5.0-5.5 and MW 8-15 kDa of AP 2-DE profiles were absent in the DT104 phage types. Additionally, a protein spot with PI in the range of 4.5-5.0 and molecular weight (MW) between 51-69 kDa was specific for phage type DT104, while a protein spot with pI in the range of 4.0-4.8 and MW between 18-20 kDa was specific for DT193 phage type. These protein spots may be useful for discriminating phage types of S. Typhimurium.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.131-131
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• 2017

Though the Platycodon grandiflorum, has a broad range of pharmacologic properties, but the mechanisms underlying these effects remain unclear. In order to profile proteins from the nodal segment, callus, root and shoot, high throughput proteome approach was executed in the present study. Two-dimensional gels stained with CBB, a total of 84 differential expressed proteins were confirmed out of 839 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 58 differential expressed protein spots (${\geq}2-fold$) were analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. Out of 58 differential expressed protein, 32 protein spots were up-regulated such as ribulose-1,5-bisphosphate carboxylase, endoplasmic oxidoreductin-1, heat stress transcription factor A3, RNA pseudourine synthase 4, cysteine proteinase, GntR family transcriptional regulator, E3 xyloglucan 6-xylosyltransferase, while 26 differential protein spots were down-regulated such as L-ascorbate oxidase precursor, late embryogenesis abundant protein D-34, putative SCO1 protein, oxygen-evolving enhancer protein 3. However, the frequency distribution of identified proteins using iProClass databases, and assignment by function based on gene ontology revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding (17%), transferase activity (14%) and ion binding (12%). Taken together, the protein profile may provide insight clues for better understanding the characteristics of proteins and its metabolic activities in various explants of this essential medicinal plant P. grandiflorum.

• Asian-Australasian Journal of Animal Sciences
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• 제28권6호
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• pp.788-795
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• 2015

Two dimensional-fluorescence difference gel electrophoresis (2D DIGE) is an emerging technique for comparative proteomics, which improves the reproducibility and reliability of differential protein expression analysis between samples. The purpose of this study was to investigate bovine pregnancy-specific proteins in the proteome between bovine pregnant and non-pregnant serum using DIGE technique. Serums of 2 pregnant Holstein dairy cattle at day 21 after artificial insemination and those of 2 non-pregnant were used in this study. The pre-electrophoretic labeling of pregnant and non-pregnant serum proteins were mixed with Cy3 and Cy5 fluorescent dyes, respectively, and an internal standard was labeled with Cy2. Labeled proteins with Cy2, Cy3, and Cy5 were separated together in a single gel, and then were detected by fluorescence image analyzer. The 2D DIGE method using fluorescence CyDye DIGE flour had higher sensitivity than conventional 2D gel electrophoresis, and showed reproducible results. Approximately 1,500 protein spots were detected by 2D DIGE. Several proteins showed a more than 1.5-fold up and down regulation between non-pregnant and pregnant serum proteins. The differentially expressed proteins were identified by MALDI-TOF mass spectrometer. A total 16 protein spots were detected to regulate differentially in the pregnant serum, among which 7 spots were up-regulated proteins such as conglutinin precursor, modified bovine fibrinogen and IgG1, and 6 spots were down-regulated proteins such as hemoglobin, complement component 3, bovine fibrinogen and IgG2a three spots were not identified. The identified proteins demonstrate that early pregnant bovine serum may have several pregnancy-specific proteins, and these could be a valuable information for the development of pregnancy-diagnostic markers in early pregnancy bovine serum.

• 한국작물학회지
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• 제60권1호
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• pp.97-106
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• 2015

Platycodon grandiflorum, commonly known as Doraji in Korea, has a wide range of pharmacologic properties, such as reducing adiposity and hyperlipidemia, and antiatherosclerotic effects. However, the mechanisms underlying these effects remain unclear. In order to profile proteins from the nodal segment, callus, root and shoot, high throughput proteome approach was executed in the present study. Two dimensional gels stained with CBB, a total of 84 differential expressed proteins were confirmed out of 839 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 58 differential expressed protein spots (${\geq}$ 2-fold) were analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. Out of 58 differential expressed protein, 32 protein spots were up-regulated such as ribulose-1,5-bisphosphate carboxylase, endoplasmic oxidoreductin-1, heat stress transcription factor A3, RNA pseudourine synthase 4, cysteine proteinase, GntR family transcriptional regulator, E3 xyloglucan 6-xylosyltransferase, while 26 differential protein spots were down-regulated such as L-ascorbate oxidase precursor, late embryogenesis abundant protein D-34, putative SCO1 protein, oxygen-evolving enhancer protein 3. However, frequency distribution of identified proteins using iProClass databases, and assignment by function based on gene ontology revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding (17%), transferase activity (14%) and ion binding (12%). In that way, the exclusive protein profile may provide insight clues for better understanding the characteristics of proteins and metabolic activity in various explants of the economically important medicinal plant Platycodon grandiflorum.