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Analysis of genetic diversity of cowpea landraces from Korea determined by Simple Sequence Repeats and establishment of a core collection

  • Lee, Jeongran;Baek, Hyung-Jin;Yoon, Mun-Sup;Park, Sang-Koo;Cho, Yang-Hee;Kim, Chang-Yung
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.369-376
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    • 2009
  • Cowpea might have been introduced from China to Korea and cultivated for several hundred years but it has never been a staple food crop in Korea. In this study, genetic diversity of 492 Korean cowpea landrace accessions that have passport information was estimated using six SSR markers. The mean of Weir's gene diversity was 0.665 from all accessions investigated in the study. Cowpea gene diversity of six local provinces in Korea was ranged from 0.370 in accessions of Gangwon to 0.680 in Jeonra provinces. Low gene diversity of the cowpea genepool of Gangwon province was probably derived from relatively few introductions. Especially SSR markers VM36 and VM39 seem to be good markers to distinguish the Gangwon accessions from others by occurring at a specific locus with higher than 78% of allele frequency. Except for the Gangwon province with the low genetic diversity, gene diversity of cowpea accessions from other provinces was ranged from 0.600 to 0.680 indicating no big differences among provinces. Distribution pattern of the allele frequencies was similar among the other provinces. This may reveal that Korean farmers might exchange cowpea seeds easily with even their neighbors with geographical barriers. A core collection, 100 landraces, ca. 20% of base collection, was developed at the 70% of a similarity coefficient level using random sampling approaches after stratification of the entire landrace collection based on the phenetic dendrogram. The variability of SSR in the base and core collections of Korean cowpea landrace was compared by calculating Weir's gene diversity. The mean of Weir's gene diversity of the core was 0.707 while that of the base collection was 0.665. The higher diversity index in the core collection indicates that it maintains the initial variability and well represents the base collection. The core collection included one of determinate accession (IT 216155) and two of no branching type accessions (IT 103959 and IT 161024). The core collection could be used to guide more efficient management and utilization of the entire collection. This core collection should be revised periodically as additional accessions are collected and further characterization is conducted.

An Increment of Crossing Efficiency with Consideration of Pollen Viability Analysis in Rose (장미 교배 효율 증대를 위한 화분 임성 검정)

  • Hwang, Yoon Jung;Song, Chang Min;Kwon, Min Kyung;Kim, Sung Tae;Kim, Won Hee;Han, Youn Yol;Han, Tae Ho;Lim, Ki Byung
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.3
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    • pp.193-200
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    • 2010
  • Current rose cultivars are all composed of heterozygous genome due to long history of out crossing including interspecific hybridization. It has been adapted by artificial selection and crossing by breeders that mainly based on the crossing with fertile pollen derived from inter- or intra-specific hybridization. Pollen viability and germination ability tests provide valuable information for the designing of parentage for more successful breeding efficacy. In this study, we tested the pollen viability and germination ability in seven rose cultivars to find any relationship among several factors including pollen size, ploidy levels, and crossing compatibility. The pollen viability showed wide ranges from 39% 'Pinocchio' as minimum to 82% 'Scarlet Mimi' as maximum, whereas pollen germination rate were from 1% 'Mini Rosa' to 41% 'Scarlet Mimi' as a highest. Pollen size ranged from 41.3 to $45.4{\mu}m$ in large sized pollen and 30.7 to $37.4{\mu}m$ in small sized pollen. The mean diameter of large sized pollen is approximately 10-40% bigger than that of small sized pollen. There are positive relationships among ploidy level, total chromosome length, and pollen size. Crossing list showed that seed setting ratio and seed germination were related to pollen viability, pollen germination, and ploidy level.

Comparison of the Effects of Topical Nasal Application on Allergic Rhinitis between Korean and Western Medicine : A Systematic Review of Randomized Controlled Trials (알레르기성 비염에 대한 한약 및 양약 국소비강외용제의 효과 비교 : 체계적 문헌 고찰)

  • Jo, Hyo-Rim;Oh, Se-Hee;Kim, Seon-Hye;Sung, Won-Suk;Hong, Seung-Ug;Kim, Eun-Jung
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.32 no.4
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    • pp.62-89
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    • 2019
  • Objectives : The purpose of this study is to evaluate the effectiveness and safety of topical nasal application of Herbal medicine compared with Western medicine in the treatment of Allergic Rhinitis(AR). Methods : Electronic databases including Cochrane library, PubMed, EMBASE, CNKI, KMBASE, KISS, NDSL, OASIS, KISS and KJTK(Korean Traditional Knowledge Portal) were searched by the keywords such as 'allergic rhinitis', 'nasal sprays', 'herbal medicine', 'plant extracts', and 'external application'. The quality of each RCTs was assessed by Cochrane Collaboration of 'Risk of bias(RoB) Tool'. Results : 19 RCTs were finally selected from 1419 references screened. 19 RCTs were compared with the effects of topical nasal application of Herbal medicine and Western medicine. Based on the symptom scores from 13 RCTs, topical nasal application of herbal medicine generally has a better effect on relief of AR. The two treatments have similar effects on improving the level of specific factors like IgE, IgG, IL-13, $uLTD_4$ in blood and urine. 8 RCTs showed adverse effects(AEs) in both groups and severe AEs were not reported. Conclusions : This study shows that topical nasal application of herbal medicine can improve symptoms and related factors of allergic rhinitis. Well-designed RCT studies with low risk of bias should be conducted to confirm these findings.

The Study for Antioxidative Effects of Acanthopanax sessiliflorus Extract as a New Cosmetics Ingredients Using Electron Paramagnetic Resonance (전자상자성공명법을 이용한 오가피나무추출물의 항산화효과에 대한 연구)

  • Choi, Shin-Wook;Kim, Chang-Su;Choi, Myoung-Soo;Kim, Bo-Hyeon;Kim, Hack-Soo;Choi, Dae-Sung
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.31 no.1 s.49
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    • pp.73-78
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    • 2005
  • This work was carried out to investigate the antioxidative effects of Acanthopnax sessiliflorus from Jeongseon County for the purpose of development of a novel antioxidant from natural products. The antioxidant activity was determined by using electron paramagnetic resonance (EPR), not measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) which have bun used for antioxidant activity of natural sources. Although DPPH radical scavenging activity assay have been generally used for antioxidant activity, this assay is nut appropriated for determinating which radical is scavenged by extracts from natural products. Using EPR, we determinated whether A. sessiliflorus extracts from Jeongseon County scavenge specific radicals or not. On experiment of scavenging superoxide anion radical, hydroxyl radical, nitrogen dioxide and peroxinitrite. Extracts from A. sessiliflorus showed high antioxidant activities to reactive oxygen and nitrogen species. These result suggest that extracts from A. sessiliflorus act as an antioxidant by scavenging reactive oxygen and nitrogen species and used as new cosmetic ingredients for anti-oxidative stress in skin.

Sensitivity Analysis of the Effect of Soil Ecological Quality Information in Selecting Eco-Friendly Road Route (토양생태 등급 정보가 친환경도로노선 선정에 미치는 영향에 관한 민감도 분석)

  • Ki, Dong-Won;Kang, Ho-Geun;Lee, Sang-Eun;Heo, Joon;Park, Joon-Hong
    • Journal of Soil and Groundwater Environment
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    • v.13 no.3
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    • pp.37-44
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    • 2008
  • Soil ecology has important roles in global ecosystems. However, soil ecological quality information is being ignored when assessing ecological impact of construction actions. And methods for classifying and assessing soil ecological quality have been very little established in comparison to those for animal and plant ecosystems. In this study, it was examined whether soil ecological quality information has influence on determining an eco-friendly route for a road construction project. For this, sensitivity analysis was systematically performed by varying the relative significance (weights) of soil ecological quality information among natural environmental and ecological factors. When the weight of soil ecological quality was greater than just 14%, the soil ecological quality information significantly influenced the determination of the eco-friendly routes for a specific road construction project. This demonstrates that soil ecological quality information has to be considered for more reliable environmental impact assessment, and also supports the validity of use of soil ecological quality information and its mapping technique in planning and siting of eco-friendly construction projects.

Effects of Temperature on the Development and Reproduction of Four Species of Aphids (Hemiptera: Aphididae) Damaging Cereal Crops (식량작물에 피해를 주는 진딧물 4종의 발육과 번식에 미치는 온도의 영향)

  • Ahn, Jeong Joon;Choi, Kyung San;Seo, Bo Yoon;Jung, Jin Kyo
    • Korean journal of applied entomology
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    • v.60 no.4
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    • pp.339-355
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    • 2021
  • Aphids can damage plants directly by absorbing their phloem sap and indirectly by transferring plant viruses and causing sooty mold. We compared the thermal effect on the development, survivorship, and reproduction of four cereal crop-damaging aphid species, Rhopalosiphum padi, Aulacorthum solani, Aphis craccivora, and Acyrthosiphon pisum using a life table analysis method. We investigated the stage-specific development period, survivorship, adult longevity, and fecundity of the above mentioned four aphids at 10, 15, 20, 25, and 30℃, respectively, and analyzed their life table parameters using the age-stage, two-sex life table analysis. A. solani nymphs could not complete their development to adulthood at 30℃. The intrinsic increase rate of R. padi was the highest at all tested temperatures except for that at 15℃ (0.12, 0.34, 0.47, and 0.32 at 10, 20, 25, and 30℃, respectively), and that of A. pisum displayed negative values at 30℃ (-0.04). It is speculated that R. padi would be a dominant species under high temperature conditions and A. solani is a highly adaptive species at low temperatures through the comparative analysis of the life table parameters of four aphid species damaging to cereal crops.

Techno-Economic Analysis of Reversible Solid Oxide Fuel Cell System Couple with Waste Steam (폐스팀을 이용한 가역 고체산화물 연료전지의 기술적 경제적 해석)

  • GIAP, VAN-TIEN;LEE, YOUNG DUK;KIM, YOUNG SANG;AHN, KOOK YOUNG
    • Journal of Hydrogen and New Energy
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    • v.30 no.1
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    • pp.21-28
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    • 2019
  • Reversible solid oxide fuel cell (ReSOC) system was integrated with waste steam for electrical energy storage in distributed energy storage application. Waste steam was utilized as external heat in SOEC mode for higher hydrogen production efficiency. Three system configurations were analyzed to evaluate techno-economic performance. The first system is a simple configuration to minimize the cost of balance of plant. The second system is the more complicated configuration with heat recovery steam generator (HRSG). The third system is featured with HRSG and fuel recirculation by blower. Lumped models were used for system performance analyses. The ReSOC stack was characterized by applying area specific resistance value at fixed operating pressure and temperature. In economical assessment, the levelized costs of energy storage (LCOS) were calculated for three system configurations based on capital investment. The system lifetime was assumed 20 years with ReSOC stack replaced every 5 years, inflation rate of 2%, and capacity factor of 80%. The results showed that the exergy round-trip efficiency of system 1, 2, 3 were 47.9%, 48.8%, and 52.8% respectively. The high round-trip efficiency of third system compared to others is attributed to the remarkable reduction in steam requirement and hydrogen compression power owning to fuel recirculation. The result from economic calculation showed that the LCOS values of system 1, 2, 3 were 3.46 ¢/kWh, 3.43 ¢/kWh, and 3.14 ¢/kWh, respectively. Even though the systems 2 and 3 have expensive HRSG, they showed higher round-trip efficiencies and significant reduction in boiler and hydrogen compressor cost.

Process Risk Assessment for a Batch Condensation Reaction of Polyester Resin using K-PSR Technique (K-PSR 기법을 활용한 회분식 폴리에스터 축합반응에서의 공정 위험성 평가 연구)

  • Park, Kyung-Min;Lee, Dong-Kyu;Lee, Haakil;Lee, Joon-Man;Ahn, Won-Sool
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.3
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    • pp.35-42
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    • 2019
  • Risk assessment and analysis for a medium-to-small sized chemical plant that manufactures a polyester resin by the process of batch-type condensation reaction was conducted using K-PSR technique which is one of the risk assessment methods used to implement the Process Safety Management System (PSM). K-PSR is a risk assessment technique developed by KOSHA to compensate for difficulties caused by the lack of infrastructure of medium-to-small sized chemical plants in the re-evaluation. To apply the K-PSR technique, the entire process of a selected chemical palnt was classified in two review sections, i.e., the condensation reaction process and the dilution/filtration process, and the potential risks of the process about these review sections were identified and classified based on the four guide-words (release, fire.explosion, process trouble, and injury). As the results of the research, refer to recommend of risk rating has been confirmed that non-destructive testing of old facilities and the preparation of LOTO procedures for the electrical equipments are necessary as specific measures to prevent the risk of release and fire.explosion. It was also shown that pressure gauges and thermometers should be installed on the hot-oil supply piping to minimize the process trouble, and exhausting hood should be installed to prevent potential injury.

Introduction to Current Status and Researches for Rock Engineering of Finnish Geological Disposal of Spent Fuel (핀란드의 사용후핵연료 지층처분 현황 및 암반공학 관련 연구소개)

  • Hong, Suyeon;Kwon, Saeha;Min, Ki-Bok;Park, Eui-Seob
    • Tunnel and Underground Space
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    • v.29 no.4
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    • pp.215-229
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    • 2019
  • This technical note describes the current status of Finnish radioactive waste disposal project which started to construct the repository for spent nuclear waste for the first time in the world. Finland started operating nuclear power plant in 1977 and is currently operating four nuclear power plants. After detailed site surveys started in 1993, Olkiluoto was finally selected by the parliament of Finland as the site for geological disposal in 2001 followed by a construction license in 2015. If the operating license is approved by the government in the 2020s, it would be the world's first case of geological disposal. In ONKALO, a site-specific underground research facility at the site of Olkiluoto, various studies were conducted to verify the safety of the repository. Finland uses the KBS-3 disposal concept, and Korea considers a similar disposal concept because of similar rock formations. The entire process in Finland including the operation status of intermediate and low-level waste disposal, site investigation and selection stages, and the latest rock mechanics and hydrogeological studies in ONKALO are presented. Suggestions for the radioactive waste disposal in Korea is given based on the Finnish case.

Effects of Overexpression of Brassica rapa GROWTH-REGULATING FACTOR Genes on B. napus Organ Size (배추 GROWTH-REGULATING FACTOR 유전자 발현이 유채 기관크기에 미치는 영향)

  • Hong, Joon Ki;Suh, Eun Jung;Lee, Seung-Bum;Yoon, Hye-Jin;Lee, Yeon-Hee
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.378-386
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    • 2018
  • GROWTH-REGULATING FACTOR (GRF) genes encode plant-specific transcription factors and play critical roles in regulating the growth and development of lateral organs. In order to explore the agricultural potential of Brassica rapa GRF genes (BrGRFs), we constructed two BrGRF-overexpressing B. napus plants (BrGRF3-1OX and -9OX). BrGRF3-1OX and -9OX developed larger cotyledons, leaves, and seeds than the wild type. The increased organs' sizes were due to increases in cell number, but not due to cell size alterations. RT-PCR analysis revealed that BrGRFs regulated the expression of a wide range of genes that are involved in gibberellin-, auxin-, cell division-related growth processes. Taken together, our data indicate that BrGRFs act as positive regulators of B. napus growth, thus raising the possibility that they may serve as a useful genetic source for crop improvement with respect to organ size and seed production.