Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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The Study for Antioxidative Effects of Acanthopanax sessiliflorus Extract as a New Cosmetics Ingredients Using Electron Paramagnetic Resonance

전자상자성공명법을 이용한 오가피나무추출물의 항산화효과에 대한 연구

  • Published : 2005.03.01
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Abstract

This work was carried out to investigate the antioxidative effects of Acanthopnax sessiliflorus from Jeongseon County for the purpose of development of a novel antioxidant from natural products. The antioxidant activity was determined by using electron paramagnetic resonance (EPR), not measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) which have bun used for antioxidant activity of natural sources. Although DPPH radical scavenging activity assay have been generally used for antioxidant activity, this assay is nut appropriated for determinating which radical is scavenged by extracts from natural products. Using EPR, we determinated whether A. sessiliflorus extracts from Jeongseon County scavenge specific radicals or not. On experiment of scavenging superoxide anion radical, hydroxyl radical, nitrogen dioxide and peroxinitrite. Extracts from A. sessiliflorus showed high antioxidant activities to reactive oxygen and nitrogen species. These result suggest that extracts from A. sessiliflorus act as an antioxidant by scavenging reactive oxygen and nitrogen species and used as new cosmetic ingredients for anti-oxidative stress in skin.

본 연구는 천연물로부터 기능성화장품의 소재로 이용할 수 있는 새로운 항산화제를 개발하기 위하여 정선산 오가피나무 추출물의 항산화 효과를 측정하였다. 통상적 항산화력 평가기법인 DPPH 라디칼 소거능 평가 방법은 천연물의 항산화능을 평가하기에 가장 쉬운 방법중에 하나이나 생체내에서 발생되는 특정 라디칼에 대한 항산화능을 평가하기에는 부적합하여 본 연구진은 전사상자성공명법이라는 방법을 이용하여 특정 라디칼에 대한 오가피나무 추출물의 항산화능을 평가하였다. 전자상자성공명법을 이용한 항산화능 평가 결과 오가피나무의 추출물은 유해산소종 및 유해질소종에 대한 매우 우수한 항산화능을 가지고 있음이 밝혀졌으며, 이리한 결과로 보아 오가피나무 추출물은 새로운 항산화제 소재 및 피부내의 산화적 스트레스 방지목적의 기능성 화장품소재로서의 개발가능성이 매우 높음을 알 수 있었다.

Keywords

References

  1. P. Ody, Herbal insights - A close look at active constituents of medical herbs, SOFE Journal, 121, 8 (1995)
  2. E. A. Lissi, M. Salim-Hanna, C. Psacual, and M. D. Castillo, Evaluation of total antioxidant potencial and total reactivity from lurninol-enhanced chemiluminescence measurements, Free Radic. Biol. Med, 18(2), 153 (1995) https://doi.org/10.1016/0891-5849(94)00117-3
  3. A. Ghiselli, M. Serafini, G. Maiani, E. Azzini, and A. Ferro-Luzzi, A fluorescence-based methods for measuring total plasma antioxidant capability, Free Rad. Biol. Med, 18(1), 29 (1995) https://doi.org/10.1016/0891-5849(94)00102-P
  4. M. M. Corl, Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene, J. Am Oil Chem Soc, 52(1), 59 (1975) https://doi.org/10.1007/BF02901825
  5. Y. H. Choi, M, J. Kim, H. S. Lee, B. S. Yun, C. Hu, and S. S. Kwak, Antioxidative compounds in aerial parts of Potentilla fragarioides, Kor. J. Pharmacon, 31(1), 79 (1998)
  6. 김창민, 신민교, 안덕균, 이경순, 완역중약대사전, 3907, 도서출판 정담, 서울 (1998)
  7. S. K. Kim, Y. G. Kim, M. K. Lee, J. S. Ham, J. H. Lee, and H. Y. Lee, Comparison of biological activity according to extracting solvents of four Acanthopananx root bark, Korean J. Medical Corp. Sci, 8(1), 21 (2000)
  8. J. K. Ahn, W. Y. Lee, S. J. Oh, Y. H. Park, S. D. Hur, and M. S. Choi, The contents of chlorogeic acid and eluthersoside E in Eleutherococcus senticocus (Ruper. et Maxim) Harms, J. Korean For. Soc, 89(2), 211 (2000)
  9. M. Miyakoshi, Y. Ida, S. Isoda, and J. Shoji, Alpha-hydroxyoleanane-type triterpene glycosyl esters from leaves of Aamihopanax spinosus, Phytochemistry, 34(6), 1599 (1993) https://doi.org/10.1016/S0031-9422(00)90853-7
  10. H. Sawada, M. Miyakoshi, S. Isoda, Y. Ida, and J. Shoji, Saponins from leaves of Acanthopanax sie-boldianus, Phytochernisty, 34(4), 1117 (1993)
  11. Y. Q. Zhao, S. S. Yang, J. H. Liu, and G. R. Zhao, Chemical constitutents of Acanihopanax senticocus (Rupt. et Maxim) Harms, Zhongguo Zhong Yao Za Zhi, 18(7), 428 (1993)
  12. J. Z. Wang, H. Tsumura, N. Ma, K. Shimura, and H. Ito, Biochemical and morphological alterations of macrophages and spleen cells produced by antitumor polysaccharide from Acanihopanax obovatus roots, Planta Med., 59(1), 54 (1993)
  13. J. H. lung, S. W. Choi, and S. Han, Screening for antioxidative effects of cultivated crop from jeongsean county using electron paramagnetic resonance, J. Sanhakyeon Reg. Consortium Center, Kangwon Nat'l Univ., 7, 23 (2003)