Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
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Effects of Overexpression of Brassica rapa GROWTH-REGULATING FACTOR Genes on B. napus Organ Size

배추 GROWTH-REGULATING FACTOR 유전자 발현이 유채 기관크기에 미치는 영향

  • Hong, Joon Ki (Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Suh, Eun Jung (Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Seung-Bum (Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Yoon, Hye-Jin (Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Yeon-Hee (Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration)
  • 홍준기 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 서은정 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 이승범 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 윤혜진 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 이연희 (농촌진흥청 국립농업과학원 농업생명자원부)
  • Received : 2018.07.31
  • Accepted : 2018.08.24
  • Published : 2018.12.01
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Abstract

GROWTH-REGULATING FACTOR (GRF) genes encode plant-specific transcription factors and play critical roles in regulating the growth and development of lateral organs. In order to explore the agricultural potential of Brassica rapa GRF genes (BrGRFs), we constructed two BrGRF-overexpressing B. napus plants (BrGRF3-1OX and -9OX). BrGRF3-1OX and -9OX developed larger cotyledons, leaves, and seeds than the wild type. The increased organs' sizes were due to increases in cell number, but not due to cell size alterations. RT-PCR analysis revealed that BrGRFs regulated the expression of a wide range of genes that are involved in gibberellin-, auxin-, cell division-related growth processes. Taken together, our data indicate that BrGRFs act as positive regulators of B. napus growth, thus raising the possibility that they may serve as a useful genetic source for crop improvement with respect to organ size and seed production.

GROWTH-REGULATING FACTOR (GRF) 유전자는 QLQ와 WRC 도메인을 갖고 있는 식물 특이적 전사조절인자로 식물기관들의 생장과 발달에 중요한 역할을 하고 있다. 배추로부터 분리된 BrGRF3-1과 BrGRF9 유전자를 각각 35S 프로모터에 결합시켜 두 종류의 식물 형질전환 벡터를 제작한 후 아그로박테리움을 이용하여 유채에 형질전환하였다. 선발된 형질전환체의 특성을 분석한 결과 세포 크기 보다는 세포 수 증가에 의해 자엽, 잎, 종자의 크기가 커지는 것으로 나타났다. RT-PCR로 유전자 발현을 분석한 결과 BrGRF3-1과 BrGRF9 유전자는 기관 생장 발달에 관여하는 지베렐린(GA), 옥신(auxin), 세포분열 관련 유전자들의 발현을 조절함으로써 유채 기관의 생장과 발달에 중요한 역할을 하는 것으로 추측된다. 따라서 본 연구결과로 BrGRF 유전자는 생명공학기술을 활용하여 작물의 농업적 형질을 개선하기 위한 유전자원으로 이용될 수 있을 것으로 사료된다.

Keywords

Acknowledgement

Supported by : 국립농업과학원

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