• 식물병연구
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• 제19권4호
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• pp.319-325
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• 2013

Soybean yellow mottle mosaic virus(SYMMV)와 Soybean yellow common mosaic virus(SYCMV) 병 발생은 최근에 우리나라에 보고되었지만 두 바이러스 병의 전국적인 분포에 대한 조사가 이루어지지 않아 본 연구팀은 2012년에 영세 농가를 중심으로 전국적인 조사를 실시하였다. 전국에 걸쳐 채집한 682점의 시료를 SYMMV, SYCMV, 그리고 Soybean mosaic virus(SMV) 특이 프라이머를 이용하여 진단한 결과, SMV 102점, SYMMV 116점, SYCMV는 17점이 PCR 양성으로 나타났다. SYMMV와 SYCMV의 복합감염은 검출되지 않았으나, SMV와 SYMMV 혹은 SMV와 SYCMV 복합감염은 각각 1건과 5건이 확인되었다. 처음으로 실시한 영세농가의 콩 바이러스 병 조사를 근거로 추정할 때, 바이러스의 발생은 자가 채종으로 인한 종자전염으로부터 발생되었을 것으로 추정된다. 따라서 바이러스 병의 예방을 위해서는 감염종자 관리 및 무병종자 보급이 무엇보다 필요함을 알 수 있다.

• 식물병연구
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• 제13권1호
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• pp.30-33
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• 2007

석량풋콩 품종의 콩밭에서 모자이크 증상이 약하게 나타난 186개의 잎을 채집하여, Cowpea mosaic virus(CPMV)에 대한 ELISA 검정을 하였다. 그 결과 93개의 시료가 양성반응을 보였으며, 이 가운데 55개의 시료는 매우 높은 바이러스 밀도를 나타내고 있었다. 이러한 결과는 석량풋콩 품종의 동부모자이크병은 ELISA 검정, 바이러스 순화, 전자현미경검경 결과와 바이러스 표피 단백질분석 결과 Cowpea mosaic virus에 의한 병해로 확인되었다.

• Journal of Applied Biological Chemistry
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• 제63권3호
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• pp.189-195
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• 2020

The soybean (Glycine max L.), also known as the soya bean, is an economically important legume species. Pathogens are always major threats for soybean cultivation. Several pathogens negatively affect soybean production. The soybean is also known as a susceptible host to many viruses. Recently, we carried out systematic analyses to identify viruses infecting soybeans using soybean transcriptome data. Our screening results showed that only few soybean transcriptomes contained virus-associated sequences. In this study, we further carried out bioinformatics analyses using a soybean flower bud transcriptome for virus identification, genome assembly, and single nucleotide variations (SNVs). We assembled the genome of Soybean yellow common mosaic virus (SYCMV) isolate China and revealed two SNVs. Phylogenetic analyses using three viral proteins suggested that SYCMV isolate China is closely related to SYCMV isolates from South Korea. Furthermore, we found that replication and mutation of SYCMV is relatively low, which might be associated with flower bud tissue. The most interesting finding was that SYCMV was not detected in the cytoplasmic male sterility (CMS) line derived from the non-CMS line that was severely infected by SYCMV. In summary, in silico analyses identified SYCMV from the soybean flower bud transcriptome, and a nearly complete genome of SYCMV was successfully assembled. Our results suggest that the low level of virus replication and mutation for SYCMV might be associated with plant tissues. Moreover, we provide the first evidence that male sterility might be used to eliminate viruses in crop plants.

• The Plant Pathology Journal
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• 제33권4호
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• pp.424-428
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• 2017

Tomato yellow leaf curl virus (TYLCV) infection of the common bean (Phaseolus vulgaris) has been reported, but soybean (Glycine max) has not previously been identified as a TYLCV host. Five cultivars of white soybean were agro-inoculated using an infectious TYLCV clone. At 30 days post-inoculation, they showed infection rates of 25% to 100%. Typical TYLCV symptoms were not observed in any inoculated plants. To examine whether TYLCV was transmitted in soybean seeds, DNA was isolated from bundles of five randomly selected seeds from TYLCV-inoculated soybean plants and amplified with a TYLCV-specific primer set. With the exception of one bundle, all bundles of seeds were verified to be TYLCV-infected. Virus dissemination was also confirmed in three of the 14 bunches. Viral replication was also identified in seeds and seedlings. This is the first report demonstrating that soybean is a TYLCV host, and that TYLCV is a seed-transmissible virus in white soybean.

• 식물병과 농업
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• 제1권2호
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• pp.22-25
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• 1995

The soybean necrotic disease has been shown to be caused by a virulent strain or strains of soybean mosaic virus (SMV) in soybean cultivar Kwnaggyo. However, the disease was found in soybean cultivar Hwanggeum which was released as a leading and mosaic resistant soybean cultivar in Korea. The strain SMV-G5H appeared to an isolate showing similar characteristics with the strain SMV-G7, although there were some variations in reactions of soybean differentials used.

• The Plant Pathology Journal
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• 제19권3호
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• pp.166-170
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• 2003

Ninety samples showing mosaic symptoms on soybean (Glycine max) cv. Sukryangputkong were collected from the Cheongsongkun area, Kyungbuk province in Korea. Initially, DAS-ELISA was conducted far detection of Soybean mosaic virus (SMV). Negative samples were chosen at random and mechanically inoculated on soybean cv. Buffalo, which reported not to produce mosaic symptoms when mechanically inoculated with SMV. An isolate of SMV, designated as B-1, from Buffalo showing mosaic and mottle symptoms was used for identification and biological characterization of the causal vim. The purified B-1 isolate had spherical particles of approximately 24nm. It positively reacted with the antiserum against Cowpea mosaic virus (CPMV) but not with Cucumber mosaic virus (CMV) and SMV antisera. CPMV was newly isolated from soybean and had been characterized by host range and by serological and electron microscopic methods. Results of this study suggest that CPMV is the possible cause of mosaic disease in vegetable soybean and that based on sympto-matology, a difference between the typical mosaic and rugose symptoms caused by SMV and CPMV was observed. This is first report of CPMV from soybean in Korea.

• The Plant Pathology Journal
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• 제27권4호
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• pp.315-323
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• 2011

Soybean plants infected with Bean pod mottle virus (BPMV) develop acute symptoms that usually decrease in severity over time. In other plant-virus interactions, this type of symptom recovery has been associated with degradation of viral RNAs by RNA silencing, which is accompanied by the accumulation of virus-derived small interfering RNAs (siRNAs). In this study, changes in the accumulation of BPMV siRNAs were investigated in soybean plants infected with BPMV alone, or infected with both BPMV and Soybean mosaic virus (SMV) and in transgenic soybean plants expressing SMV helper component-protease (HC-Pro). In many potyviruses, HC-Pro is a potent suppressor of RNA silencing. In plants infected with BPMV alone, accumulation of siRNAs was positively correlated with symptom severity and accumulation of BPMV genomic RNAs. Plants infected with both BPMV and SMV and BPMV-infected transgenic soybean plants expressing SMV HC-Pro exhibited severe symptoms characteristic of BPMVSMV synergism, and showed enhanced accumulation of BPMV RNAs and siRNAs compared to plants infected with BPMV alone and nontransgenic plants. Likewise, SMV HC-Pro enhanced the accumulation of siRNAs produced from a silenced green fluorescent protein gene in transient expression assays, while the P19 silencing suppressor of Tomato bushy stunt virus did not. Consistent with the modes of action of HC-Pro in other systems, which have shown that HC-Pro suppresses RNA silencing by preventing the unwinding of duplex siRNAs and inhibiting siRNA methylation, these studies showed that SMV HC-Pro interfered with the activities of RNA-induced silencing complexes, but not the activities of Dicer-like enzymes in antiviral defenses.

• 한국응용곤충학회지
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• 제15권2호
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• pp.61-68
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• 1976

광교, 동북태, 강림, 육우3호, 은대두등과 같은 대리장려품종이 바이러스에 의하여 심하게 이병되었다. 이 병은 주로 모자익병의 발생이 많은 강원, 경기지방에서 발병이 심하였으나 모자익병이 심하지 않은 전남 등 남부지방에서도 발병되고 있다. 병징으로 보아 tobacco ringspot virus에 의한 대두의 피해와 유사한 것으로 보였으나 지표식물검정과 혈청검정에 의하여 조사한 결과 모두 부정적이었으며 대두품종에 따른 이병정도의 상이, 품종과 접종원에 의한 병징의 변이가 많았다. 이병주에서 분리되는 병징형은 Mottling과 necrosis였으며 지금까지의 연구결과 이 대두병해는 모자익바이러스(SMV)의 계통 내지는 tobacco ringspot virus 이외의 두류바이러스의 복각감염에 의한 것으로 생각할 수 있으나 SMV의 계통에 의한 피해일 가능성이 더욱 유력시되고 있다.

• Journal of Plant Biology
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• 제16권3_4호
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• pp.35-39
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• 1973

This investigation was conducted to study the effect of soybean mosaic virus (SMV) on various parameters of nodule formation at different stage of soybean plants. Differences in nodule formation were marked between soybean varieties tested, but nodules were small within soybean varieties infected with SMV. SMV-infection on soybeans were greatly reduced the number, size and weight of nodules, and the earlier the infection of SMV, the greater the reduction of nodules. Maximum reduction(83%) of nodules observed when "Kumkang-Daerip" soybeans were inoculated 2 weeks after seeding, but none occurred 8 weeks or later. Prominent decreases in number of nodules often resulted in an increase in nodu'e sizes in SMV-infected soybean plants.an plants.

• The Plant Pathology Journal
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• 제33권5호
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• pp.478-487
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• 2017

Soybean is the most important legume crop in the world. Several diseases in soybean lead to serious yield losses in major soybean-producing countries. Moreover, soybean can be infected by diverse viruses. Recently, we carried out a large-scale screening to identify viruses infecting soybean using available soybean transcriptome data. Of the screened transcriptomes, a soybean transcriptome for soybean seed development analysis contains several virus-associated sequences. In this study, we identified five viruses, including soybean mosaic virus (SMV), infecting soybean by de novo transcriptome assembly followed by blast search. We assembled a nearly complete consensus genome sequence of SMV China using transcriptome data. Based on phylogenetic analysis, the consensus genome sequence of SMV China was closely related to SMV isolates from South Korea. We examined single nucleotide variations (SNVs) for SMVs in the soybean seed transcriptome revealing 780 SNVs, which were evenly distributed on the SMV genome. Four SNVs, C-U, U-C, A-G, and G-A, were frequently identified. This result demonstrated the quasispecies variation of the SMV genome. Taken together, this study carried out bioinformatics analyses to identify viruses using soybean transcriptome data. In addition, we demonstrated the application of soybean transcriptome data for virus genome assembly and SNV analysis.