Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Occurrence of Three Major Soybean Viruses, Soybean mosaic virus, Soybean yellow mottle mosaic virus and Soybean yellow common mosaic virus Revealed by a Nationwide Survey of Subsistence Farming Soybean Fields

영세농가 콩 재배지의 Soybean mosaic virus, Soybean yellow mottle mosaic virus 및 Soybean yellow common mosaic virus 병 발생 조사

  • Cho, Seunghee (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Jungkyu (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Li, Meijia (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Seo, Eunyoung (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Lim, Seungmo (Greenbio Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Hong, Seok Myeong (GenoRevo Korea Co., Ltd.) ;
  • Moon, Jae Sun (Greenbio Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Hammond, John (United States Department of Agriculture-Agrocultural Research Service, Floral and Nursery Plants Research Unit.) ;
  • Lim, Hyoun-Sub (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • 조승희 (충남대학교 응용생물학과) ;
  • 김정규 (충남대학교 응용생물학과) ;
  • 이미가 (충남대학교 응용생물학과) ;
  • 서은영 (충남대학교 응용생물학과) ;
  • 임승모 (한국생명공학연구원 그린바이오연구센터) ;
  • 홍석명 ((주)제노레버코리아) ;
  • 문제선 (한국생명공학연구원 그린바이오연구센터) ;
  • ;
  • 임현섭 (충남대학교 응용생물학과)
  • Received : 2013.10.07
  • Accepted : 2013.11.04
  • Published : 2013.12.31
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Abstract

Soybean yellow mottle mosaic virus (SYMMV) and Soybean yellow common mosaic virus (SYCMV) were recently isolated in Korea, and it has not been reported how two viruses were dispersed in Korea. In 2012, we performed nationwide survey in subsistence soybean farming. Suspicious virus-infected infected leave were collected from the field and a total of 682 soybean tissue samples were assayed by RT-PCR using triplex primers detecting SYMMV, SYCMV, and Soybean mosaic virus (SMV). On hundred two samples showed SMV positive, and SYMMV and SYCMV were detected in 116 and 17 tissue samples, respectively. No sample showed double infection of SYMMV and SYCMV, but there were double infection tissues indicating two viruses positive of SMV plus SYMMV (5 tissue samples) and SMV plus SYCMV (1 tissue sample). Through this first subsistence soybean farming field survey, we assumed soybean viruses were originated from home seed production managed by farmer. Thus, in order to prevent possible seed transmission and further damage caused by virus transmission, virus-free commercial soybean seeds are recommended to be planted.

Soybean yellow mottle mosaic virus(SYMMV)와 Soybean yellow common mosaic virus(SYCMV) 병 발생은 최근에 우리나라에 보고되었지만 두 바이러스 병의 전국적인 분포에 대한 조사가 이루어지지 않아 본 연구팀은 2012년에 영세 농가를 중심으로 전국적인 조사를 실시하였다. 전국에 걸쳐 채집한 682점의 시료를 SYMMV, SYCMV, 그리고 Soybean mosaic virus(SMV) 특이 프라이머를 이용하여 진단한 결과, SMV 102점, SYMMV 116점, SYCMV는 17점이 PCR 양성으로 나타났다. SYMMV와 SYCMV의 복합감염은 검출되지 않았으나, SMV와 SYMMV 혹은 SMV와 SYCMV 복합감염은 각각 1건과 5건이 확인되었다. 처음으로 실시한 영세농가의 콩 바이러스 병 조사를 근거로 추정할 때, 바이러스의 발생은 자가 채종으로 인한 종자전염으로부터 발생되었을 것으로 추정된다. 따라서 바이러스 병의 예방을 위해서는 감염종자 관리 및 무병종자 보급이 무엇보다 필요함을 알 수 있다.

Keywords

References

  1. Bae, H. H., Kim, M. S., Sicher, R. C., Bae, H. J. and Bailey, B. A. 2006. Necrosis- and ethylene-inducing peptide from Fusarium oxysporum induces a complex cascade of transcripts associated with signal transduction and cell death in arabidopsis[W]. Plant Physiol. 141: 1056-1067. https://doi.org/10.1104/pp.106.076869
  2. Domier, L. L., Hobbs, H. A., McCoppin, N. K., Bowen, C. R., Steinlage, T. A., Chang, S. Y., Wang, Y. and Hartman G. L. 2011. Multiple loci condition seed transmission of Soybean mosaic virus (SMV) and SMV-induced seed coat mottling in soybean. Phytopathology 101: 750-756. https://doi.org/10.1094/PHYTO-09-10-0239
  3. Dudek, S. G. 2001. Nutrition essentials for nursing practice (4th ed.). Philadelphia: Lippincott.
  4. Evans, B. A., Griffiths, K. and Morton, M. S. 1995. Inhibition of 5alpha-reductase in genital skin fibroblasts and prostate tissue by dietary lignans and isoflavonoids. J. Clin. Endocrinol. Metab. 147: 295-302.
  5. Folkman, J. and Shing, Y. 1992. Angiogenesis. J. Biol. Chem. 267: 10931-10934.
  6. Harper, M. E., Glynne-Jones, E., Goddard, L., Thurston, V. J. and Griffiths, K. 1996. Vascular endothelial growth factor (VEGF) expression in prostatic tumours and its relationship to neuroendocrine cells. Br. J. Cancer. 74: 910-916. https://doi.org/10.1038/bjc.1996.456
  7. Jossey, S., Hobbs, H. A. and Domier, L. L. 2013. Role of Soybean mosaic virus-encoded proteins in seed and aphid transmission in soybean. Phytopathology 103: 941-948. https://doi.org/10.1094/PHYTO-09-12-0248-R
  8. Kitur, B. K., Phillips, S. R., Olson, K. R. and Ebelhar, S. A. 1994. Tillage effects on selected chemical properties of grantsburg silt loam1. Commun. Soil Sci. Plant Anal. 25: 3-4.
  9. Lee, Y. H., Lim, S. T., Yoon, Y. N., Jeon, M. G., Yun, H. T., Ko, J. M., Lee, S. H., Lee, K. W. and Baek, I. Y. 2012. Incidence of soybean viral diseases in Korea. Korea Soybean Digest. 29: 7-15.
  10. Lim, H. S., Ko, T. S., Lambert, K. N., Kim, H. G., Korban, S. S., Hartman, G. L. and Domier, L. L. 2005. Soybean mosaic virus helper component-protease enhances somatic embryo production and stabilizes transgene expression in soybean. Plant Physiol. Biochem. 43: 1014-1021. https://doi.org/10.1016/j.plaphy.2005.08.012
  11. Lim, H. S., Vaira, A. M., Reinsel, M. D., Bae, H. H., Bailey, B. A., Domier, L. L. and Hammond, J. 2010. Pathogenicity of Alternanthera mosaic virus is affected by determinants in RNA-dependent RNA polymerase and by reduced efficacy of silencing suppression in a movement-competent TGB1. J. Gen. Virol. 91: 2102-2115. https://doi.org/10.1099/vir.0.019448-0
  12. Nakajima, A., Usui, M., Huy, T. T. T., Hlaing, N. K. T., Masaki, N., Sata, T. and Abe, K. 2005. Full-length sequence of hepatitis B virus belonging to genotype H identified in a Japanese patient with chronic hepatitis. Jpn. J. Infect. Dis. 58:244-246.
  13. Nam, M., Kim, J. S., Park, S. J., Park, C. Y., Lee, J. S., Choi, H. S., Kim, J. S., Kim, H. G., Lim, S. M., Moon, J. S. and Lee, S. H. 2011. Biological and molecular characterization of Soybean yellow common mosaic virus, a new species in the genus Sobemovirus. Virus Res. 163: 363-367.
  14. Nam, M., Kim, S. M., Domier, L. L., Koh, S., Moon, J. K., Choi, H. S., Kim, H. G., Moon, J. S. and Lee, S. H. 2009. Nucleotide sequence and genomic organization of a newly identified member of the genus Carmovirus, Soybean yellow mottle mosaic virus, from soybean. Arch Virol. 154: 1679-1684. https://doi.org/10.1007/s00705-009-0480-z
  15. Parks, T. D., Howard, E. D., Wolpert, T. J., Arp, D. J. and Dougherty, W. G. 1995. Expression and purification of a recombinant Tobacco etch virus NIa proteinase: Biochemical analyses of the full-length and a naturally occurring truncated proteinase form. Virology 210: 194-201. https://doi.org/10.1006/viro.1995.1331
  16. Rice-Evans, C. A., Miller, N. J., Bolwell, P. G., Bramley, P. M. and Pridham, J. B. 1995. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radical Res. 22: 375-383. https://doi.org/10.3109/10715769509145649
  17. Xu, D. H., Abe, J., Gai, J. Y. and Shimamoto, Y. 2002. Diversity of chloroplast DNA SSRs in wild and cultivated soybeans: evidence for multiple origins of cultivated soybean. Theor. Appl. Genet. 105: 645-653. https://doi.org/10.1007/s00122-002-0972-7