Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Identification of Cherry green ring mottle virus on Sweet Cherry Trees in Korea

국내 양앵두나무에서 발생한 Cherry green ring mottle virus 동정

  • Cho, In-Sook (Horticultural and Herbal Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Choi, Gug-Seoun (Horticultural and Herbal Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Choi, Seung-Kook (Horticultural and Herbal Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 조인숙 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 최국선 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 최승국 (농촌진흥청 국립원예특작과학원 원예특작환경과)
  • Received : 2013.10.11
  • Accepted : 2013.11.05
  • Published : 2013.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

During the 2012 growing season, 154 leaf samples were collected from sweet cherry trees in Hwaseong, Pyeongtaek, Gyeongju, Kimcheon, Daegu, Yeongju and Eumseong and tested for the presence of Cherry green ring mottle virus (CGRMV). PCR products of the expected size (807 bp) were obtained from 6 samples. The PCR products were cloned and sequenced. The nucleotide sequences of the clones showed over 88% identities to published coat protein sequences of CGRMV isolates in the GenBank database. The sequences of CGRMV isolates, CGR-KO 1-6 shared 98.8 to 99.8% nucleotide and 99.6 to 100% amino acid similarities. Phylogenetic analysis indicated that the Korean CGRMV isolates belong to the group II of CGRMV coat protein genes. The CGRMV infected sweet cherry trees were also tested for Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), Cherry necrotic rusty mottle virus (CNRMV), Cherry mottle leaf virus (CMLV), Cherry rasp leaf virus (CRLV), Cherry leafroll virus (CLRV), Cherry virus A (CVA), Little cherry virus 1 (LChV1), Prune dwarf virus (PDV) and Prunus necrotic ringspot virus (PNRSV) by RT-PCR. All of the tested trees were also infected with ACLSV.

2012년 국내 양앵두나무에서 발생하는 CGRMV를 조사하기 위해 화성, 평택, 경주, 김천, 대구, 영주 음성의 양앵두 재배과원 7개 지역에서 잎 시료 154점을 채집하였다. 채집한 시료에 대해 CGRMV 유전자 검정을 수행한 결과 6점의 시료에서 807 bp 크기의 PCR 증폭산물이 검출되었다. 이들 PCR 증폭산물은 클로닝과 유전자 염기서열 분석 결과 GeneBank에 등록된 외국의 CGRMV 분리주들과 88% 이상의 외피단백질 유전자 염기서열 상동성을 보였다. 국내 양앵두나무에서 분리된 분리주들, CGR-KO 1-6 간에는 98.8-99.8%의 염기서열 및 99.6-100%의 아미노산 서열 상동성을 나타내었다. 국내 CGRMV 분리주들은 외피단백질 유전자 계통도 분석에서 기존에 분류된 I, II, III 그룹 중 II 그룹에 속하였다. 또한 CGRMV가 감염된 국내 양앵두나무 이병주들은 ACLSV 등 10종 바이러스에 대해서도 RT-PCR을 수행한 결과 모든 시료에서 ACLSV가 검출되어 CGRMV와 ACLSV가 복합 감염된 것을 확인하였다.

Keywords

References

  1. Cho, I. S. 2011. Development of diagnostic and control techniques for horticultural diseases and insect pests. In: Res. Rept. RDA (NIHHS), pp. 33-34. (In Korean)
  2. Cho, I. S., Choi, G. S., Choi, S. K., Seo, E. Y. and Lim, H. S. 2013. First report of Cherry necrotic rusty mottle virus infecting sweet cherry trees in Korea. Plant Dis. http://dx.doi.org/10.1094/PDIS-07-13-0723-PDN.
  3. Fiore, N. and Zamorano, A. 2013. First report of Cherry green ring mottle virus and Cherry necrotic rusty mottle virus in sweet cherry (Prunus avium) in Chile and South America. Plant Dis. 97: 1122.
  4. Isogai, M., Aoyagi, J., Nakagawa, M., Kubodera, Y., Satoh, K., Katoh, T., Inamori, M., Yamashita, K. and Yoshikawa, N. 2004. Molecular detection of five cherry viruses from sweet cherry trees in Japan. J. Gen. Plant Pathol. 70: 288-291. https://doi.org/10.1007/s10327-004-0129-4
  5. Li, R. and Mock, R. 2005. An improved reverse transcriptionpolymerase chain reaction (RT-PCR) assay for the detection of two cherry flexiviruses in Prunus spp. J. Virol. Meth. 129: 162-169. https://doi.org/10.1016/j.jviromet.2005.05.019
  6. Li, R., Mock, R., Huang, Q., Abad, J., Hartung, J. and Kinarda, G. 2008. A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens. J. Virol. Meth. 154: 48-55. https://doi.org/10.1016/j.jviromet.2008.09.008
  7. Mandic, B., Matic, S., Al Rwahnih, M., Jelkmann, W. and Myrta, A. 2007. Viruses of sweet and sour cherry in Serbia. J. Plant Pathol. 89: 103-108.
  8. Wang, L. P., Hong, N., Wang, G. P., Michelutti, R. and Zhang, B. L. 2009. First report of Cherry green ring mottle virus in plum (Prunus domestica) in North America. Plant Dis. 93: 1073.
  9. Wang, L., Jiang, D., Niu, F., Lu, M., Wang, H. and Li, S. 2013. Complete nucleotide sequences of two isolates of Cherry green ring mottle virus from peach (Prunus persica) in China. Arch. Virol. 158: 707-710. https://doi.org/10.1007/s00705-012-1521-6
  10. Yoon, I. K., Nam, E. Y., Chung, K. H. and Yun, S. K. 2012. Cherry cultivation manual. RDA, pp. 9-10. (In Korean)
  11. Zhang, Y. P., Kirkpatrick, B. C., Smart, C. D. and Uyemoto, J. K. 1998. cDNA cloning and molecular characterization of Cherry green ring mottle virus. J. Gen. Virol. 79: 2275-2281. https://doi.org/10.1099/0022-1317-79-9-2275
  12. Zhang, Y. P., Kirkpatrick, B. C., Terlizzi, B. D. and Uyemoto, J. K. 2000. Comparison of Cherry green ring mottle virus strains using RT-PCR and coat protein sequence phylogeny. J. Plant Pathol. 82: 49-53.
  13. Zhou, J. F., Wang, G. P., Kuang, R. F., Wang, L. P. and Hong, N. 2011. First report of Cherry green ring mottle virus on cherry and peach grown in China. Plant Dis. 95: 1319.

Cited by

  1. Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR vol.30, pp.4, 2014, https://doi.org/10.5423/PPJ.NT.05.2014.0041
  2. in Sweet Cherry in Korea vol.102, pp.5, 2018, https://doi.org/10.1094/PDIS-06-17-0783-PDN