• Journal of Microbiology and Biotechnology
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• v.18 no.7
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• pp.1203-1206
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• 2008

A Gram-positive strain designated as MSL-$14^T$ isolated from a soil sample collected from Bigeum Island, Korea, was subjected to polyphasic taxonomy. The isolate was strictly aerobic. Cells were short rods and motile. Optimum growth temperature and pH was 28$^{\circ}C$ and 7.0, respectively. It was characterized chemotaxonomically as having a cell-wall peptidoglycan type based on LL-2,6-diaminopimelic acid and MK-$8(H_4)$ as the predominant menaquinone. The major fatty acids were iso-$C_{16:0}$, $C_{17:1}$ omega8c, and $C_{18:1}$ omega9c. The G+C content was 67.6 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain MSL-$14^T$ is affiliated to the genus Nocardioides and formed a distinct lineage within the genus. MSL-$14^T$ showed highest sequence similarity to Nocardioides aestuarii JCM $12125^T$, having a similarity of 96.5%. Based on the 16S rRNA gene sequence divergence and phenotypic characteristics, it is proposed that strain MSL-$14^T$ should be classified as representing a novel member of the genus Nocardioides, for which we propose the name Nocardioides tritolerans sp. novo The type strain is strain MSL-$14^T$ (=KCTC $19289^T$=DSM $19320^T$).

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.2
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• pp.117-121
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• 2013

Single or co-inoculation of phosphate solubilizing bacterial and fungal strains (Burkholderia anthina and Aspergillus awamori respectively) was performed separately to assess their synergistic and antagonistic interactions and the potential to be used as bio-inoculants. Co-inoculation was found to release the highest content of soluble phosphorus (1253 ${\mu}g\;ml^{-1}$) into the medium, followed by single inoculation of fungal strain (1214 ${\mu}g\;ml^{-1}$) and bacterial strain (997 ${\mu}g\;ml^{-1}$). However, there was no significant difference between single inoculation of fungal strain and co-inoculation of fungal and bacterial strain in terms of the phosphorous release. The highest pH reduction, organic acid production and glucose consumption were observed in the sole A. awamori inoculated culture medium. According to the plant growth promotion bioassays, co-inoculation of the microbial strains resulted in 21% and 43% higher shoot and root growth of the mung bean seedlings respectively as compared to the respective controls. Therefore, co-inoculation of B. anthina and A. awamori showed better performance in stimulating plant growth than that in inoculation of each strain alone. However, assessment period of the present study being short, we recommend in engaging further experimentation under field conditions in order to test the suitability of the strains to be used as bio-inoculants.

• Journal of the Earthquake Engineering Society of Korea
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• v.12 no.1
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• pp.43-55
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• 2008

The complexity of determining strain associated with shear modulus and damping ratio in torsional tests has been resolved by means of several approaches. Particularly, the modified equivalent radius approach is adequate to when generating the plots of equivalent radius ratio versus strain more effectively over any range of strains in resonant column and torsional shear (RC/TS) tests. The modified equivalent radius approach was applied for hyperbolic, modified hyperbolic, and Ramberg-Osgood models in evaluating damping ratio. Results showed that using a single value of equivalent radius ratio based on conventional equivalent radius approach is not appropriate. A new model was developed to consider the soil damping behavior at small strains as well as hysteretic damping and it was attempted to determine adjustments are required in evaluating strain associated damping when combining the two damping components.

• Journal of the Korean Geotechnical Society
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• v.26 no.8
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• pp.5-14
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• 2010

To simulate two-dimensional plane-strain conditions in the laboratory model test, the side frictional resistance between the soil and thick glass or plastic sheet of the soil container should be reduced as much as possible. However, in fact this side friction cannot be removed completely. In this paper, the ground model simulated as a multi-sized aluminium rod mixture was introduced to get rid of the side frictional resistance and applied to the laboratory shear box test. In addition, an application of the close range photogrammetric technique to the shear box test was validated. As a result, it was found that a mean value of dilation angle from the close range photogrammetry was close to the dilation angle defined by the curve of shear strain vs. volumetric strain.

• KSBB Journal
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• v.27 no.2
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• pp.75-85
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• 2012

Although, microbial arsenic mobilization by dissimilatory arsenate-reducing bacteria (DARB) and the practical use to the removal technology of arsenic from contaminated soil are expected, most previous research mainly has been focused on the geochemical circulation of arsenic. Therefore, in this review we summarized the previously reported DARB to grasp the characteristic for bioremediation of arsenic. Evidence of microbial growth on arsenate is presented based on isolate analyses, after which a summary of the physiology of the following arsenate-respiring bacteria is provided: Chrysiogenes arsenatis strain BAL-$1^T$, Sulfurospirillum barnesii, Desulfotomaculum strain Ben-RB, Desulfotomaculum auripigmentum strains OREX-4, GFAJ-1, Bacillus sp., Desulfitobacterium hafniense DCB-$2^T$, strain SES-3, Citrobacter sp. (TSA-1 and NC-1), Sulfurospirillum arsenophilum sp. nov., Shewanella sp., Chrysiogenes arsenatis BAL-$1^T$, Deferribacter desulfuricans. Among the DARB, Citrobacter sp. NC-1 is superior to other dissimilatory arsenate-reducing bacteria with respect to arsenate reduction, particularly at high concentrations as high as 60 mM. A gram-negative anaerobic bacterium, Citrobacter sp. NC-1, which was isolated from arsenic contaminated soil, can grow on glucose as an electron donor and arsenate as an electron acceptor. Strain NC-1 rapidly reduced arsenate at 5 mM to arsenite with concomitant cell growth, indicating that arsenate can act as the terminal electron acceptor for anaerobic respiration (dissimilatory arsenate reduction). To characterize the reductase systems in strain NC-1, arsenate and nitrate reduction activities were investigated with washed-cell suspensions and crude cell extracts from cells grown on arsenate or nitrate. These reductase activities were induced individually by the two electron acceptors. Tungstate, which is a typical inhibitory antagonist of molybdenum containing dissimilatory reductases, strongly inhibited the reduction of arsenate and nitrate in anaerobic growth cultures. These results suggest that strain NC-1 catalyzes the reduction of arsenate and nitrate by distinct terminal reductases containing a molybdenum cofactor. This may be advantageous during bioremediation processes where both contaminants are present. Moreover, a brief explanation of arsenic extraction from a model soil artificially contaminated with As (V) using a novel DARB (Citrobacter sp. NC-1) is given in this article. We conclude with a discussion of the importance of microbial arsenate reduction in the environment. The successful application and use of DARB should facilitate the effective bioremediation of arsenic contaminated sites.

• Proceedings of the Korean Geotechical Society Conference
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• 2000.11a
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• pp.295-302
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• 2000

Earthquakes not only produce additional load on the structures and underlying soil, but also change the strength characteristics of the soil. Therefore, in order to analyze soil structures for stability, the behaviour after earthquake must be considered. In this paper, a series of cyclic triaxial tests and monotonic triaxial tests were carried out to investigate the undrained shear strength and liquefaction strength characteristics of Nak-Dong River sand soils which were subjected to cyclic loading. The sample was consolidated in the first stage and then subjected to stress controlled cyclic loading with 0.1Hz. After the cyclic loading, the cyclic-induced excess pore water pressure was dissipated by opening the drainage valve and the sample was reconsolidated to the initial effective mean principal stress(p/sub c/'). After reconsolidation, the monotonic loading or cyclic loading were applied to the specimen. In the results, the undrained shear strength and liquefaction strength characteristics depended on the pore pressure ratio(Ur=U/p/sub c/'). The volume change following reconsolidation can be a function of cyclic-induced excess pore water pressure and the maximum double amplitude of axial strain.

• Geomechanics and Engineering
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• v.5 no.3
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• pp.241-261
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• 2013

The bearing mechanism of pile during installation and loading process which controls the deformation and distribution of strain and stress in the soil surrounding pile tip is complex and full of much uncertainty. It is pointed out that particle crushing occurs in significant stress concentrated region such as the area surrounding pile tip. The solution to this problem requires the understanding and modeling of the mechanical behavior of granular soil under high pressures. This study aims to investigate the sand behavior around pile tip considering the characteristics of sand crushing. The numerical analysis of model pile loading test under different surcharge pressure with constitutive model for sand crushing is presented. This constitutive model is capable of predicting the dilatancy of soil from negative to positive under low confining pressure and only negative dilatancy under high confining pressure. The predicted relationships between the normalized bearing stress and normalized displacement are agreeable with the experimental results during the entire loading process. It is estimated from numerical results that the vertical stress beneath pile tip is up to 20 MPa which is large enough to cause sand to be crushed. The predicted distribution area of volumetric strain represents that the distributed area shaped wedge for volumetric contraction is beneath pile tip and distributed area for volumetric expansion is near the pile shaft. It is demonstrated that the finite element formulation incorporating a constitutive model for sand with crushing is capable of producing reasonable results for the pile loading problem.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.9 no.3
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• pp.133-141
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• 1989

Lightly overconsolidated clays are commonly anisotropic, and exibit substantial ranges of approximately linear behavior at stress levels which do not produce yielding. The theory of cross-anisotropic elasticity is adopted to predict the stress-strain behavior of such an anisotropic soil. Equivalent elastic parameters $A^*$ and $B^*$ which express the relationships of stress and strain in the theory have been proposed. It is shown that constitutive relationships derived from the theory represents well the mechanical response of anisotropic soil.

• Magazine of the Korean Society of Agricultural Engineers
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• v.38 no.6
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• pp.64-73
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• 1996

The three-dimensional strength behavior of compacted decomposed granite soil was studied using cubical triaxial tests with independent control of the three principal stresses. All specimens were loaded under conditions of principal stress direction fixed and aligned with the directions of compacted plane. For comparable test conditions, the major principal strain and volume strain to failure were smallest when the major principal stress acted perpendicular to the compacted plane. The opposite extremes were obtained when the major principal stress acted parallel to the compacted plane. In cubical triaxial tests with same b values and with ${\theta}$ values in one of three sectors of the octahedral plane, independent of the range of ${\theta}$, higher friction angles are obtained in tests with b greater than in triaxial compression tests in which b 0.0, Comparison between the results of the drained cubical triaxial tests on lksan compacted decomposed granite soil and the cross section of the Mohr-Coulomb failure surface as well as the cross section of the Mohr-Coulomb failure surface were made. Lade's isotropic failure criterion based on vertical specimens overestimates the strengths for tests performed with values of 0 between 90˚ and 1 50˚ the Mohr-Coulomb criterion generally underestimates the strengths of tests performed with values of ${\theta}$ between $0^{\circ}$ and $180^{\circ}$ except around the $120^{\circ}$.

• Korean Journal of Agricultural Science
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• v.37 no.2
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• pp.255-260
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• 2010

Rusty root, the browning disease on ginseng, decreases quality and value. Recent studies indicated that endophytic bacteria could be a possible cause of rusty root. Actinomycetes antagonistic to the rusty-root-causing bacteria were isolated from soil. Twenty nine out of 932-isolates of Actinomycetes from soil showed antibacterial activity against Agrobacterium tumefaciens and Pseudomonas veronii an endophytic isolate in ginseng. The strongest antibacterial strain(ATO4O104) was classified based on 16S rDNA sequence. The Actinomycetes strain, ATO4O104, isolated in soil of USA volcano national park was identified as Streptomyces adephospholyticus. To test plant toxicity, radish seeds were sprouted with the culture of S. adephospholyticus and it did not show any harmful effect. The butanol partition out of n-hexane, ethyl acetate, butanol, and water partions showed the highest antibacterial activity.