• Journal of Microbiology and Biotechnology
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• v.26 no.2
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• pp.213-225
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• 2016

To reduce attrition in drug development, it is crucial to consider the development and implementation of translational phenotypic assays as well as decipher diverse molecular mechanisms of action for new molecular entities. High-throughput fluorescence and confocal microscopes with advanced analysis software have simplified the simultaneous identification and quantification of various cellular processes through what is now referred to as high-content screening (HCS). HCS permits automated identification of modifiers of accessible and biologically relevant targets and can thus be used to detect gene interactions or identify toxic pathways of drug candidates to improve drug discovery and development processes. In this review, we summarize several HCS-compatible, biochemical, and molecular biology-driven assays, including immunohistochemistry, RNAi, reporter gene assay, CRISPR-Cas9 system, and protein-protein interactions to assess a variety of cellular processes, including proliferation, morphological changes, protein expression, localization, post-translational modifications, and protein-protein interactions. These cell-based assay methods can be applied to not only 2D cell culture but also 3D cell culture systems in a high-throughput manner.

• Natural Product Sciences
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• v.22 no.1
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• pp.64-69
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• 2016

A method for simultaneously identifying antioxidative compounds was developed using time-based LC-MS coupled with DPPH assay regardless of the time consuming process. The methanolic extract of Polygonum aviculare (Polygonaceae) showed significant DPPH radical scavenging activity. Time-based DPPH assay for simultaneous identification of active compounds from the extracts of P. aviculare was used. Major peaks of ethyl acetate fraction of P. aviculare showed high DPPH radical scavenging activity. A simple phenolic compound (1) and six flavonoids (2-7) were isolated from the ethyl acetate fraction of P. aviculare by silica gel and sephadex LH-20 column chromatography. The structures of seven compounds were determined to be protocatechuic acid (1), catechin (2), myricitrin (3), epicatechin-3-O-gallate (4), avicularin (5), quercitrin (6), and juglanin (7) based on the analysis of the $^1H$-NMR, $^{13}C$-NMR and ESI-MS data. All compounds exhibited significant antioxidant activity on DPPH assay and active compounds were well correlated with predicted one.

• International conference on construction engineering and project management
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• 2024.07a
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• pp.1073-1080
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• 2024

The imperative for real-time automatic construction progress monitoring (ACPM) to avert project delays is widely acknowledged in construction project management. Current ACPM methodologies, however, face a challenge as they rely on collecting data from construction sites and processing it offline for progress analysis. This delayed approach poses a risk of late identification of critical construction issues, potentially leading to rework and subsequent project delays. This research introduces a real-time construction progress monitoring framework that integrates cutting-edge semantic Simultaneous Localization and Mapping (SLAM) techniques. The innovation lies in the framework's ability to promptly identify structural components during site inspections conducted through a robotic system. Incorporating deep learning models, specifically those employing semantic segmentation, enables the system to swiftly acquire and process real-time data, identifying specific structural components and their respective locations. Furthermore, by seamlessly integrating with Building Information Modeling (BIM), the system can effectively evaluate and compare the progress status of each structural component. This holistic approach offers an efficient and practical real-time progress monitoring solution for construction projects, ensuring timely issue identification and mitigating the risk of project delays.

• Research in Plant Disease
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• v.26 no.4
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• pp.264-271
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• 2020

We have developed a simultaneous diagnostic method that can identify both the species of thrips and tomato spotted wilt virus (TSWV) that are problematic in chrysanthemum plants. This is a method of amplifying DNA by performing reverse transcription-polymerase chain reaction by simultaneously adding primers specific to TSWV coat protein (N) gene and primers specific to the internal transcribed spacer 2 region of Frankliniella occidentalis and F. intonsa using total nucleic acid extracted from one thrips. The sizes of DNA fragments for TSWV, F. occidentalis, and F. intonsa were 777, 287, and 367 bp, respectively. These results showed species identification of thrips and whether thrips carrying TSWV can be simultaneously confirmed. Further usefulness of the simultaneous diagnostic method was made from greenhouse survey at chrysanthemum greenhouses in Taean (Chungcheongnam-do) and Changwon (Gyeongsangnam-do) to investigate the identification of thrips species and the rate of thrips carrying TSWV. Of thrips collected from the greenhouses, 83.7% thrips was F. occidentalis and 72.9% F. occidentalis carried TSWV in Taean. Similarly, the diagnostic method showed that 92.2% thrips was F. occidentalis and 84.0% F. occidentalis carried TSWV in Changwon. These results confirm that F. occidentalis is a dominant thrips species and the thrips species plays a crucial role in the transmission of TSWV in chrysanthemum plants in the greenhouses. Taken together, this study showed a simple diagnostic method for thrips identification and epidemiological studies of the timing and spread of TSWV through thrips in chrysanthemum greenhouses in South Korea.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.2
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• pp.209-213
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• 1997

Since Houttuynia cordata is well known as a medicinal herb, due to its antibacterial activity on various microorganisms, present investigation was performed to identify the flavor compounds for volatile essential oil. Volatile essential oil was collected by simultaneous distillation-extraction(SDE), and then the oil components were separated on HP-5 capilliary column$(25m{\times}0.25mm\; i.d.)$ and identified those components by GC-MS. Fifty two compounds were isolated from the volatile essential oil of Houttuynia cordata and forty four were positively identified by GC-MS. The volatile compounds were composed mainly of terpenoids(25 classes), aldehydes(7 classes), alcohols(4 classes), ketones(3 classes), acids(1 class) and miscellaneous compounds(4 classes). Of these, the major compounds were ${\beta}-myrcene$, ${\beta}-ocimene$, decanal, 2-undecanone and geranyl propionate.

• Journal of Ginseng Research
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• v.34 no.1
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• pp.41-46
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• 2010

Expensive herbs such as ginseng are always a possible target for fraudulent labeling. New mountain ginseng strains have occasionally been found deep within mountain areas and commercially traded at exorbitant prices. However, until now, no scientific basis has existed to distinguish such ginseng from commonly cultivated ginseng species other than by virtue of being found within deep mountain areas. Polymerase chain reaction (PCR) analysis of the internal transcribed spacer has been shown to be an appropriate method for the identification of the most popular species (Panax ginseng) in the Panax ginseng genus. A single nucleotide polymorphism (SNP) has been identified between three newly found mountain ginseng (KGD4, KGD5, and KW1) and already established Panax species. Specific PCR primers were designed from this SNP site within the sequence data and used to detect the mountain ginseng strains via multiplex PCR. The established multiplex-PCR method for the simultaneous detection of newly found mountain ginseng strains, Korean ginseng, and foreign ginseng in a single reaction was determined to be effective. This study is the first report of scientific discrimination of "mountain ginsengs" and describes an effective method of identification for fraud prevention and for uncovering the possible presence of other, cheaper ginseng species on the market.

• Smart Structures and Systems
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• v.34 no.2
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• pp.97-116
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• 2024

The standard l₁-norm regularization is recently introduced for impact force identification, but generally underestimates the peak force. Compared to l₁-norm regularization, l_p-norm (0 ≤ p < 1) regularization, with a nonconvex penalty function, has some promising properties such as enforcing sparsity. In the framework of sparse regularization, if the desired solution is sparse in the time domain or other domains, the under-determined problem with fewer measurements than candidate excitations may obtain the unique solution, i.e., the sparsest solution. Considering the joint sparse structure of impact force in temporal and spatial domains, we propose a general l_p-norm (0 ≤ p < 1) regularization methodology for simultaneous identification of the impact location and force time-history from highly incomplete measurements. Firstly, a nonconvex optimization model based on l_p-norm penalty is developed for regularizing the highly under-determined problem of impact force identification. Secondly, an iteratively reweighed l₁-norm algorithm is introduced to solve such an under-determined and unconditioned regularization model through transforming it into a series of l₁-norm regularization problems. Finally, numerical simulation and experimental validation including single-source and two-source cases of impact force identification are conducted on plate structures to evaluate the performance of l_p-norm (0 ≤ p < 1) regularization. Both numerical and experimental results demonstrate that the proposed l_p-norm regularization method, merely using a single accelerometer, can locate the actual impacts from nine fixed candidate sources and simultaneously reconstruct the impact force time-history; compared to the state-of-the-art l₁-norm regularization, l_p-norm (0 ≤ p < 1) regularization procures sufficiently sparse and more accurate estimates; although the peak relative error of the identified impact force using l_p-norm regularization has a decreasing tendency as p is approaching 0, the results of l_p-norm regularization with 0 ≤ p ≤ 1/2 have no significant differences.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.37 no.2
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• pp.62-69
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• 2014

The capability and cost effectiveness of UUV and USV bring to underwater survey, target detection and identification operations have been widely demonstrated and accepted in recent years. Future USV systems may deploy UUVs to gain the advantage of higher area coverage rates through multiple and simultaneous operations. In this paper, we present an architecture of USV and UUV for mine countermeasure with results of measures on effectiveness.

• Journal of Microbiology and Biotechnology
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• v.21 no.1
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• pp.89-92
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• 2011

The routine identification and differentiation of Brucella species is a time-consuming and labor-intensive process, which frequently places personnel at risk of laboratory-acquired infection. Here, we describe the development of a rapid multiplex PCR assay for the confirmation of presumptive Brucella isolates. The assay was able to identify and differentiate major human pathogens, namely B. abortus, B. melitensis, and B. suis, in a single test of less than an hour and a half.

• Journal of Advanced Marine Engineering and Technology
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• v.27 no.5
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• pp.633-640
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• 2003

The present experimental study is focused on the application of multi-point simultaneous measurement by PIV(Particle Image Velocimetry) to guide vane region within diffuser pump. Various different kinds of rotational velocity were selected as experimental condition. Optimized cross correlation Identification to obtain velocity vectors is implemented with direct calculation of correlation coefficients. Fine optical setup concerned with PIV performance is arranged for the accurate PIV measurement of high-speed complex flow. Variable flow pattern are represented quantitatively at the stator region.