Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Rapid Multiplex PCR Assay for the Simultaneous Detection of the Brucella Genus, B. abortus, B. melitensis, and B. suis

  • Kumar, Sanjay (Division of Biotechnology, Defence Research and Development Establishment) ;
  • Tuteja, Urmil (Division of Microbiology, Defence Research and Development Establishment) ;
  • Sarika, Kumari (Division of Microbiology, Defence Research and Development Establishment) ;
  • Singh, Dhirendra Kumar (Division of Veterinary Public Health, Indian Veterinary Research Institute) ;
  • Kumar, Ashok (Division of Veterinary Public Health, Indian Veterinary Research Institute) ;
  • Kumar, Om (Division of Biotechnology, Defence Research and Development Establishment)
  • Received : 2010.07.27
  • Accepted : 2010.09.29
  • Published : 2011.01.28
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Abstract

The routine identification and differentiation of Brucella species is a time-consuming and labor-intensive process, which frequently places personnel at risk of laboratory-acquired infection. Here, we describe the development of a rapid multiplex PCR assay for the confirmation of presumptive Brucella isolates. The assay was able to identify and differentiate major human pathogens, namely B. abortus, B. melitensis, and B. suis, in a single test of less than an hour and a half.

Keywords

References

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