• Title/Summary/Keyword: root response

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A QUANTITATIVE ANALYSIS OF THE IMMUNOCOMPETENT CELLS IN PERIAPICAL LESIONS OF THE HUMAN TEETH (치근단 병소에서 면역적격세포의 분포에 관한 연구)

  • Yoon, Tai-Cheol;Kim, Jin;Park, Dong-Soo
    • Restorative Dentistry and Endodontics
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    • v.17 no.1
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    • pp.55-68
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    • 1992
  • Periapical lesions are developed as a result of inflammatory response to irritants from root canal system. Clinicians remove these irritants from root canal system and seal the root canal space to induce healing of the periapical lesions. Immunopathologic responses may play an important role in development and progression of periapical lesions and periapical lesions contain immunocompetent cells. The purposes of the present study were to analys and to compare the distribution of the immunocompetent cells in the human periapical lesions according to the stage of endodontic treatment using indirect immunoperoxdase technique. Obtained 94 human periapical lesions were devided into four groups: Group 1 : no endodontic treatment(28 samples) Group 2 : root canal enlarged and irrigated(28 samples) Group 3 : root canal filled(29 samples) Group 4: unknown(9 samples) Monoclonal antibodies to examine target cells were UCHL-1 for T lymphocytes(1 : 200, Dakopatt, Denmark), L26 for B lymphocytes(1 : 200, Dakopatt, Denmark), OPD4 for helper T lymphocytes(l : 200, Dakopatt, Denmark) and alpha-1-antichymotrypsin for macrophages(l : 2000, Dakopatt, Denmark). The following results were obtained : 1. All the periapical lesions studied were infiltrated by T lymphocytes, plasma cells, B lymphocytes, and macrophages. T lymphocytes were more infiltrated than B lymphocytes, and B lymphocytes and macrophages were less infiltrated than T lymphocytes and plasma cells(P<0.05 : Oneway ANOVA test). 2. In untreated group and canal irrigated and enlarged group of all the periapical lesions, helper T lymphocytes were predominently infiltrated(P>0.05 : Oneway ANOVA test). 3. In canal filled groups of all lesions except periapical cyst, plasma cells were predominently infiltrated. But, in canal filled group of periapical cyst, helper T lymphocytes were the predominent cells(P>0.05 : Oneway ANOVA test). The above results shows that the immunologic responses play important role in pathogenesis of periapical lesions and the immunologic response involved undergoes certain changes after endodontic therapy.

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Failure mechanisms of a rigid-perfectly plastic cantilever with elastic deformation at its root subjected to tip pulse loading

  • Wang, B.
    • Structural Engineering and Mechanics
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    • v.2 no.2
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    • pp.141-156
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    • 1994
  • In this paper, the effect of material elasticity was evaluated through a simple model as proposed by Wang and Yu (1991), for yield mechanisms of a cantilever beam under tip pulse loading. The beam was assumed rigid-perfectly plastic but instead of the usual fully clamped constraints at its root, an elastic-perfectly plastic rotational spring was introduced there so the system had a certain capacity to absorb elastic energy. Compared with a rigid-perfectly plastic beam without a spring root, the present beam-spring model showed differences in the initial plastic hinge position and the minimum magnitude of the dynamic force needed to produce a plastic failure. It was also shown that various failure responses may happen while the hinge travels along the beam segment towards the root, rather than a unique response mode as in a rigid perfectly plastic analysis.

The Rate of Soil Respiration in Populus maximowiczii Stand on Volcano Mt. Usu, Northern Japan

  • Moon, Hyun-Shik;Masahiro, Haruki
    • The Korean Journal of Ecology
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    • v.24 no.2
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    • pp.87-91
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    • 2001
  • The response of respiration rates of root, Ao layer and mineral soil to varying environmental factors was studied in Popuius maximowiczii stand (25-year-old) during the growing season of 1997. Soil temperature showed a pronounced seasonal course, in contrast to soil moisture. The mineral soil respiration was high in August, and root and Ao layer respiration, were high in July. An exponential equation best described the relationships between soil temperature and mineral soil respiration, and total soil respiration (r=0.95 and 0.92, p<0.001), respectively. In P. maximowiczii stand, soil respiration rates were reduced by about 19% after removal of the Ao layer, and by about 30% after removal of living root. Therefore, mineral soil respiration seemed to contribute gretly to the total soil respiration (50%).

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Optimization of the Sucrose and Ion Concentrations for Saikosaponin Production in Hairy Root Culture of Bupleurum falcatum

  • Ahn, Jun-Cheul;Chong, Won-Seog;Kim, Young-Soon;Hwang, Baik
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.2
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    • pp.121-126
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    • 2006
  • Saikosaponin productivity was examined in a Bupleurum falcatum L. BFHR2 hairy root culture in response to changes in the sucrose content $(2{\sim}8%)$, nitrogen content $(0{\sim}250mM\;NH_4NO_3)$, phosphate content $(0{\sim}12mM\;NaH_2PO_4)$, and the potassium content $(0{\sim}87.2 mM\; KCl)$ of the culture media. We found that the conditions for maximal saikosaponin production differed from those for optimal root growth. Highest saikosaponin yield was achieved for 8% sucrose, 62mM $NH_4NO_3$ 1.2 mM $NaH_2PO_4$, and 0.5mM KCl.

Spinach Root-Tissue Based Amperometric Biosensor for the Determination of Hydrogen Peroxide (시금치 뿌리 조직 바이오센서를 이용한 과산화수소의 정량)

  • Lee, Beom-Gyu;Yoon, Kil-Joong;Kwon, Hyo-Shik
    • Analytical Science and Technology
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    • v.13 no.3
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    • pp.315-322
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    • 2000
  • The response characteristics of the bioelectrode developed by the co-immobilization of spinach root tissue and ferrocene in a carbon paste matrix for the amperometric determination of hydrogen peroxide were evaluated. In the range of electrode potential examined (-0.3~0.0V vs. Ag/AgCl). the response time was relatively short ($t_{95%}=11.8$ sec) and it responded in the wide range of pH. Also, its detection limit was $2.25{\times}10^{-6}M$ (S/N=3) and a relative standard deviation of the measurements which were repeated 15 times using $1.0{\times}10^{-3}M$ hydrogen peroxide was 1.87%. The bioelectrode sensitivity decreased to 40% of the original value in 19 days of continuous use.

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Intraspecific Functional Variation of Arbuscular Mycorrhizal Fungi Originated from Single Population on Plant Growth

  • Lee, Eun-Hwa;Ka, Kang-Hyeon;Eom, Ahn-Heum
    • 한국균학회소식:학술대회논문집
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    • 2014.10a
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    • pp.48-48
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    • 2014
  • Arbuscular Mycorrhizal Fungi(AMF) is widespread symbiont forming mutualistic relationship with plant root in terrestrial forest in ecosystem. They provide improved absorption of nutrient and water, and enhance the resistance against plant pathogen or polluted soil, therefore AM fungi are important for survival and maintaining of individual or community of plant. For last decade, many studies about the functional variation of AM fungi on host plant growth response were showed that different geographic isolates, even same species, have different effect on host plant. However, little was known about functional variation of AM fungal isolates originated single population, which provide important insight about intraspecific diversity of AMF and their role in forest ecosystem. In this study, four AM fungal isolates of Rhizophagus clarus were cultured in vitro using transformed carrot (Daucus carota) root and they showed the difference between isolates in ontogenic characteristics such as spore density and hyphal length. The plant growth response by mycorrhizas were measured also. After 20 weeks from inoculation of these isolates to host plants, dry weight, Root:Shoot ratio, colonization rates and N, P concentration of host plant showed host plant was affected differently by AM fungal isolates. This results suggest that AM fungi have high diversity in their functionality in intraspecific level, even in same population.

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Selection of an Optimal Welding Condition for Back Bead Formation in GMA Root Pass Welding (GMA 초층용접에서 이면비드 생성을 위한 최적용접조건의 선정)

  • Yun, Young-Kil;Kim, Jae-Woong;Yun, Seok-Chul
    • Journal of Welding and Joining
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    • v.28 no.5
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    • pp.86-92
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    • 2010
  • In GMAW processes, bead geometry is a criterion to estimate welding quality. Bead geometry is affected by welding current, arc voltage, welding speed, shielding gas and so on. Thus the welding condition has to be selected carefully. In this paper, an experimental method for the selection of optimal welding condition was proposed in the root pass welding which was done along the GMA V-grooved butt weld joint. This method uses the response surface analysis in which the width and height of back bead were chosen as the quality variables of the weld. The overall desirability function, which is the combined desirability function for the two quality variables, was used as the objective function for getting the optimal welding condition. Through the experiments, the target values of the back bead width and the height were chosen as 4mm and 1mm respectively for the V-grooved butt weld joint. From a series of welding test, it was revealed that a uniform weld bead can be obtained by adopting the optimal welding condition which was determined according to the method proposed.

Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP

  • Kamran, Muhammad;Javed, Nazir;Ullah, Ihsan;Nazir, Shahid;Jamil, Shakra;Iqbal, Muhammad Zafar;Abbas, Huma;Khan, Sajid Aleem;Haq, Muhammad Ehetisham ul
    • The Plant Pathology Journal
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    • v.35 no.1
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    • pp.51-62
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    • 2019
  • A great variable response was observed when PP-3 and PP-J encumbered with 116 populations of root knot nematode (RKN) at two different temperatures ($25{\pm}2^{\circ}C$ and $30{\pm}2^{\circ}C$) and concentrations ($10^4$ and $10^5$ spores/ml). The PCR reaction amplified intergenic region between cytochrome oxidase subunit II gene (COII) and large subunit of rRNA gene (lrRNA) of the mitochondrial genome of different RKN species. The primer C2F3 and 1108 identified M. incognita with the highest frequency (52.6%) followed by M. javanica (36.8%) and M. arenaria (10.5%). The sizes of PCR products were 1.7 kb for M. incognita and M. javanica populations while populations of M. arenaria produced 1.1 kb fragment. The digestion with Hinf I yielded three different fragment length patterns on 1.5 % agarose gel. From current research it is concluded that intra-Meloidogyne genetic variability exist in RKN populations which have better encumbrance with P. penetrans.

Antimicrobial Property of $(+)-Lyoniresinol-3{\alpha}-O-\beta-D-Glucopyranoside$ Isolated From the Root Bark of Lycium chinense Miller Against Human Pathogenic Microorganisms

  • Lee Dong Gun;Jung Hyun Jun;Woo Eun-Rhan
    • Archives of Pharmacal Research
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    • v.28 no.9
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    • pp.1031-1036
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    • 2005
  • [ $(+)-Lyoniresinol-3{\alpha}-O-\beta-D-glucopyranoside$ ] (1) was isolated from an ethyl acetate extract of the root bark from Lycium chinense Miller, and its structure was determined using 1D and 2D NMR spectroscopy including DEPT, HMQC, and HMBC. $(+)-Lyoniresinol-3{\alpha}-O-\beta-D-glucopyranoside$ exhibited potent antimicrobial activity against antibiotic-resistant bacterial strains, methicillin-resistant Staphylococcus aureus (MRSA) isolated from patients, and human pathogenic fungi without having any hemolytic effect on human erythrocytes. In particular, compound 1 induced the accumulation of intracellular trehalose on C. albicans as stress response to the drug, and disrupted the dimorphic transition that forms pseudo-hyphae caused by the pathogenesis. This indicates that $(+)-Lyoniresinol-3{\alpha}-O-\beta-D-glucopyranoside$ has excellent potential as a lead compound for the development of antibiotic agents.

A Study on Vibration Reduction of Local Structure of Medium-speed Diesel Engine (중속 디젤엔진 국부 구조물의 진동저감 연구)

  • Jung, Kun-Hwa;Lee, Jun-Ho;Son, Jung-Ho;Ryoo, Young-Seok
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2011.10a
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    • pp.257-261
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    • 2011
  • The failures of local structure of marine medium-speed diesel engine occurred in durability test. The vibration response of the whole engine was in an acceptance level, but the local structures were broken. ODS test and vibration measurement were carried out in order to investigate the root cause of durability problem. These tests revealed that the root cause of failures was excessive vibration by $4.5^{th}$ resonance between engine body and local structure. The best solution to reduce the vibration response is to change the type of mount. After a rigid mount was replaced by a flexible mount, maximum vibration level dropped to 72%.

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