Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Spinach Root-Tissue Based Amperometric Biosensor for the Determination of Hydrogen Peroxide

시금치 뿌리 조직 바이오센서를 이용한 과산화수소의 정량

  • 이범규 (조선대학교 자연과학대학 물리화학부) ;
  • 윤길중 (청주대학교 이공대학 화학과) ;
  • 권효식 (충북대학교 사범대학 과학교육연구소)
  • Received : 2000.03.16
  • Published : 2000.06.25
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Abstract

The response characteristics of the bioelectrode developed by the co-immobilization of spinach root tissue and ferrocene in a carbon paste matrix for the amperometric determination of hydrogen peroxide were evaluated. In the range of electrode potential examined (-0.3~0.0V vs. Ag/AgCl). the response time was relatively short ($t_{95%}=11.8$ sec) and it responded in the wide range of pH. Also, its detection limit was $2.25{\times}10^{-6}M$ (S/N=3) and a relative standard deviation of the measurements which were repeated 15 times using $1.0{\times}10^{-3}M$ hydrogen peroxide was 1.87%. The bioelectrode sensitivity decreased to 40% of the original value in 19 days of continuous use.

시금치의 뿌리 조직과 ferrocene을 탄소반죽 전극에 고정하여 과산화수소를 정량할 수 있는 전극을 만들었다. Ag/AgCl 전극에 대하여 -0.3~0.0V의 전위의 범위에서 전극의 감도를 관찰하였으며 전극의 감응시간은 11.8sec로 나타났다. 전극의 검출한계는 $2.25{\times}10^{-6}M$ 이었으며, $1.0{\times}10^{-3}M$ 과산화수소를 이용해 15회 반복 측정한 결과, 상대표준편차는 1.87% 이었다. 효소전극의 감도는 19일 사용 후 40%로 감소하였다.

Keywords

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