• Asian-Australasian Journal of Animal Sciences
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• 제19권10호
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• pp.1394-1398
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• 2006

The complete mtDNA D-loop regions of Japanese and Korean cattle were analyzed for their mtDNA variations and genetic relationships. Sequencing the 30 Higo substrain and 30 Tosa substrain of Japanese Brown, respectively 12 and 17 distinct Bos haplotypes were identified from 77 polymorphic nucleotide sites. In order to focus on the relationships among Japanese and Korean cattle, two types of phylogenetic tree were constructed using individual sequences; first, a neighbor-joining tree with all sequences and second, reduced median networks within each Japanese and Korean cattle group. The trees revealed that two major mtDNA haplotype groups, T3 and T4, were represented in Japanese and Korean cattle. The T4 haplogroup predominated in Japanese Black and Japanese Brown cattle (frequency of 43.3-66.7%), while the T3 haplogroup was predominant (83.3%) and T4 was represented only twice in the Korean cattle. The results suggested that the mitochondrial origins of Japanese Brown were Japanese ancient cattle as well as Japanese Black in despite of the considerable introgression of Korean and European cattle into Japanese Brown.

• 한국축산식품학회지
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• 제27권3호
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• pp.357-362
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• 2007

Nine brand populations of Hanwoo cattle were characterized using 11 microsatellite DNA markers. The studied populations were: Ansung, Yangpyang, DaeGwanryeng, Palkongsangkangwoo, Hoengseong, Jangsu, Sumjinkang, Hadong, Nam-hae. The observed heterozygosity, expected heterozygosity, and polymorphism information content were calculated. Allele frequencies were calculated and used for the characterization of each brand population and to study their genetic relationships. Genetic distances were estimated using Nei's DA genetic distance and the resultant DA matrix was used in the construction of phylogenetic trees. The NJ tree showed that Ansung and Yangpyang, Sumjinkang and Jangsu, Namhae and Ha-Dong are closely related and are considered to have undergone genetic exchange within the same locale. This study will contribute to the local Hanwoo brand industry.

• Animal cells and systems
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• 제13권4호
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• pp.453-460
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• 2009

Morphological and molecular classifications were attempted in an effort to establish species-specific classifications of three species of the genus Pholis in Korea; these species were subjected to morphological and molecular methodologies using body measurements, RFLP, RAPD, and phylogenetic trees using the nucleotide sequences of mitochondrial 16S and 12S ribosomal DNAs, cytochrome c oxidase I, and cytochrome b. The data demonstrated that the three species of genus Pholis are distinct from each other, both morphologically and genetically.

• ALGAE
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• 제36권3호
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• pp.175-193
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• 2021

This study provides the first report of the presence of Coolia malayensis in the Mediterranean Sea, co-occurring with C. monotis. Isolated strains from the Gulf of Gabès, Tunisia (South-eastern Mediterranean) were identified by morphological characterization and phylogenetic analysis. Examination by light and scanning electron microscopy revealed no significant morphological differences between the Tunisian isolates and other geographically distant strains of C. monotis and C. malayensis. Phylogenetic trees based on ITS1-5.8S-ITS2 and D1-D3/28S rDNA sequences showed that C. monotis strains clustered with others from the Mediterranean and Atlantic whereas the C. malayensis isolate branched with isolates from the Pacific and the Atlantic, therefore revealing no geographical trend among C. monotis and C. malayensis populations. Ultrastructural analyses by transmission electron microscopy revealed the presence of numerous vesicles containing spirally coiled fibers in both C. malayensis and C. monotis cells, which we speculate to be involved in mucus production.

• 식물분류학회지
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• 제51권3호
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• pp.326-331
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• 2021

The complete chloroplast genome of Zoysia macrostachya Franch. & Sav. isolated in Korea is 135,902 bp long (GC ratio is 38.4%) and has four subregions; 81,546 bp of large single-copy (36.3%) and 12,586 bp of small single-copy (32.7%) regions are separated by 20,885 bp of inverted repeat (44.1%) regions, including 130 genes (83 protein-coding genes, eight rRNAs, and 39 tRNAs). Thirty-nine single nucleotide polymorphisms and 11 insertions and deletion (INDEL) regions were identified from two Z. macrostachya chloroplast genomes, the smallest among other Zoysia species. Phylogenetic trees show that two Z. macrostachya chloroplast genomes are clustered into a single clade. However, we found some incongruency with regard to the phylogenetic position of the Z. macrostachya clade. Our chloroplast genome provides insights into intraspecific variations and species delimitation issues pertaining to the Zoysia species.

• Parasites, Hosts and Diseases
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• 제59권1호
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• pp.89-95
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• 2021

Microcotyle sebastis is a gill monogenean ectoparasite that causes serious problems in the mariculture of the Korean rockfish, Sebastes schlegelii. In this study, we isolated the parasite from fish farms along the coasts of Tongyeong, South Korea in 2016, and characterized its infection, morphology and molecular phylogeny. The prevalence of M. sebastis infection during the study period ranged from 46.7% to 96.7%, and the mean intensity was 2.3 to 31.4 ind./fish, indicating that the fish was constantly exposed to parasitic infections throughout the year. Morphological observations under light and scanning electron microscopes of the M. sebastis isolates in this study showed the typical characteristics of the anterior prohaptor and posterior opisthaptor of monogenean parasites. In phylogenetic trees reconstructed using the nuclear 28S ribosomal RNA gene and the mitochondrial cytochrome c oxidase I gene (cox1), they consistently clustered together with their congeneric species, and showed the closest phylogenetic relationships to M. caudata and M. kasago in the cox1 tree.

• Parasites, Hosts and Diseases
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• 제60권3호
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• pp.195-200
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• 2022

There have been few reports on extra-enteric infections by Blastocystis STs and none have been molecularly identified in samples from human reproductive organs. We report for the first time the identification of 3 different subtypes of Blastocystis (ST1-3) in vaginal and sperm samples, from patients infected with Trichomonas vaginalis. Blastocystis STs were identified by PCR-sequencing and by phylogenetic inferences using 28 vaginal swab samples and 7 sperm samples from patients trichomoniasis. Blastocystis STs were identified in 6 of 28 vaginal swabs (21.4%) and in 3 of 7 sperm samples (42.8%). In both biological samples, STs 1-3 were found; one vaginal sample showed subtype co-infection with ST1 and ST3. High genetic variation was observed in the sequences obtained and no specific clustering in the phylogenetic trees was detected. Most of the haplotypes identified were placed far from the main dispersal centers. Our finding suggested that incorrect cleaning of the genital area or a contamination by combination of anal and vaginal intercourse.

• Mycobiology
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• 제50권4호
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• pp.203-212
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• 2022

Fungi act as important decomposers in the forest environment. They recycle essential nutrients, promote plant growth through mycorrhizal relationships, and act as food for small animals. Samples of 265 indigenous fungal species were collected from Mudeungsan National Park in 2020. These species were identified based on morphological, molecular, and phylogenetic analyses using the internal transcribed spacer (ITS), nuclear large subunit rRNA (LSU), and RNA polymerase II second largest subunit (rpb2) regions. Subsequently, seven species were identified as unrecorded species in Korea: Cordyceps cicadae, Dentocorticium bicolor, Hymenochaete nanospora, Physisporinus crataegi, Rigidoporus piceicola, Russula raoultii, and Scutellinia crinita. This study reveals their detailed macro- and microscopic morphological characteristics with phylogenetic trees to report them as unrecorded species in Korea.

• Journal of Species Research
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• 제13권1호
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• pp.67-88
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• 2024

Macrofungi are essential decomposers in the forest environment. Although more than 70% of the land is mountainous, there has been a lack of research on mushroom diversity in Korea compared to the global species estimation. For this reason, the need for further research became apparent. The surveys were conducted from 2014 to 2022 nationwide. As a result, 15 unrecorded macrofungal species were discovered: Agaricus thiersii, Baorangia alexandri, Boletellus putuoensis, Entoloma bulakhae, Entoloma pygmaeopapillatum, Entoloma subtenuicystidiatum, Gerronema kuruvense, Hyphoderma nudicephalum, Hyphoderma tenue, Macrolepiota subcitrophylla, Mycena jingyinga, Mycena yuezhuoi, Ophiocordyceps vespulae, Scytinostroma acystidiatum, and Steccherinum straminellum. These species are identified based on morphological, molecular, and phylogenetic analyses using the internal transcribed spacer (ITS) region and the nuclear large subunit rRNA (LSU) region. Here, we provided macro- and micro-morphological figures with phylogenetic trees to support 15 species as unrecorded to Korea.

• 식물분류학회지
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• 제36권3호
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• pp.189-207
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• 2006

로젯사철란(G. rosulacea Y. Lee)은 애기사철란과 유사하나 로젯트형의 잎, 짧은 땅속줄기와 서식지 등의 특징에 의해 한국산 사철란속(Goodyera R. Br.) 내의 신종으로 기재된 바 있다. 로젯사철란의 분류군의 실체와 근연종간의 유연관계를 파악하기 위하여 군외군을 포함한 24개의 사철난속 식물을 대상으로 핵 리보좀(ribosomal)의 DNA internal transcribed spacer와 엽록체 DNA의 trnL 구간의 염기서열을 분석하였다. 분류군의 실체와 근연종간의 유연관계는 정렬된 염기서열을 바탕으로 최대절약분석(Maximum parsimony analysis)와 근연결합법(Neibour Joining method)에 의한 계통수 및 고유 표지유전자 여부로 추정하였다. 분석 결과 로젯사철란은 다수의 고유한 표지유전자를 가지며, ITS와 trnL 계통수에서 모두 단계통군을 형성하였다. 로젯사철란과 한국산 사철란속 내 각 분류군간의 유전적 거리(pairwise distance)는 ITS에서 3.49-6.68, trnL에서 5.05-9.53으로서 독립된 종으로 간주하기에 무리가 없었다. 따라서 분자적 결과는 로젯사철란을 사철란속내 독립된 종으로 처리하는 것을 지지하였다. 계통수에서 로젯사철란은 형태적으로 유사한 애기사철란(G. repens)과 동일한 분계조를 형성하였고, 유전적 거리도 조사한 분류군들 중 가장 낮은 값을 나타냈으므로 가장 가까운 근연분류군임을 나타내었다.