Mycobiology

The Korean Society of Mycology (한국균학회)
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Seven Unrecorded Indigenous Fungi from Mudeungsan National Park in Korea

  • Minseo Cho (Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University) ;
  • Sun Lul Kwon (Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University) ;
  • Young Mok Heo (R&I Center, COSMAX BTI) ;
  • Young Min Lee (Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University) ;
  • Hanbyul Lee (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Changmu Kim (Division of Biological and Genetic Resources Assessment, National Institute of Biological Resources) ;
  • Byoung Jun Ahn (Department of Forest Products and Industry, Division of Forest Industrial Materials, National Institute of Forest Science) ;
  • Jae-Jin Kim (Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University)
  • Received : 2022.02.21
  • Accepted : 2022.07.31
  • Published : 2022.08.31
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Abstract

Fungi act as important decomposers in the forest environment. They recycle essential nutrients, promote plant growth through mycorrhizal relationships, and act as food for small animals. Samples of 265 indigenous fungal species were collected from Mudeungsan National Park in 2020. These species were identified based on morphological, molecular, and phylogenetic analyses using the internal transcribed spacer (ITS), nuclear large subunit rRNA (LSU), and RNA polymerase II second largest subunit (rpb2) regions. Subsequently, seven species were identified as unrecorded species in Korea: Cordyceps cicadae, Dentocorticium bicolor, Hymenochaete nanospora, Physisporinus crataegi, Rigidoporus piceicola, Russula raoultii, and Scutellinia crinita. This study reveals their detailed macro- and microscopic morphological characteristics with phylogenetic trees to report them as unrecorded species in Korea.

Keywords

Acknowledgement

The authors thank Editage (www.editage.co.kr) for English language editing.

References

  1. Hawksworth DL, Lucking R. Fungal diversity € revisited: 2.2 to 3.8 million species. Microbiol Spectr. 2017;5:5(4):10.
  2. Hawksworth DL, Sridhar K, Deshmukh S. The macrofungal resource. Advances in macrofungi: diversity, ecology and biotechnology. Boca Raton (FL): CRC Press; 2019. p. 1-9.
  3. Seok SJ, Kim CM, Ka KH, et al. List of mushrooms in Korea. Seoul: Korean Society of Mycology; 2013.
  4. Resources NIoB. Biodiversity of the Korean Peninsula. [Internet]. 2022. Available from: https://species.nibr.go.kr/index.do
  5. Han SW, Cho YR, Shin YU, et al. Avifauna of the Mudeungsan (Mt.) region of Gwangju metropolitan city. J Korean Nat. 2011;4(1):35-43. https://doi.org/10.7229/jkn.2011.4.1.035
  6. Yu SB, Lee SC, Kang HM, et al. Analysis of vegetation structure on the 2nd old trail in Mudeungsan National Park. Korean J Environ Ecol. 2020;34(3):224-234.
  7. Ko MH, Jang SL, Won YJ, Fish distribution characteristics of Mudeungsan National Park. Korean J Environ Ecol. 2018;32(2):154-164. https://doi.org/10.13047/KJEE.2018.32.2.154
  8. Lee JW, Lee S, Kang SH. A study on the flora and its introduced disturbing plants in Damyang area of Mudeungsan National Park, Korea. Korean J Plant Resource. 2021;34:103-113.
  9. Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol. 1993;2(2):113-118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  10. White TJ, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocol. 1990;18:315-322.
  11. Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol. 1990;172(8):4238-4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  12. Hopple JS, Jr, Vilgalys R. Phylogenetic relationships among coprinoid taxa and allies based on data from restriction site mapping of nuclear rDNA. Mycologia. 1994;86(1):96-107. https://doi.org/10.1080/00275514.1994.12026378
  13. Matheny PB. Improving phylogenetic inference of mushrooms with RPB1 and RPB2 nucleotide sequences (Inocybe; Agaricales). Mol Phylogenet Evol. 2005;35(1):1-20. https://doi.org/10.1016/j.ympev.2004.11.014
  14. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33(7):1870-1874. https://doi.org/10.1093/molbev/msw054
  15. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. 2013;30(4):772-780. https://doi.org/10.1093/molbev/mst010
  16. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics. 2014;30(9):1312-1313. https://doi.org/10.1093/bioinformatics/btu033
  17. Miller MA, Pfeiffer W, Schwartz T. Creating the CIPRES science gateway for inference of large phylogenetic trees. 2010 gateway computing environments workshop (GCE). New Orleans (LA): Institute of Electrical and Electronics Engineers; 2010; p. 1-8.
  18. Rambaut A. FigTree-version 1.4. 3, a graphical viewer of phylogenetic trees. Computer program distributed by the author. 2017. Available from: http://tree.bio.ed.ac.uk/software/figtree
  19. Munsell. Munsell soilcolor charts with genuine Munsell color chips. Grand Rapids (MI): Munsell Color; 2009.
  20. Wu F, Chen JJ, Ji XH, et al. Phylogeny and diversity of the morphologically similar polypore genera Rigidoporus, Physisporinus, Oxyporus, and Leucophellinus. Mycologia. 2017;109(5):749-765.
  21. Zha LS, WEN TC, Huang SK, et al. Taxonomy and biology of Cordyceps qingchengensis sp. nov. and its allies. Phytotaxa. 2019;416(1):14-24-14-24. https://doi.org/10.11646/phytotaxa.416.1.2
  22. Life Co. Catalogue of Life. 2021. Available from: https://www.catalogueoflife.org/
  23. Sung GH, Hywel-Jones NL, Sung JM, et al. Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Stud Mycol. 2007;57:5-59. https://doi.org/10.3114/sim.2007.57.01
  24. Vega FE, Goettel MS, Blackwell M, et al. Fungal entomopathogens: new insights on their ecology. Fungal Ecol. 2009;2(4):149-159. https://doi.org/10.1016/j.funeco.2009.05.001
  25. Larsen MJ, Gilbertson R. Dendrocorticium and Dentocorticium, gen. nov. (Aphyllophorales, Corticiaceae) as segregates from Laeticorticium. Nord J Bot. 1974;21:223-226.
  26. Nakasone KK, Hibbett DS, Goranova G. Neocampanella, a new corticioid fungal genus, and a note on Dendrothele bispora. Botany. 2009;87(9):875-882. https://doi.org/10.1139/B09-046
  27. Hjortstam K. A check-list to genera and species of corticioid fungi (Hymenomycetes). Windahlia. 1987;17:55-85.
  28. Miettinen O, Spirin V, Vlas ak J, et al. Polypores and genus concepts in Phanerochaetaceae (Polyporales, Basidiomycota). MycoKeys. 2016;17:1-46. https://doi.org/10.3897/mycokeys.17.10153
  29. Nakasone KK. Leptocorticium (Corticiaceae s.l., Basidiomycota): new species and combinations. Mycol Prog. 2005;4(3):251-256. https://doi.org/10.1007/s11557-006-0128-2
  30. Liu SL, Nakasone KK, Wu SH, et al. Taxonomy and phylogeny of Lopharia s.s., Dendrodontia, Dentocorticium and Fuscocerrena (Basidiomycota, Polyporales). MycoKeys. 2018;32:25-48. https://doi.org/10.3897/mycokeys.32.23641
  31. Parmasto E. Hymenochaetoid fungi (Basidiomycota) of North america. Mycotaxon. 2001;79:107-176.
  32. Dai YC. Hymenochaetaceae (Basidiomycota) in China. Fungal Divers. 2010;45(1):131-343. https://doi.org/10.1007/s13225-010-0066-9
  33. He SH, Dai YC. Taxonomy and phylogeny of Hymenochaete and allied genera of Hymenochaetaceae (Basidiomycota) in China. Fungal Divers. 2012;56(1):77-93. https://doi.org/10.1007/s13225-012-0174-9
  34. Larsson KH, Parmasto E, Fischer M, et al. Hymenochaetales: a molecular phylogeny for the hymenochaetoid clade. Mycologia. 2006;98(6): 926-936. https://doi.org/10.1080/15572536.2006.11832622
  35. Gilbertson RL, Ryvarden L. North American poly-pores vol. 2. Megasporoporia-Wrightoporia. Oslo, Norway: Fungiflora; 1987. p. 437-885.
  36. Dai YC. Changbai wood-rotting fungi 9. Three new species and other species in Rigidoporus, Skeletocutis and Wolfiporia (Basidiomycota, Aphyllophorales). Annales botanici fennici. New York (NY): JSTOR; 1998. p. 143-154.
  37. Ryvarden L, Melo I. Poroid fungi of Europe. Oslo, Norway: Fungiflora; 2014.
  38. Wagner T, Fischer M. Proceedings towards a natural classification of the worldwide taxa Phellinus s.l. and Inonotus s.l., and phylogenetic relationships of allied genera. Mycologia. 2002;94(6):998-1016. https://doi.org/10.1080/15572536.2003.11833156
  39. Miettinen O, Larsson E, Sjokvist E, et al. Comprehensive taxon sampling reveals unaccounted diversity and morphological plasticity in a group of dimitic polypores (Polyporales, Basidiomycota). Cladistics. 2012;28(3):251-270. https://doi.org/10.1111/j.1096-0031.2011.00380.x
  40. Chen JJ, Dai YC. Two new species of Physisporinus (Polyporales, Basidiomycota) from Yunnan, southwest China. Mycol Progress. 2021;20(1):1-10. https://doi.org/10.1007/s11557-020-01647-8
  41. Dai YC. Polypore diversity in China with an annotated checklist of Chinese polypores. Mycoscience. 2012;53(1):49-80. https://doi.org/10.1007/s10267-011-0134-3
  42. Li GJ, Zhao RL, Zhang CL, et al. A preliminary DNA barcode selection for the genus Russula (Russulales, Basidiomycota). Mycology. 2019;10(2):61-74. https://doi.org/10.1080/21501203.2018.1500400
  43. Adamcik S, Looney B, Cabon M, et al. The quest for a globally comprehensible Russula language. Fungal Divers. 2019;99(1):369-449. https://doi.org/10.1007/s13225-019-00437-2
  44. Park MS, Lee H, Oh SY, et al. Species delimitation of three species within the Russula subgenus Compacta in Korea: R. eccentrica, R. nigricans, and R. subnigricans. J Microbiol. 2014;52(8):631-638. https://doi.org/10.1007/s12275-014-4168-z
  45. Shimono Y, Kato M, Takamatsu S. Molecular phylogeny of Russulaceae (Basidiomycetes; Russulales) inferred from the nucleotide sequences of nuclear large subunit rDNA. Mycoscience. 2004;45(5):303-316. https://doi.org/10.1007/S10267-004-0189-5
  46. Das K, Atri N, Buyck B. Three new species of Russula (Russulales) from India. Mycosphere. 2013;4(4):707-717. https://doi.org/10.5943/mycosphere/4/4/7
  47. Li GJ, Li SM, Buyck B, et al. Three new Russula species in sect. Ingratae (Russulales, Basidiomycota) from Southern China. MycoKeys. 2021;84:103-139. https://doi.org/10.3897/mycokeys.84.68750
  48. Miller SL, Buyck B. Molecular phylogeny of the genus Russula in Europe with a comparison of modern infrageneric classifications. Mycol Res. 2002;106(3):259-276. https://doi.org/10.1017/S0953756202005610
  49. Bazzicalupo AL, Buyck B, Saar I, et al. Troubles with mycorrhizal mushroom identification where morphological differentiation lags behind barcode sequence divergence. Taxon. 2017;66(4):791-810. https://doi.org/10.12705/664.1
  50. Han JG, Choi YJ, Pfister DH, et al. Scutellinia jejuensis (Pezizales), a new species from Korea. Mycotaxon. 2010;112(1):47-53. https://doi.org/10.5248/112.47
  51. Schumacher T. The genus Scutellinia pyronemataceae. Opera Bot. 1990;101:1-107.
  52. Meihua L, Hongwei P. Scutellinia sinensis, a new spherical-spored species of Scutellinia. Acta Mycol Sin. 1996;15:98-100.
  53. Matocec N. The genus Scutellinia pezizales in Croatia III. A new species-Scutellinia tuberculata. Mycotax. 2000;76:481-488.
  54. Yao Y, Spooner B. New combinationa in Melastiza and Scutellinia (Pezizales). Mycotaxon. 1995;53:467-477.
  55. Samson RA. Paecilomyces and some allied Hyphomycetes. Stud Mycol. 1974;6:1-119.
  56. He SH, Li HJ. Hymenochaete (hymenochaetales, basidiomycota) in China 7. H. cana sp. nov. and H. denticulata new to China. Chiang Mai J Sci. 2014;41:781-788.
  57. Nunez M, Ryvarden L. New and interesting poly-pores from Japan. Fungal Divers. 1999;3:107-121.
  58. Cui BK, Dai YC. Oxyporus piceicola sp. nov. with a key to species of the genus in China. Mycotaxon. 2009;109(1):307-313. https://doi.org/10.5248/109.307
  59. Donk MA. Revisie van de nederlandse Heterobasidiomycetae en Homobasidiomycetae-Aphyllophoraceae. Meddelingen van der Nederlandse Mycologische Vereen. 1931;18-20:67-200
  60. Cui BK, Huang MY, Dai YC. A new species of Oxyporus (Basidiomycota, Aphyllophorales) from northwest China. Mycotaxon. 2006;96:207-210.
  61. Qu elet L. Quelques especes critiques ou nouvelles de la flore mycologique de France. Compte Rendu de l'Association Franc,aise pour l'Avancement des Sciences. 1895;24:616-622.
  62. Choi YJ, Shin HD, Han JG, et al. Scutellinia (Pezizales) in Korea, with a new species and eight new records. Nova Hedwigia. 2013;97(3-4):457-476. https://doi.org/10.1127/0029-5035/2013/0120