Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Morphological and Molecular Classifications of Genus Pholis

  • Lee, Sung-Hoon (College of Fisheries & Ocean Sciences, Chonnam National University) ;
  • Jang, Yo-Soon (Marine Living Resources Research Division, KORDI) ;
  • Baik, Chung-Boo (Marine Living Resources Research Division, KORDI) ;
  • Han, Kyeong-Ho (College of Fisheries & Ocean Sciences, Chonnam National University) ;
  • Myung, Jung-Goo (Marine Living Resources Research Division, KORDI) ;
  • Lee, Jin-Hee (College of Fisheries & Ocean Sciences, Chonnam National University) ;
  • Choi, Sang-Duk (College of Fisheries & Ocean Sciences, Chonnam National University) ;
  • Kim, Seon-Jae (College of Fisheries & Ocean Sciences, Chonnam National University) ;
  • Kim, Jong-Oh (College of Fisheries & Ocean Sciences, Chonnam National University) ;
  • Hwang, Jae-Ho (Eco Marine Bio Center, Chonnam National University)
  • Published : 2009.12.31
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Abstract

Morphological and molecular classifications were attempted in an effort to establish species-specific classifications of three species of the genus Pholis in Korea; these species were subjected to morphological and molecular methodologies using body measurements, RFLP, RAPD, and phylogenetic trees using the nucleotide sequences of mitochondrial 16S and 12S ribosomal DNAs, cytochrome c oxidase I, and cytochrome b. The data demonstrated that the three species of genus Pholis are distinct from each other, both morphologically and genetically.

Keywords

References

  1. Brown WM (1980) Polymorphism in mitochondrial DNA of human as reversed by restriction endonuclease analysis, Proc Natl Acad Sci USA 77: 3605-3609 https://doi.org/10.1073/pnas.77.6.3605
  2. Brown WM, George JG, and Wilson AC (1979) Rapid evolution of animal mitochondrial DNA. Proc Natl Acad Sci USA 76: 967-1971 https://doi.org/10.1073/pnas.76.2.967
  3. Brown WM, George M, and Wilson AC (1982) Mitochondrial DNA sequences of primates: tempo and mode of evolution. J Mol Evol 18: 225-239 https://doi.org/10.1007/BF01734101
  4. Carm RL and Wilson AC (1983) Length mutation in human mitochondrial DNA. Genetics 104: 699-711
  5. Felsenstein J (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783-791 https://doi.org/10.2307/2408678
  6. Hur SB, Kim DY, and Yoo JM (1984) Larval stock of Enedrias in the Yellow Sea. Bull Natl Fish Univ Pusan 24: 69-79
  7. Hur SB and Yoo JM (1983) Notes on external morphology of Enedrias nebulosa and E. fangi in Korean waters. Bull Korean Fish Soc 16: 97-102
  8. Huh SH and Kwak SN (1997) Feeding habit of Pholis nebulosa. Korean J Ichthyol 9: 22-29
  9. Kang YJ, Kim YH, and Kim WT. 1996. Age, growth and spawning of Enedrias nebulosa. J Korean Fish Soc 29: 191-196
  10. Kim KH 1995. The analysis of mtDNA using restriction endonucleases in Korean toad. Bufo bufo. Master Thesis, Inha Univ., pp. 1-25
  11. Kim IS, Choi Y, Lee CL, Lee YJ, Kim BJ, and Kim JH (2005) Illustrated Book of Korean Fishes. Kyo-Hak Publishing Co., Seoul, pp 615
  12. Kim JM, Kim DY, Yoo JM, and Huh HT (1985) Food of the larval gunnel, Enedrias fangi. Bull Korean Fish Soc 18: 484-490
  13. Lee GO, Kim SJ, and Oh MY (1999) PCR-RFLP of the cytochrome b genes in the Cheju native horse. Korean J Genetics 21: 29-34
  14. Miya M, Takeshima H, Endo H, Ishiguro NB, Inoue Jo, Mukai T, Satoh TP, Yamaguchi M, Kawaguchi A, Mabuchi K, Shirai SM, and Nishida M (2003) Major patterns of higher teleostean phylogenies: a new perspective based on 100 complete mitochondrial DNA sequences. Mol Phylogenet Evol 26: 121-138 https://doi.org/10.1016/S1055-7903(02)00332-9
  15. Palma RE and Spotorno AE (1999) Molecular systematics of marsupials based on the rRNA 12S mitochondrial gene: the phylogeny of didelphimorphia and of the living fossil microbiotheriid Dromiciops gliroides Thomas. Mol Phylogenet Evol 13: 525-535 https://doi.org/10.1006/mpev.1999.0678
  16. Saitou N and Nei M (1987) The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425
  17. Tamura K, Dudley J, Nei M, and Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24:1596-1599 https://doi.org/10.1093/molbev/msm092
  18. Upholt WB and Dawid IB (1977) Estimation of DNA sequence divergence from comparison of restriction endonucleases digests. Nucleic Acids Res 4: 1257-1265 https://doi.org/10.1093/nar/4.5.1257
  19. Zhu D, Jamieson BGM, Hugall A, and Moritz C (1994) Sequence evolution and phylogenetic signal in control region and cytochrome b sequences of rainbow fishes (Nelano taeniidae). Mol Biol evol 11: 672-683