Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
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The complete chloroplast genome of Zoysia macrostachya (Poaceae): Insights into intraspecific variations and species delimitation of the Zoysia species

  • OH, Sung-Dug (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • LEE, Seong-Kon (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • YUN, Doh-Won (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • SUN, Hyeon-Jin (Subtropical Horticulture Research Institute, Jeju National University) ;
  • KANG, Hong-Gyu (Subtropical Horticulture Research Institute, Jeju National University) ;
  • LEE, Hyo-Yeon (Subtropical Horticulture Research Institute, Jeju National University) ;
  • XI, Hong (InfoBoss Inc. and InfoBoss Research Center) ;
  • PARK, Jongsun (InfoBoss Inc. and InfoBoss Research Center) ;
  • LEE, Bumkyu (Department of Environmental Science & Biotechnology, Medicical Science, Jeonju University)
  • Received : 2021.08.05
  • Accepted : 2021.09.23
  • Published : 2021.09.30
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Abstract

The complete chloroplast genome of Zoysia macrostachya Franch. & Sav. isolated in Korea is 135,902 bp long (GC ratio is 38.4%) and has four subregions; 81,546 bp of large single-copy (36.3%) and 12,586 bp of small single-copy (32.7%) regions are separated by 20,885 bp of inverted repeat (44.1%) regions, including 130 genes (83 protein-coding genes, eight rRNAs, and 39 tRNAs). Thirty-nine single nucleotide polymorphisms and 11 insertions and deletion (INDEL) regions were identified from two Z. macrostachya chloroplast genomes, the smallest among other Zoysia species. Phylogenetic trees show that two Z. macrostachya chloroplast genomes are clustered into a single clade. However, we found some incongruency with regard to the phylogenetic position of the Z. macrostachya clade. Our chloroplast genome provides insights into intraspecific variations and species delimitation issues pertaining to the Zoysia species.

Keywords

Acknowledgement

This work was carried out with the support of "Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ014235)" and "Next-Generation BioGreen 21 Program (Project No. PJ 01368504)" Rural Development Administration, Republic of Korea.

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