• Title/Summary/Keyword: phase II system

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Enhanced Partitioning of Proteins in Metal-Affinity Aqueous Two-Phase Systems (금속 친화성 액 이상분계 시스템에서 단백질의 분배 향상)

  • Chung, Bong-Hyun;Park, Young-Hoon
    • Microbiology and Biotechnology Letters
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    • v.21 no.4
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    • pp.336-341
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    • 1993
  • In metal-affinify aqueous two-phase systems, protein partitioning is affected by a variety of parameters such as pH, the number of surface-accessible histidines, and the amount and partition coefficient of metallated polythylene glyco(PEG) ligand. To enhance partitioning of proteins with surface-accessible histidines, we have synthesized and used a (Cu(II)-ininodiacetic acid)$_2$-PEG20,000($Cu(II)_2IDA_2$-PEG20,000) as well as Cu(II)IDA-PEG5,000 as an affinity ligand. The partition coefficient of $Cu(II)_2-IDA_2$-PEG20,000 in a PEG5,000/dextran two-phase system was 30.1, which corresponded to a 3.8-fold increase over that of Cu(II)IDA-PEG5,000. The partitioning experiments were performed on four proteins, horse cytochrome c, S. cerevisiae cytochrome c, horse myoglobin, and sheep myoglobin. Partitioning of proteins which convey surface-accessible histidines was enhanced dramatically by the addition of $Cu(II)_2IDA_2$-PEG20,000 ligand. These results demonstrate that enhanced partitioning of metal-binding proteins in an aqueous two -phase system can by achieved by using an appropriate metallated PEG ligand.

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A Computer Program for System Reliability Prediction (시스템의 신뢰성(信賴性) 예측(豫測)을 위한 컴퓨터 프로그램)

  • Kim, Yeong-Hwi;Choe, Mun-Gi
    • Journal of Korean Institute of Industrial Engineers
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    • v.1 no.2
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    • pp.51-56
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    • 1975
  • A computer program for computing complex system reliability is described. The program is composed of three phases : Phase I program reduces all series, parallel and series-parallel components and subsequently obtains an irreducible non-series-parallel system. Phase II program enumerates all the possible paths from the source to the sink of the graph. Phase III program then computes system reliability based on the information obtained by the Phase II program. The program is based on a modified version of the algorithm published in [6]. An example of the use of the computer program is given.

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Development of Working Tractor with Four-Type Wheel Steering System II(Development of Four-Type Wheel Steering System) (4방식 조향장치를 적용한 관리 작업차 개발 II(4방식 조향장치 개발))

  • Cho Hyun-Deog
    • Transactions of the Korean Society of Machine Tool Engineers
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    • v.14 no.3
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    • pp.81-86
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    • 2005
  • The agricultural working tractor of this study is equipped with 4 wheel driving system developed in study 1 and 4-type wheel steering system. The wheel steering system has four type of steering methods that are front wheel steering, rear wheel steering, 4 wheel steering with opposite phase, and 4 wheel steering with corresponding phase. This study introduces the hydraulic circuit of the 4-type wheel steering system and the construction of working tractor. Judging from the field test results of the developed working tractor, it is apparent that 4-type wheel steering system has many advantages when driving in a narrow corral.

Changes of Surface Electromyography on Frontalis Muscle during Electro-Acupuncture Stimulation of Abdomen or Legs - A Pilot Study for Clinical Approach to Autonomic Nervous System Changes - (복부 및 사지 부위의 전기침 자극이 전두근에서 측정한 SEMG 변화에 미치는 영향 - 자율신경계의 변화에 대한 임상적 접근을 위한 사전 연구 -)

  • Je, Jun-Tae;Choi, San-Ho;Shin, Sun-Ho;Lim, Jin-Young;Lee, Sang-Kwan
    • The Journal of Internal Korean Medicine
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    • v.33 no.1
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    • pp.14-25
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    • 2012
  • Objectives : To observe physiological changes during clinical acupuncture treatment. Methods : We recruited 40 healthy volunteers who had experienced an acupuncture treatment at least once within the past three years. The experimental group was divided into four groups according to the needling site and frequency of electrical stimulation. Sites consisted of abdomen and legs. Frequencies consisted of 100 Hz and 2 Hz. The procedures of experimental treatment consisted of seven phases, Resting I phase (Resting I), Needle insertion phase (Insertion), Maintenance of needle insertion I phase (Maintain I), Electrical stimulation phase (ES), Maintenance of needle insertion II phase (Maintain II), Needle removal phase (Removal) and Resting II phase (Resting II). We measured the surface electromygraphy (SEMG) through an electrode on the frontalis muscle during all phases consecutively. Results : When SEMGs of all seven phases were analyzed, they significantly increased or decreased according to phases. SEMGs of Insertion, Maintain I, ES and Maintain II phase significantly increased more than RestingI in abdomen and legs groups. SEMGs of the abdomen group were measured as being $4.78{\pm}0.74{\mu}V$ on Resting I, $16.48{\pm}3.97{\mu}V$ on Insertion, $46.31{\pm}10.56{\mu}V$ on Maintain I, $45.88{\pm}9.72{\mu}V$ on ES, $45.56{\pm}9.69{\mu}V$ on Maintain II, $18.76{\pm}3.05{\mu}V$ on Removal, and $3.75{\pm}0.65{\mu}V$ on Resting II. SEMGs of the legs group were measured as being $3.34{\pm}0.35{\mu}V$ on Resting I, $12.11{\pm}1.76{\mu}V$ on Insertion, $36.74{\pm}6.99{\mu}V$ on Maintain I, $33.57{\pm}6.30{\mu}V$ on ES, $32.66{\pm}6.03{\mu}V$ on Maintain II, $14.08{\pm}2.15{\mu}V$ on Removal, and $2.88{\pm}0.32{\mu}V$ on Resting II. Conclusions : SEMG changed differently according to processes of acupuncture. Electrical stimulation showed different change of SEMG. Thus, acupuncture treatment may change the status of the autonomic nervous system.

Separation of Functionalized Heterocyclic Compounds by High Performance Liquid Chromatography (II) (고성능 액체 크로마토그래피에 의한 기능성 헤테로 고리 화합물의 분리(II))

  • Cho, Yun Jin;Lee, Young Cheol;Lee, Kwang-PiII;Park, Keung-Shik
    • Analytical Science and Technology
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    • v.11 no.4
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    • pp.292-296
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    • 1998
  • Normal phase or reversed phase liquid chromatographic separation of isoquinoline of heterocyclic compounds and structural isomers of external substituents, $COOCH_3$, CN and $CH_3$ has been carried out by using several different columns and various mobile phases. From this results, the order of elution of heterocyclic compounds appears to depend on the solvent effect with kinds of mobile phases. Retention mechanism of normal phase system for 2-methylindoline, 2-methylindole, benzoxazole and benzothiazole was also studied depending on adsorption strength between solute and stationary phase of column. However, retention factors of reversed phase system were found on hydrophobic interaction with solvophobic effect.

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Study of Phase Transition of Copper(II)-phthalocyanine using a Near Field Scanning Microwave Microscope (근접장 마이크로파 현미경을 이용한 Copper(II)-phthalocyanine의 Phase Transition 연구)

  • Park, Mie-Hwa;Yoo, Hyun-Jun;Yun, Soon-Il;Lim, Eun-Ju;Lee, Kie-Jin;Cha, Deok-Joon;Lee, Young-San
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.17 no.6
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    • pp.641-646
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    • 2004
  • We report the changes of the microwave reflection coefficients S$_{11}$ of copper(II)-phthalocyanine (CuPc) thin films by using a near-field microwave microscope(NSMM) in order to understand the phase transition of CuPc. For a NSMM system, a high-quality microstrip resonator coupled with a dielectric resonator was used. CuPc thin films were prepared on the pre-heated glass substrates using a thermal evaporation method. The reflection coefficients S$_{11}$ of CuPc thin films were changed by the dependence on the substrate pre-heating temperatures. By comparing reflection coefficient S$_{11}$ and crystal structures, we found the phase transition of CuPc thin films from $\alpha$-phase to $\beta$-phase at the substrate heating temperature 200 $^{\circ}C$./TEX>.

Selection of Voltage Vectors in Three-Level Five-Phase Direct Torque Control for Performance Improvement

  • Tatte, Yogesh N.;Aware, Mohan V.
    • Journal of Power Electronics
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    • v.16 no.6
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    • pp.2162-2172
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    • 2016
  • This paper presents a Direct Torque Control (DTC) strategy for the five-phase induction motor driven by a three-level five-phase inverter in order to improve the performance of the five-phase induction motor. In the proposed DTC technique, only 22 voltage vectors out of 243 available voltage vectors in a three-level five-phase inverter are selected and are divided in 10 sectors each with a width of $36^{\circ}$. The four different DTC combinations (DTC-I, II, III and IV) for a three-level five-phase induction motor drive are investigated for improving the performance of five-phase induction motor. All four of the DTC strategies utilize a combination of the same large and zero voltage vectors, but with different medium voltage vectors. Out of these four techniques, DTC-II gives the best performance when compared to the others. This DTC-II technique is analyzed in detail for improvements in the performance of five-phase induction motor in terms of torque ripple, x-y stator flux and Total Harmonics Distortion (THD) of the stator phase current when compared to its two-level counterparts. To verify the effectiveness of the proposed three-level five-phase DTC control strategy, a DSP based experimental system is build. Simulation and experimental results are provided in order to validate the proposed DTC technique.

Simplification of ASM No. 1 Using Aerobic-Anoxic SBR (호기-무산소 SBR 반응조를 이용한 ASM No. 1 모델의 간략화)

  • Kim, Shin Geol;Choi, In Su;Koo, Ja Yong
    • Journal of Korean Society of Water and Wastewater
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    • v.21 no.4
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    • pp.409-420
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    • 2007
  • ASM No. 1 is a very useful model to analyze wastewater treatment system removing organic carbon and nitrogen material. But it isn't adequate to control the wastewater treatment system with real time since it has many material divisions and parameters. So, the purpose of this study is the simplification of ASM No. 1 to control the wastewater treatment system. ASM No. 1 was changed with the model which has 3 material divisions(COD, $NH_4{^+}$, $NO_3{^-}$) and two phases(Aerobic and Anoxic condition). SBR was running with two phases(Phase I and II). Phase II running 20 minutes with aerobic time was used for deciding model parameters and Phase I running 12 minutes with aerobic time was used for proving the simplified model. The simplified model was compared with ASM No. 1 using data of Phase I and II. As a result of model comparison, the simplified model has enough ability to express the variation of $NH_4{^+}$ compound.

KTX-II development using systems engineering (신규고속철도차량(KTX-II)의 시스템엔지니어링 적용)

  • Cha, Jae-Hwan;Chung, In-Soo;Jo, Kwang-Woo
    • Journal of the Korean Society of Systems Engineering
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    • v.4 no.1
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    • pp.31-34
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    • 2008
  • As a supply contract for 100 new high-speed rolling stocks(KTX-II) was made 8th June 2006, System Engineering process has been applied to introduce the high-speed rolling stocks successfully and to manage the total life cycle systematically from Pre-concept Phase, Contract Phase, Preliminary and Critical Design Review, Production, Test and Verification, Acceptance and Operation to assurance for a flaw.

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A Study on the PR shaped SQAM error rate with carrier phase error (PR Shape된 SQAM의 오율에 반송파위상오차가 미치는 영향에 대한 연구)

  • 박용우;이형재
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.9 no.1
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    • pp.11-17
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    • 1984
  • A study is presented showing the effect of carrier phase error on the error-rate of a (Class I, II, IV)PR shaped SQAM digital communication system. A simple upper bound on the probabilty of error as a function of phase error is derived and compared to one another. The results show that the three system can be used appropriately if carrier phase error is less than 3$^{\circ}$. However, if phase error is larger than 3, the PR class I is the best choice.

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