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Effect of insect-resistant genetically engineered (Bt-T) rice and conventional cultivars on the brown planthopper (Nilaparvata lugens Stål)

  • Sung-Dug, Oh;Eun Ji, Bae;Kijong, Lee;Soo-Yun, Park;Myung-Ho, Lim;Doh-Won, Yun;Seong-Kon, Lee;Gang-Seob, Lee;Soon Ki, Park;Jae Kwang, Kim;Sang Jae, Suh
    • Korean Journal of Agricultural Science
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    • v.49 no.3
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    • pp.511-520
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    • 2022
  • Insect-resistant transgenic rice (Bt-T) expresses a toxic protein (mcry1Ac1) derived from the soil bacterium Bacillus thuringiensis found in the rice cultivar Dongjin with an insecticidal property against rice leaf roller (Cnaphalocrocis medinalis). In this study, to investigate the impact of Bt-T on non-target organisms, the feed and oviposition preferences and biological parameters of brown planthopper (Nilaparvata lugens Stål) were comparatively analyzed in four rice cultivars: Dongjin (parent variety), Ilmi (reference cultivar), Chinnong (brown planthopper resistant cultivar) and Bt-T. In the Bt-T and Dongjin cultivars, the feed preferences were 32.4 ± 8.3 and 34.1 ± 6.8%, and the oviposition preferences were 32.5 ± 5.1 and 30.0 ± 5.3% respectively, and there was no statistical significance between these rices. Additionally, in the Bt-T and Dongjin cultivars, the total lifespans from egg to adult were 39.5 ± 6.9 and 40.0 ± 5.8 days, and the weights of adult females were 1.78 ± 0.14 and 1.72 ± 0.16 mg, respectively. Therefore, there was no statistical difference in the biological parameters between these two varieties. Overall, the results indicate that the insect-resistant transgenic rice (Bt-T) did not negatively affect the reproduction and life cycle of brown planthopper, a non-target organism.

The Characteristics of Figures Who has the Imaginary Companion in the Picture Book (그림책에서 상상친구를 가진 인물의 특성)

  • Park, Hyun-Kyung;Bong, Jin-Young
    • The Journal of the Korea Contents Association
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    • v.22 no.1
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    • pp.337-347
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    • 2022
  • This research aims to analyze the gender, sibling, and characteristics of figures who created the imaginary friends among 30 picture books published in Korea. At first, as a result of the research, a total of 35 figures of the 30 books had imaginary friends, of which 24 figures were women and 11 were men, with women more than twice as many as men. Secondly, in many cases, the only child in picture books had the imaginary companion, but in case of the figures who have brothers or sisters, The imaginary companion visited them more frequently to different gender rather than to same gender. Looking at the frequency of appearance of imaginary companion, the eldest, younger brother, and no appearance appeared in order. Third, as a result of examining the personality of figures who created the imaginary companion, their extroversion or introversion has no meaningful correlation each other. These results are meaningful as reference data for understanding children with imaginary friends in reality as picture books reflect the reality of children with imaginary friends and show various situations of infant with imaginary friends in a balanced manner.

Comparison and analysis of spatial information measurement values of specialized software in drone triangulation (드론 삼각측량에서 전문 소프트웨어의 공간정보 정확도 비교 분석)

  • Park, Dong Joo;Choi, Yeonsung
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.15 no.4
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    • pp.249-256
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    • 2022
  • In the case of Drone Photogrammetry, the "pixel to point tool" module of Metashape, Pix4D Mapper, ContextCapture, and Global MapperGIS, which is a simple software, are widely used. Each SW has its own logic for the analysis of aerial triangulation, but from the user's point of view, it is necessary to select a SW by comparative analysis of the coordinate values of geospatial information for the result. Taking aerial photos for drone photogrammetry, surveying GCP reference points through VRS-GPS Survey, processing the acquired basic data using each SW to construct ortho image and DSM, and GCPSurvey performance and acquisition from each SW The coordinates (X,Y) of the center point of the GCP target on the Ortho-Image and the height value (EL) of the GCP point by DSM were compared. According to the "Public Surveying Work Regulations", the results of each SW are all within the margin of error. It turned out that there is no problem with the regulations no matter which SW is included within the scope.

Monitoring changes in the genetic structure of Brown Tsaiya duck selected for feeding efficiency by microsatellite markers

  • Yi-Ying Chang;Hsiu-Chou Liu;Chih-Feng Chen
    • Animal Bioscience
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    • v.36 no.3
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    • pp.417-428
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    • 2023
  • Objective: Few studies have genetically monitored chickens over time, and no research has been conducted on ducks. To ensure the sustainable management of key duck breeds, we used microsatellite markers to monitor Brown Tsaiya ducks over time genetically. Methods: The second, fourth, sixth to eighth generations of the Brown Tsaiya duck selected for feeding efficiency and control lines were included in this study to investigate the genetic variations, effective population size, population structure and the differentiation between populations over time with 11 microsatellite markers derived from Brown Tsaiya duck. Results: The results showed there were a slight decrease in the genetic variations and an increase in within-population inbreeding coefficient (FIS) in both lines, but no consistent increase in FIS was observed in each line. The effective population size in the second and eighth generations was 27.2 for the selected line and 23.9 for the control line. The change in allele richness showed a downward trend over time, and the selected line was slightly lower than the control line in each generation. The number of private alleles (Np) in the selected line were higher than in the control line. Moderate differentiation was observed between the second and eighth generations in the selected line (FST = 0.0510) and the control line (FST = 0.0606). Overall, differentiation tended to increase with each generation, but genetic variation and structure did not change considerably after six generations in the two lines. Conclusion: This study provides a reference for poultry conservation and helps to implement cross-generation genetic monitoring and breeding plans in other duck breeds or lines to promote sustainable management.

Comparative Evaluation of Culture Media for Quantification of Lactic Acid Bacteria in Various Dairy Products

  • Eiseul Kim;Shin-Young Lee;Yoon-Soo Gwak;Hyun-Jae Kim;Ik-Seon Kim;Hyo-Sun Kwak;Hae-Yeong Kim
    • Microbiology and Biotechnology Letters
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    • v.51 no.1
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    • pp.10-17
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    • 2023
  • Dairy products are extensively used as carriers of probiotic strains that have potential health benefits. Assessment of the viability of probiotic strains during manufacturing is important to ensure that products meet recommended levels. Hence, the method for accurately quantifying lactic acid bacteria (LAB) in probiotic or dairy products is required. The present study aims to examine the performance of de-Man Rogosa Sharpe (MRS), plate count agar with bromocresol purple (PCA with BCP), and glucose blood liver (BL) agars recommended in the Korea Food Code guidelines for counting LAB. Analysis of the performance of culture media containing 19 lactic acid bacterial species commonly encountered in probiotic and dairy products showed no statistically significant difference between 18 reference strains and three culture media (p > 0.01). Furthermore, the suitability of three culture media was verified for the quantitative assessment of LAB in 25 probiotic and dairy products. The number of LAB in three culture media was determined to be more than 107 colony-forming unit (CFU)/ml for fermented milk products and 108 CFU/ml for condensed fermented milk and probiotic products, indicating that they all satisfied the Korea Food Code guidelines. Moreover, there was no statistically significant difference in the amount of LAB counted in all three culture media, suggesting that they can be used to isolate or enumerate LAB in commercial products. Finally, three culture media will be useful for isolating and enumerating LAB from fermented foods as well as gut microflora.

Automated radiochemical synthesis of [18F]FET on TRACERlab FX2N module and its quality control

  • Dong Hyun Kim;Eun-bi Shin;Iljung Lee;Heejung Kim;Kyo Chul Lee;Kyeong Min Kim;Joo Hyun Kang;Sang Moo Lim
    • Journal of Radiopharmaceuticals and Molecular Probes
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    • v.7 no.2
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    • pp.147-152
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    • 2021
  • C-11 Radiolabeled amino acid-based radiopharmaceuticals such as [11C]MET for brain tumor PET imaging have limitations due to their short half-life (20 min). F-18 radiolabeled amino acid derivatives have been developed to overcome for the short half-life, one of which is [18F]FET. Brain tumor imaging using [18F]FET showed high uptake in tumor region and no non-specific uptake in inflammatory tissue, which was useful in discriminating the difference between inflammation and tumor especially. In this study, [18F]FET was synthesized using an automatic synthesis module and quality tests were carried out including enantiomeric purity analysis with reference compounds. Radiochemical yield was 50.3 ± 4.9% (n=7, decay-corrected) with molar activity of 76 ± 17 GBq/mmol. The radiochemical purity of >99%. Enantiomeric purity of [18F]FET using chiral HPLC analysis showed >99%, which was confirmed by co-injection with the L-FET and D-FET authentic standards. [18F]FET was prepared with high radiochemical yield and molar activity including no racemate mixture.

Can ultra-low-dose computed tomography reliably diagnose and classify maxillofacial fractures in the clinical routine?

  • Gerlig Widmann;Marcel Dangl;Elisa Lutz;Bernhard Fleckenstein;Vincent Offermanns;Eva-Maria Gassner;Wolfgang Puelacher;Lukas Salbrechter
    • Imaging Science in Dentistry
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    • v.53 no.1
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    • pp.69-75
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    • 2023
  • Purpose: Maxillofacial trauma predominantly affects young adults between 20 and 40 years of age. Although radioprotection is a legal requirement, the significant potential of dose reduction in computed tomography (CT) is still underused in the clinical routine. The objective of this study was to evaluate whether maxillofacial fractures can be reliably detected and classified using ultra-low-dose CT. Materials and Methods: CT images of 123 clinical cases with maxillofacial fractures were classified by two readers using the AOCOIAC software and compared with the corresponding results from post-treatment images. In group 1, consisting of 97 patients with isolated facial trauma, pre-treatment CT images at different dose levels (volumetric computed tomography dose index: ultra-low dose, 2.6 mGy; low dose, <10 mGy; and regular dose, <20 mGy) were compared with post-treatment cone-beam computed tomography (CBCT). In group 2, consisting of 31 patients with complex midface fractures, pre-treatment shock room CT images were compared with post-treatment CT at different dose levels or CBCT. All images were presented in random order and classified by 2 readers blinded to the clinical results. All cases with an unequal classification were re-evaluated. Results: In both groups, ultra-low-dose CT had no clinically relevant effect on fracture classification. Fourteen cases in group 2 showed minor differences in the classification code, which were no longer obvious after comparing the images directly to each other. Conclusion: Ultra-low-dose CT images allowed the correct diagnosis and classification of maxillofacial fractures. These results might lead to a substantial reconsideration of current reference dose levels.

Comparison of protein and amino acid content in selected cultivars of non-glutinous rice in Korea

  • So-Jung, Kim;Min Joo, Kim;Su Hyeon, Lee;Youngmin, Choi;Sung, Jeehye;Dongwon, Seo
    • Korean Journal of Agricultural Science
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    • v.48 no.4
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    • pp.1015-1026
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    • 2021
  • The objective of this study was to determine amino acid and protein contents of brown and milled non-glutinous rice of 13 cultivars in Korea. Protein contents of MRs (milled rices) were in order of Haepum (7.27%) > Hanareum No. 4 (7.14%) > Odae (6.84%). Protein contents of BRs (brown rices) were in order of Haepum (7.68%) > Odae (7.63%) > Hanareum No. 4 (7.60%). The amino acid content was the highest in Haepum (MR 5.76%, BR 6.49%), followed by Haedeul (MR 5.71%, BR 6.30%), and Odae (MR 5.63%, BR 6.29%). The essential amino acid contents of non-glutinous rices were in order of Haepum (MR 2.34%, BR 2.57%) > Haedeul (MR 2.31%, BR 2.48%) > Odae (MR 2.20%, BR 2.56%). The contents of amino acid and protein in BRs were considerably higher than those in MRs. Protein and most of amino acid contents were higher in Haepum than the other cultivars. The certificated reference material (CRM) 1849a (infant/adults nutritional formular) from National Institute of Standard and Technology (NIST) was used as the test sample to determine the precision and accuracy of the analytical method. The regression analyses revealed good correlations (correlation coefficient), greater than 0.99. The recovery values of the amino acids ranged from 93.17 to 99.59%. The limit of detection (LODs) ranged from 0.01 - 0.07 mg·100 g-1 and the limit of quantitation (LOQs) ranged from 0.03 - 0.21 mg·100 g-1 for all analytes.

A Study on the Application of Security Reinforcement Technology Reflecting Zero Trust Principles (제로 트러스트 원리를 반영한 보안 강화 요소 기술 적용 방안 연구)

  • DA-IN Lee;Hoo-Ki Lee
    • Convergence Security Journal
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    • v.22 no.3
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    • pp.3-11
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    • 2022
  • With increasingly sophisticated cyber threats, accelerating cloud adoption, and the adoption of remote and hybrid work environments due to the COVID-19 pandemic, the traditional security model, in which many businesses implicitly trust everything within their boundaries, is changing without boundaries, allowing data and users The concept of zero trust is getting more and more attention as the fact that it is not suitable for today's increasingly decentralized environment has been highlighted. Zero Trust is a cyber security model on the premise that 'no one trusts'. In principle, there is no safe area or user in the entire system, and internal users are also verified. is a way As telecommuting becomes commonplace due to the COVID-19 pandemic, and existing cyber security measures are facing limitations, Zero Trust technology is drawing more attention. Accordingly, it is expected that the Korean government will also check the status of acceptability of the domestic public and private sectors and tasks that need improvement when introducing Zero Trust with reference to the NIST standard. In this paper, the basic principles, philosophy, and considerations of Zero Trust and Zero Trust are explained, and practical basic measures to strengthen security by combining Zero Trust technology are presented.

A Case Report of UDCA and Injinho-tang Co-administration for Liver Damage Caused by Rifampin (Rifampin으로 인한 간손상에 UDCA와 인진호탕 병용투여 증례 보고)

  • Seyeon Lee;Hansung Yoon;Kibeom Ku;Mariah Kim;Irang Nam;Minhwa Kim;Changwoo Han
    • The Journal of Internal Korean Medicine
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    • v.44 no.5
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    • pp.1011-1016
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    • 2023
  • We have experienced a case in which herbal medicine was administered to treat drug-induced liver damage and would like to introduce it. A 49-year-old man exhibited a positive result in the interferon-gamma release assay. He had never suffered from tuberculosis in the past, and the route and time of infection could not be confirmed. He had no respiratory or systemic symptoms suggestive of active tuberculosis, and a chest X-ray examination showed no active lung lesions, so he was diagnosed with latent tuberculosis infection. He was confirmed to be within the normal range in the liver function test, renal function test, and complete blood cell count test, and started taking rifampin (600 mg qd). In the screening test performed on the 19th day of taking the drug, other test items were normal, but alanine aminotransferase (ALT) increased to 50 U/L (reference value: 4-40 U/L). In a test performed on the 29th day of taking the drug, ALT was clearly elevated to 102 U/L. Ursodeoxycholic acid and Injinho-tang were taken together with rifampin, and the patient's progress was observed. In a test performed 14 days later, ALT decreased to 26 U/L, within the normal range. It is presumed that Injinho-tang may have partially contributed to alleviating liver damage in this case.